Fluorescence-Guided Cancer Diagnosis and Surgery by a Zero Cross-Talk Ratiometric Near-Infrared γ-Glutamytranspeptidase Fluorescent Probe

摘要： The ability to early detect cancer in an accurate and rapid fashion is of critical importance for cancer diagnosis and accurate resection in surgery. γ-Glutamyltranspeptidase (GGT) is overexpressed in several human cancers while keeps low expression in normal microenvironment, and thus recognized as an important cancer biomarker. To date, rational design of a zero cross-talk ratiometric near-infrared (NIR) GGT fluorescent probe for efficiently cancer diagnosis in various biological samples is still a big challenge. In this work, a zero cross-talk ratiometric NIR GGT fluorescent probe named Cy-GSH is developed. Cy-GSH shows highly sensitive to GGT, which is desired for cancer early diagnosis. Upon additional GGT, a large emission shift from 805 nm to 640 nm is observed, which is suitable for visualizing deeply located cancer in vivo. In addition, successful monitoring GGT activity in bloods, cells, tissues and in vivo, makes Cy-GSH possess great potentials for the clinical cancer early diagnosis. Furthermore, accurate visualizing tumors and metastases in mouse models illuminates that the probe may be a convenient tool for fluorescence-guided cancer surgery. To our knowledge, this is the first report to describe the strategy of a zero cross-talk ratiometric NIR GGT fluorescent probe for cancer early diagnosis and fluorescence-guided surgery.