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Establishment of optimal exercise therapy using near-infrared spectroscopy monitoring of tissue muscle oxygenation after therapeutic angiogenesis for patients with critical limb ischemia: A multicenter, randomized, controlled trial
摘要： Critical limb ischemia (CLI) is a potentially life-threatening condition that involves severely reduced blood flow to the peripheral arteries due to arteriosclerosis obliterans (ASO) of the limbs or a similar condition. CLI patients must undergo revascularization to avoid amputation of the lower limbs and improve their survival prognosis. However, the outcomes of conventional surgical revascularization or endovascular therapy are inadequate; therefore, establishing further effective treatment methods is an urgent task. We perform therapeutic angiogenesis using autologous bone marrow-derived mononuclear cells in clinical practice and demonstrated its safety and efficacy for CLI patients for whom conventional treatments failed or are not indicated. Exercise therapies must be devised for CLI patients who have undergone therapeutic angiogenesis to save their limbs and improve survival. Because evidence regarding the efficacy and safety of exercise therapy for CLI patients is lacking, we plan to perform a prospective trial of the efficacy and safety of optimal exercise therapy following therapeutic angiogenesis for CLI patients.
关键词： Optimal exercise therapy,Critical limb ischemia,Near-infrared spectroscopy,Arteriosclerosis obliterans,Tissue muscle oxygen saturation,Therapeutic angiogenesis
更新于2025-09-23 15:19:57
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Low-level laser therapy prevents muscle apoptosis induced by a high-intensity resistance exercise in a dose-dependent manner
摘要： Apoptosis is a highly regulated form of cell death that is characterized by definite morphological, biochemical, and molecular events. Data indicate that acute exercise affects apoptotic signals in skeletal muscle and that the apoptotic response is strongly connected to strenuous exercise. This idea is supported by clinical and experimental studies showing that apoptosis occurs after the variety of exercise types as evidenced in running, swimming, and resistance exercise (RE). Although apoptosis induced by exercise may be a normal process used to remove partially damaged cells, it is possible that excessive exercise (e.g., high effort load) causes extensive cell death. Therefore, therapeutic interventions could be tested to attenuate this process in skeletal muscle. In this regard, photobiomodulation (PBM) may be suggested as a useful tool to counteract undesirable effects of exercise. This is based, for example, on studies showing that the application of low-level laser therapy (LLLT) before RE can delay fatigue and attenuate muscle necrosis, inflammation, and oxidative stress. Further evidence in favor of the PBM comes from an important study by Sussai et al., in which the application of LLLT immediately after swimming to exhaustion attenuated the number of apoptotic muscle cells. To our knowledge, only the study by Sussai et al. has examined the repercussion of LLLT on aerobic exercise–induced apoptosis and there is no information on whether a similar response could be obtained upon RE. Moreover, it is unclear how different doses of LLLT affect exercise-induced apoptosis. With this perspective in mind, we investigated whether LLLT, performed with three differences pre-exercise doses, would affect RE-induced muscle apoptosis in rats.
关键词： Resistance exercise,Dose-dependent,Photobiomodulation,Low-level laser therapy,Muscle apoptosis
更新于2025-09-19 17:13:59
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Does the combination of photobiomodulation therapy (PBMT) and static magnetic fields (sMF) potentiate the effects of aerobic endurance training and decrease the loss of performance during detraining? A randomised, triple-blinded,??placebo-controlled trial
摘要： Background: Photobiomodulation (PBMT) is a therapy that uses non-ionising forms of light, including low-level lasers and light-emitting diodes (LEDs) that may be capable of modulating cellular activity. Some biological processes may also interact with static magnetic fields (sMF), leading to modulatory effects on cells. Previous studies have verified that the combination of PBMT and sMF (PBMT/sMF) enhances the performance of individuals during aerobic training programs. The detraining period can cause losses in aerobic capacity. However, there is no evidence of the existence of any recourse that can decrease the effects of detraining. We aimed to investigate the effects of PBMT/sMF application during training and detraining to assess the effectiveness of this treatment in reducing the effects of detraining.
关键词： Light-emitting diode therapy,Endurance exercise,Low-level laser therapy,Phototherapy,Deconditioning
更新于2025-09-19 17:13:59
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Physical exercise and low-level laser therapy on the nociception and leukocyte migration of Wistar rats submitted to a model of rheumatoid arthritis
摘要： Rheumatoid arthritis denotes hyperplasia and intense inflammatory process. Treatment involves exercise protocols and use of resources such as low-level laser therapy (LLLT) to modulate the inflammatory process and maintain physical capacity. The objective was to investigate whether treatment with LLLT and exercise modulates the inflammatory process and peripheral functionality. Sample is composed of 128 male rats, separated into three groups, control, treated and untreated, in the acute and chronic period of the disease with 64 animals in each group, divided into 8 subgroups with n = 8. The animals were immunized with injection at the base of the tail and 7 days after intra-articular injection with complete Freund adjuvant (CFA) for lesion groups, and saline solution for the controls. Joint disability was evaluated by PET (paw elevation time) and joint edema and treated with LLLT and/or resisted stair climbing exercise. Normality Shapiro-Wilk test, ANOVA mixed for the functional analyses, and ANOVA one-way for the variables of cellular differentiation, with Bonferroni post hoc, p = 5% were used. For the evaluations of joint disability and nociception, there was a significant difference between the evaluations, the groups, and the interaction groups-evaluations. The treated groups showed recovery of functionality; it is still verified that laser therapy increased the nociceptive threshold of the chronic inflammatory period, and the exercise reflected in significant functional improvement and modulation of the inflammatory process both in the acute and chronic periods. LLLT, resistance exercise, or a combination of treatments had a positive effect on the modulation of the inflammatory process, reducing the migration of leukocytes, in addition to helping the return of peripheral functionality by reducing joint disability in a model of rheumatoid arthritis induced by CFA in rats.
关键词： Neurogenic inflammation,Rheumatoid arthritis,Laser therapy,Exercise,Leukocyte count
更新于2025-09-16 10:30:52
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The effect of low level laser irradiation on oxidative stress, muscle damage and function following neuromuscular electrical stimulation. A double blind, randomised, crossover trial
摘要： Background: Low level laser therapy (LLLT) is among novel methods for preventing and treating muscle damage and soreness induced by volitional exercise, but little is known about using LLLT before neuromuscular electrical stimulation. The aim of this first randomised, double blind, crossover trial addressing this issue was to evaluate effects of LLLT on muscle damage and oxidative stress, as well as recovery of muscle function after a single session of isometric neuromuscular electrical stimulation(NMES). Methods: Twenty four moderately active, healthy men aged 21–22 years received 45 electrically evoked tetanic, isometric contractions of the quadriceps femoris, preceded by LLLT or sham-LLLT. Maximal isometric voluntary muscle torques, perceived soreness, and blood samples were analysed from baseline to 96 h post intervention. We measured plasma markers of muscle damage (the activity of creatine kinase), and inflammation (C-reactive protein), and evaluated redox state parameters. Results: NMES-evoked contractions induced oxidative stress, demonstrated by an increase in lipid peroxidation and impairments in enzymatic antioxidant system. LLLT irradiations had a protective effect on NMES-induced decrease in enzymatic antioxidant defence and shortened the duration of inflammation. This effect of irradiations on redox state and inflammation did not affect lipid peroxidation, muscle damage, and muscle torque. Conclusions: LLLT may protect from impairments in enzymatic antioxidant system and may shorten inflammation induced by a single NMES session in moderately active, healthy men. However, the effects of LLLT on redox state and inflammatory processes do not seem to affect muscle damage and recovery of muscle function after NMES.
关键词： Photobiomodulation,Evoked contractions,Knee extensors,DOMS,Biochemistry,Exercise-induced muscle damage
更新于2025-09-16 10:30:52
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Efficacy of laser therapy for exercise-induced fatigue
摘要： Background: Laser therapy is widely used for exercise-induced fatigue, while the effect among different studies remains controversial. The present study was to summary available randomized controlled trials (RCTs) to evaluate the effect of laser therapy in subjects with exercise-induced fatigue. Methods: PubMed, Embase, and Cochrane Library were searched to identify the potential RCTs from inception to October 2017. The weighted mean difference (WMD) with 95% confidence intervals (CIs) was calculated using a random-effects model. Results: Twenty RCTs involving a total of 394 individuals were included in final analysis. No significant differences were observed between the laser therapy and control for the outcomes of lactate (WMD: (cid:1)0.19; 95%CI: (cid:1)0.52 to 0.13; P = .244), repetitions (WMD: 4.44; 95%CI: (cid:1)1.43 to 10.32; P = .138), work load (WMD: 3.38; 95%CI: (cid:1)1.15 to 7.91; P = .144), time taken to perform the exercise tests (WMD: 4.42; 95%CI: (cid:1)2.33 to 11.17; P = .199), creatine kinase (WMD: (cid:1)41.80; 95%CI: (cid:1)168.78 to 85.17; P = .519), maximum voluntary contraction (WMD: 23.83; 95%CI: (cid:1)7.41 to 55.07; P = .135), mean peak forces (WMD: 2.87; 95%CI: (cid:1)1.01 to 6.76; P = = .147), and visual analog scale (VAS) (WMD: (cid:1)1.91; 95%CI: (cid:1)42.89 to 39.08; P = = .927). The results of sensitivity analysis suggested that laser therapy might play an important role on the levels of lactate (WMD: (cid:1)0.30; 95%CI: (cid:1)0.59 to (cid:1)0.01; P = = .040), maximum voluntary contraction (WMD: 33.54; 95%CI: 1.95 to 65.12; P = = .037), and VAS (WMD: (cid:1)21.00; 95%CI: (cid:1)40.78 to (cid:1)1.22; P = = .037). The results of subgroup analyses indicated no significant differences between the laser therapy and placebo for lactate and repetitions when stratified by study design, mean age, gender, and study quality. Conclusions: The findings of this meta-analysis did not indicate any significant differences between the laser therapy and placebo.
关键词： meta-analysis,laser therapy,exercise-induced fatigue
更新于2025-09-12 10:27:22
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Comparison of laser speckle contrast imaging and laser-Doppler fluxmetry in boys and men
摘要： Objective We compare microvascular reactivity assessed by laser-Doppler fluxmetry (LDF) and laser speckle contrast imaging (LSCI) of boys and men during rest, post-occlusive reactive hyperaemia (PORH), and cycling exercise. Methods 19 boys (9±1 y) and 18 men (22±2 y) participated. LDF and LSCI measures were taken of the forearm during rest, PORH, and exercise. Results For all 3 assessments, the LSCI presented with higher flux values than the LDF for both boys and men (p<0.001). Bland-Altman analyses indicated that there was a positive linear bias between LSCI and LDF measurements in both boys and men. Regression analyses showed that the responses for the two methods were variable, depending on the particular assessment. For instance, at rest in boys there was no relationship between LDF and LSCI (r2=0.002), whilst in men there was a strong relationship (r2=0.86). Conclusions LSCI presented with higher values than LDF during rest, PORH, and exercise; the disparity between the two measures was larger as blood flow increased. The assessments were generally consistent, both methods appear to provide usable data for the assessment of microvascular reactivity in both boys and men. There are biases to each method and the data are not interchangeable between LDF and LSCI.
关键词： reactive hyperaemia,exercise,local control,Skin blood flow,paediatric
更新于2025-09-11 14:15:04
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Infrared Low-Level Laser Therapy (Photobiomodulation Therapy) before Intense Progressive Running Test of High-Level Soccer Players: Effects on Functional, Muscle Damage, Inflammatory, and Oxidative Stress Markers—A Randomized Controlled Trial
摘要： The effects of preexercise photobiomodulation therapy (PBMT) to enhance performance, accelerate recovery, and attenuate exercise-induced oxidative stress were still not fully investigated, especially in high-level athletes. The aim of this study was to evaluate the effects of PBMT (using infrared low-level laser therapy) applied before a progressive running test on functional aspects, muscle damage, and inflammatory and oxidative stress markers in high-level soccer players. A randomized, triple-blind, placebo-controlled crossover trial was performed. Twenty-two high-level male soccer players from the same team were recruited and treated with active PBMT and placebo. The order of interventions was randomized. Immediately after the application of active PBMT or placebo, the volunteers performed a standardized high-intensity progressive running test (ergospirometry test) until exhaustion. We analyzed rates of oxygen uptake (VO2 max), time until exhaustion, and aerobic and anaerobic threshold during the intense progressive running test. Creatine kinase (CK) and lactate dehydrogenase (LDH) activities, levels of interleukin-1β (IL-1-β), interleukin-6 (IL-6), and tumor necrosis factor alpha (TNF-α), levels of thiobarbituric acid (TBARS) and carbonylated proteins, and catalase (CAT) and superoxide dismutase (SOD) activities were measured before and five minutes after the end of the test. PBMT increased the VO2 max (both relative and absolute values—p < 0:0467 and p < 0:0013, respectively), time until exhaustion (p < 0:0043), time (p < 0:0007) and volume (p < 0:0355) in which anaerobic threshold happened, and volume in which aerobic threshold happened (p < 0:0068). Moreover, PBMT decreased CK (p < 0:0001) and LDH (p < 0:0001) activities. Regarding the cytokines, PBMT decreased only IL-6 (p < 0:0001). Finally, PBMT decreased TBARS (p < 0:0001) and carbonylated protein levels (p < 0:01) and increased SOD (p < 0:0001)and CAT (p < 0:0001) activities. The findings of this study demonstrate that preexercise PBMT acts on different functional aspects and biochemical markers. Moreover, preexercise PBMT seems to play an important antioxidant effect, decreasing exercise-induced oxidative stress and consequently enhancing athletic performance and improving postexercise recovery. This trial is registered with Clinicaltrials.gov NCT03803956.
关键词： PBMT,inflammatory markers,oxidative stress,Photobiomodulation therapy,infrared low-level laser therapy,exercise performance,muscle damage,soccer players
更新于2025-09-11 14:15:04
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[IEEE 2018 40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) - Honolulu, HI (2018.7.18-2018.7.21)] 2018 40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) - Robust Heart Rate Estimation During Physical Exercise Using Photoplethysmographic Signals
摘要： A method for estimating heart rate (HR) from photoplethysmographic (PPG) signal, during physical exercise, is presented in this paper. Accurate and reliable estimation of HR from PPG during intensive physical activity is challenging because intense motion artifacts can easily mask the true HR. If PPG signal is contaminated by intense motion artifacts, the highest peak of PPG spectrum is shifted from true HR due to motion artifacts. The proposed method employs a simple technique using spectral estimation and median filtering for HR estimation from intensely motion artifacts corrupted PPG signal. Experimental result for a database of 12 subjects recorded during fast running showed that the average absolute estimation error was 1.31 beats/minute.
关键词： spectral estimation,heart rate estimation,photoplethysmographic signals,motion artifacts,median filtering,physical exercise
更新于2025-09-09 09:28:46