- 标题
- 摘要
- 关键词
- 实验方案
- 产品
-
NanoJ: a high-performance open-source super-resolution microscopy toolbox
摘要： Super-resolution microscopy has become essential for the study of nanoscale biological processes. This type of imaging often requires the use of specialised image analysis tools to process a large volume of recorded data and extract quantitative information. In recent years, our team has built an open-source image analysis framework for super-resolution microscopy designed to combine high performance and ease of use. We named it NanoJ - a reference to the popular ImageJ software it was developed for. In this paper, we highlight the current capabilities of NanoJ for several essential processing steps: spatio-temporal alignment of raw data (NanoJ-Core), super-resolution image reconstruction (NanoJ-SRRF), image quality assessment (NanoJSQUIRREL), structural modelling (NanoJ-VirusMapper) and control of the sample environment (NanoJ-Fluidics). We expect to expand NanoJ in the future through the development of new tools designed to improve quantitative data analysis and measure the reliability of fluorescent microscopy studies.
关键词： Virus,Vaccinia,Archaea,Quantitative imaging,Sulfolobus acidocaldarius,Super-resolution microscopy,Fluidics,Modelling,Resolution,Image quality assessment,Pox,Image analysis,ImageJ,Fiji
更新于2025-09-19 17:15:36
-
[IEEE 2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC) - Munich, Germany (2019.6.23-2019.6.27)] 2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC) - Femtosecond Laser Induced Surface Micro-Structure Building by Material Ejection and Ablation on Cu and Al
摘要： Theoretical and experimental approaches verifying the fluidic operation of a partially shielded digital microfluidics device are presented in this paper. This paper is motivated by recent demand from the synthetic biology community for electrowetting on dielectric (EWD) enabled in-droplet electroporation, but is generalizable to a range of EWD applications that require shielding structures to be patterned on the EWD. An electrode patterned in an additional metal layer on the insulator that supports EWD actuation reduces the effective strength of the EW force due to dielectric shielding at the droplet contact line. A numerical model was developed to predict the impact of the partially shielding electrode on threshold voltage, EW force, fluid velocity, and droplet transport time. Compared with a batch of devices lacking the extra electrode, the presence of the added metal layer resulted in a 29% increase in threshold voltage, an 82% increase in transport time, and a 44% decrease in average transport velocity. Each trend agrees with the simulation results obtained from the fluid transport model. These results support the development of design rules for microfluidic devices that require partially shielding metal layers to integrate with EWD device architectures.
关键词： gene transfer,Fluidics,model,transport,electroporation,electrowetting on dielectric,electrotransfer,digital microfluidics,mechanics,finite element model,device
更新于2025-09-19 17:13:59