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Multifaceted Protocol in Biotechnology || Characterization of Electrochemical Transducers for Biosensor Applications
摘要： Biosensors are devices that detect and report the presence or quantity of a particular analyte. Among the biosensor components, a physicochemical transducer measures physical and chemical changes from analyte-recognition interactions where products, by-products, intermediates, or physical changes are converted into a measurable signal. The character of the transducer determines the performance of a biosensor; hence the characterization of the transduction is crucial in the design of a biosensor. This chapter describes electrochemical characterization of the transducer layer of a biosensor via cyclic voltammetry.
关键词： Effective surface area,Randles-Sevcik,Electrochemical transducer,Cyclic voltammetry,Chronoamperometry,Biosensors
更新于2025-09-19 17:15:36
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A glassy carbon electrode modified with TiO2(200)-rGO hybrid nanosheets for aptamer based impedimetric determination of the prostate specific antigen
摘要： TiO2(200)-rGO hybrid nanosheets were synthesized starting from TiO2, rGO and NaOH solid powders via a scalable hydrothermal process. The weight ratio of TiO2-GO was found to be crucial on the crystal growth and biosensor properties of the final hybrid nanosheets. They were characterized by means of SEM, FESEM-EDX, XRD, XPS, Raman and FTIR spectroscopies in order to verify the formation of very thin TiO2 anatase nanosheets with an orientation of the anatase crystal structure towards the (200) plane. The free active sites of TiO2 structure and the large surface of the 2D graphene structure strongly facilitate charge transport confirmed by BET-BJH analyses. Compared to pure AuNPs, rGO and TiO2, the hybrid nanosheet modified electrode represents the most sensitive aptasensing platform for the determination of PSA. The detection was based on that the variation of electron transfer resistance (Rct) at the modified electrode surface in a solution containing 3.0 mmol L?1 [Fe(CN)6]3?/4- as a redox probe and 0.1 mol L?1 KCl as supporting electrolyte. The detection limit of the sensor is 1 pg mL?1, and the sensor can be operated up to 30 days. It was applied to the analysis of PSA levels in spiked serum samples obtained from patients with prostate cancer. Data compare well with those obtained by an immunoradiometric assay.
关键词： X-ray photoelectron spectroscopy,Effective surface area,Electrochemical impedance spectroscopy,Aptasensor,Voltammetry,Work function
更新于2025-09-04 15:30:14