- 标题
- 摘要
- 关键词
- 实验方案
- 产品
-
Could a chelant improve the effect of curcumin-mediated photodynamic antimicrobial chemotherapy against dental intact biofilms?
摘要： To our knowledge, there is still no evidence in relation to the combination of curcumin with chelants to improve the effects of antimicrobial photodynamic therapy (aPDT) on complex dental caries biofilms. Therefore, the aim of this study was to evaluate the antimicrobial effect of curcumin-ethylenediaminetetraacetic acid (EDTA)-mediated aPDT on the vitality of intact biofilms of dentin caries microcosms. Biofilms were grown on glass slabs in McBain medium plus 1% sucrose in microaerophily at 37 °C for 5 days. Then, biofilms were treated with associations of 600 μmol L?1 curcumin combined or not with 1% EDTA and 37.5 or 75 J cm?2 LED (455 nm). The vitality was determined by a confocal laser scanning microscopy (CLSM) after staining biofilms with a mixture of 2.5 g L?1 fluorescein diacetate and 0.25 g L?1 ethidium bromide. Statistical analysis was conducted by Kruskal-Wallis and post hoc Dunn’s test (P < 0.05). Three treatments were able to reduce the vitality of overall biofilms: curcumin + 75 J cm?2 LED, curcumin-EDTA + 37.5 J cm?2 LED, and curcumin-EDTA + 75 J cm?2 LED. Also, the vitality of inner layers of biofilms was significantly reduced only after the combination of aPDT with EDTA. Therefore, the association of curcumin and EDTA improved the antimicrobial effect of aPDT on dentin caries microcosms, considering the application of lower light densities and deeper layers of biofilms.
关键词： Curcumin,Biofilm,Ethylenediaminetetraacetic acid,Dental caries,Photochemotherapy
更新于2025-09-23 15:23:52
-
Could being chlorhexidine an adequate positive control to antimicrobial photodynamic therapy in in vitro studies?
摘要： Background: Chlorhexidine digluconate (CHX) is commonly applied as positive control of new antimicrobials, because it is considered the gold-standard for chemical plaque control. In this sense, the aim of this study was to compare the effect of treatments with curcumin-mediated aPDT and CHX in relation to the viability of specific microorganism groups in two distinct times (immediately and 24h later). Methods: Dentin caries microcosms were grown on bovine dentin discs (37oC, anaerobiosis) for 3 days in the Active Attachment Amsterdam Biofilm Model. The biofilms were treated with 300 μM curcumin and 75 J.cm-2 LED, or 0.06% and 0.12% CHX. Then, total microorganisms, total streptococci, mutans streptococci, and total lactobacilli counts were determined. The statistical analysis was conducted by Kruskal-Wallis and post-hoc Dunn's tests (P<0.05). Results: Curcumin-mediated aPDT (C+L+), 0.06% and 0.12% CHX reduced mutans streptococci counts (0.19, 0.10 and 0.07 log10 respectively) in the immediate analysis. After 24h, it was observed a re-growth of microorganisms treated by curcumin-mediated aPDT, whereas both CHX concentrations demonstrated a decrease of the viable microorganisms. Conclusion: This study confirmed the substantive effect of CHX and the immediate effect of aPDT. The use of a neutralizer solution was important to block the substantivity of CHX and permit its fair comparison with aPDT, allowing its use as a positive control in further studies.
关键词： Dental Caries,Oral Biofilms,Photochemotherapy,Chlorhexidine
更新于2025-09-23 15:21:01
-
Effects of 10,600?nm Carbon Dioxide Laser on Remineralizing Caries: A Literature Review
摘要： Objective: To study the effects of carbon dioxide (CO2) lasers (k = 10,600 nm) on remineralizing dental caries. Methods: This study involved performing a systematic search of English articles archived in the PubMed, Scopus, and Web of Science databases. The keywords used to identify the relevant articles were ((CO2 laser) OR (carbon dioxide laser)) AND ((dental caries) OR (tooth remineralization)). Publications before 2019 were selected. The titles and abstracts of the initially identi?ed articles were screened. Duplicate records, reviews, and irrelevant studies were removed. Full texts were retrieved for publications that studied the effects of CO2 lasers on remineralizing dental caries. Results: The search identi?ed 543 potentially relevant publications. A total of 285 duplicate records were removed. Sixteen articles were included in this review. Four studies reported that CO2 lasers inhibited bacterial growth. The growth of cariogenic bacteria, mainly Streptococcus mutans, on an irradiated tooth surface was slower compared with nonirradiated ones. Four studies investigated the reduction of the demineralization of enamel with cariogenic challenge. They found that CO2 lasers reduced the carbonate content of mineralized tissues and increased the microhardness of enamel. Nine studies used CO2 lasers associated with topical ?uorides in remineralizing dental caries. The results of the synergistic effect of laser irradiation and ?uoride application with regard to the inhibition of caries progression varied among these studies, whereas laser irradiation could enhance ?uoride uptake to demineralized mineral tissues. Conclusions: CO2 laser irradiation increased acid resistance and facilitated the ?uoride uptake of caries-like lesions. In addition, it reduced the growth of cariogenic bacteria.
关键词： carbon dioxide lasers,dental caries,?uoride,review,remineralization
更新于2025-09-19 17:13:59