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Fluorescence imaging of invasive head and neck carcinoma cells with integrin αvβ6-targeting RGD-peptides: an approach to a fluorescence-assisted intraoperative cytological assessment of bony resection margins
摘要： We assessed the use of peptides containing arginylglycylaspartic acid (RGD) that target integrin (cid:2)v(cid:3)6 as a potential approach for a fluorescence-assisted intraoperative cytological assessment of bony resection margins (F-AICAB) in patients who had bone-infiltrating squamous cell carcinoma (SCC) of the head and neck. This was assessed to demarcate invasive carcinoma cells that stained for (cid:2)v(cid:3)6. Specimens from bony resection margins (n=362) were defined as either malignant or benign according to the results of cytological and histological examinations. Integrin (cid:2)v(cid:3)6-targeting fluorescence-labelled RGD peptides were added to the cytological samples and the accuracy of the resulting signal assessed by comparing it with the cytological findings. The value of F-AICAB was evaluated to find out if it could help to improve future diagnoses, tests, and treatments. Integrin (cid:2)v(cid:3)6 was strongly expressed in invasive SCC cells and qualified as a marker for bone-infiltrating carcinoma cells. It showed a high affinity to bind to invasive SCC cells and enabled swift and specific demarcation of (cid:2)v(cid:3)6-stained carcinoma cells. It was also diagnostic, with a sensitivity of 100% (95% CI 81.3% to 99.3%), specificity of 98.3% (95% CI 94.4% to 99.0%), positive predictive value of 92% (95% CI 70.2% to 94.3%), and negative predictive value of 100% (95% CI 96.9% to 99.9%), compared with the cytological findings. The targeting of specific integrin subtypes with selective, synthetic ligands, adapted for multimodal imaging, is a promising new approach to diagnosis. Further studies are necessary to provide more evidence for successful clinical translation and to establish the impact on clinical procedures.
关键词： Fluorescence imaging,Integrins,(cid:2)v(cid:3)6 integrin,Head and neck squamous cell carcinoma,RGD peptide,Cytology,Intraoperative margin control
更新于2025-09-04 15:30:14
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Feasibility of indocyanine green fluorescence imaging for intraoperative identification of parathyroid glands during thyroid surgery
摘要： Background: This study assessed the feasibility of near-infrared fluorescence imaging with indocyanine green (ICG) to identify the parathyroid glands (PGs) intraoperatively and to assess their perfusion after thyroid resection. Methods: Patients undergoing elective thyroidectomy were enrolled in this prospective study. An intravenous bolus of 7.5 mg ICG was administered twice: the first bolus to identify the PGs before resection of the thyroid and the second to assess vascularization of the PGs after resection. Results: A total of 30 operations in 26 patients were included. In 17 surgeries (56.7%), fluorescence imaging was of added value, especially to confirm the presence of a suspected PG. No intraoperative or postoperative complications occurred because of the use of ICG. Conclusion: Near-infrared fluorescence imaging with the use of ICG for intraoperative identification of the PGs and the assessment of its vascularization is feasible and safe and can provide more certainty about the location of the PGs.
关键词： near-infrared fluorescence imaging,thyroidectomy,NIRF,indocyanine green,parathyroid gland
更新于2025-09-04 15:30:14
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Far‐Red/Near‐Infrared Emissive (1,3‐Dimethyl)barbituric Acid‐Based AIEgens for High‐Contrast Detection of Metastatic Tumors in Lung
摘要： Despite of the enthusiastic research in aggregation-induced emission luminogens (AIEgens) in recent years, the ones that can be smoothly used for sophisticated biomedical applications such as in vivo bioimaging of pulmonary metastatic tumors during surgery are still limited. Herein, we report the design and synthesis of a new series of fluorescent AIEgens that consist of methoxy-substituted tetraphenylethene (TPE) as the electron-donating moiety, (1,3-dimethyl)barbituric acid as the electron-withdrawing moiety, and different π-bridge units. As compared to benzene or 3,4-ethylenedioxythiophene, using thiophene as the π-conjugation unit between the donor and acceptor results in relatively higher absolute fluorescence quantum yield (14.5%) in water when formulating the corresponding AIEgens into nanoparticles (AIE dots) with amphiphilic co-polymer as the doping matrix. The highly FR/NIR emissive thiophene-based AIE dots are demonstrated to be potent for intraoperative detection of pulmonary metastatic tumors particularly the micro-sized ones with excellent signal-to-background ratio.
关键词： barbituric acid,AIE dot,fluorescence imaging,image-guided cancer surgery,aggregation-induced emission luminogen
更新于2025-09-04 15:30:14
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Surgical management of proximal coronary arteriovenous fistulas using intraoperative fluorescence imaging
摘要： We report the use of intraoperative fluorescence imaging to assist in the surgical management of proximal coronary arteriovenous fistulas in four patients.
关键词： coronary arteriovenous fistula,fluorescence imaging,surgical technique
更新于2025-09-04 15:30:14