- 标题
- 摘要
- 关键词
- 实验方案
- 产品
-
Fluorescence-based high throughput screening technologies for natural chloride ion channel blockers
摘要： Chloride channels represent a group of potential drug targets, their blockers showed significant protecting effect on impaired cells by modulating apoptosis, autophagy and other cell signals. However, clinical drugs with chloride channel inhibitory properties have not yet been developed. Natural product extract becomes an underlying candidate satisfied the clinical requirements for its low toxicity, low cost and abundant sources. Here, a fluorescence-based EYFP-H148Q/I153L-HeLa cell line model was constructed by molecular cloning and verified by Real-time PCR and Western Blotting assay. By using this chloride channel blocker screening model, 7 hit compounds chosen from 6988 natural compounds showed the channel blocking activity. Then the hit compounds were further validated by electrophysiological patch-clamp analysis. Our study preliminarily identified PC-4 as a new chloride channel inhibitors and demonstrated the reliability and sensitivity of fluorescence-based high throughput screening technologies for discovery of biologically active compounds from natural herbal compounds.
关键词： natural compound,high throughput screening,chloride channel blocker,patch clamp
更新于2025-09-23 15:21:21
-
Luminescent nanomaterials for droplet tracking in a microfluidic trapping array
摘要： The use of high-throughput multiplexed screening platforms has attracted significant interest in the field of on-site disease detection and diagnostics for their capability to simultaneously interrogate single-cell responses across different populations. However, many of the current approaches are limited by the spectral overlap between tracking materials (e.g., organic dyes) and commonly used fluorophores/biochemical stains, thus restraining their applications in multiplexed studies. This work demonstrates that the downconversion emission spectra offered by rare earth (RE)-doped β-hexagonal NaYF4 nanoparticles (NPs) can be exploited to address this spectral overlap issue. Compared to organic dyes and other tracking materials where the excitation and emission is separated by tens of nanometers, RE elements have a large gap between excitation and emission which results in their spectral independence from the organic dyes. As a proof of concept, two differently doped NaYF4 NPs (europium: Eu3+, and terbium: Tb3+) were employed on a fluorescent microscopy-based droplet microfluidic trapping array to test their feasibility as spectrally independent droplet trackers. The luminescence tracking properties of Eu3+-doped (red emission) and Tb3+-doped (green emission) NPs were successfully characterized by co-encapsulating with genetically modified cancer cell lines expressing green or red fluorescent proteins (GFP and RFP) in addition to a mixed population of live and dead cells stained with ethidium homodimer. Detailed quantification of the luminescent and fluorescent signals was performed to confirm no overlap between each of the NPs and between NPs and cells. Thus, the spectral independence of Eu3+-doped and Tb3+-doped NPs with each other and with common fluorophores highlights the potential application of this novel technique in multiplexed systems, where many such luminescent NPs (other doped and co-doped NPs) can be used to simultaneously track different input conditions on the same platform.
关键词： Rare earth elements,Single-cell analysis,Nanoparticles,Microfluidics,high-throughput screening
更新于2025-09-23 15:21:21
-
Validation of spectrophotometric determination of chlorogenic acid in fermentation broth and fruits
摘要： A fast and accurate ultraviolet-visible (UV-vis) spectrophotometric method was established to determine the presence of chlorogenic acid (CGA) according to potassium ferricyanide-Fe (III) detection system. The reaction temperature and pH level greatly influenced the CGA absorption spectrum. Maximum UV-vis absorption wavelength occurred at 790 nm under the optimum conditions (at 25 ℃ and pH 7.0). The results of UV-vis were further assessed by comparing the results with those of high-performance liquid chromatography (HPLC). The proposed method showed a wide linear sensing range of 10.0-800.0 μg mL-1 (R2 = 0.9996) and a high degree of precision (%R.S.D. < 1.50) and recovery (%R.S.D. > 3.39). Results of CGA and HPLC methods correlated well with each other. The proposed accurate, rapid, sensitive, low-cost, and high-throughput method was successfully used to quantify CGA in fermentation broth and fruits (Mango). Therefore, it may be applied for measuring CGA in biological samples.
关键词： Ultraviolet-visible spectrum,Chlorogenic acid,biological samples,high-throughput,HPLC
更新于2025-09-23 15:21:21
-
[IEEE 2019 26th IEEE International Conference on Electronics, Circuits and Systems (ICECS) - Genoa, Italy (2019.11.27-2019.11.29)] 2019 26th IEEE International Conference on Electronics, Circuits and Systems (ICECS) - Variable-Rate FEC Decoder VLSI Architecture for 400G Rate-Adaptive Optical Communication
摘要： Optical communication systems rely on forward error correction (FEC) to decrease the error rate of the received data. Since the properties of the optical channel will vary over time, a variable FEC coding gain would be useful. For example, if the channel conditions are benign, lower code overhead can be used, effectively increasing the code rate. We introduce a variable-rate FEC decoder architecture that can operate in several different modes, where each mode is linked to code rate and decoding iterations. We demonstrate a decoder implementation that provides a net coding gain range of 9.96–10.38 dB at a post-FEC bit-error rate of 10?15. For this range, a decoder implemented in a 28-nm process technology offers throughputs in excess of 400 Gbps, decoding latencies below 53 ns and a power dissipation of less than 0.95 W (or 1.3 pJ/information bit).
关键词： variable-rate FEC decoder,high-throughput,optical communication,VLSI architecture,coding gain
更新于2025-09-23 15:21:01
-
Optimisation of a high-throughput fluorescamine assay for detection of N-acyl-L-homoserine lactone acylase activity
摘要： N-acyl-L-homoserine lactone (AHL) acylases are a well-known group of enzymes that disrupt quorum sensing in Gram-negative bacteria by degrading AHL signalling molecules. This degradation of signalling molecules (termed 'quorum quenching') has potential uses in the prevention or reduction of biofilm formation and/or bacterial infections. Therefore, there is a great deal of interest in the identification and characterisation of quorum quenching enzymes. Here, we present an optimised fluorescamine-based assay for the detection of AHL acylase activity and demonstrate it can be used in a high-throughput screening format.
关键词： Fluorescamine,Quorum quenching,Fluorescence,High-throughput,AHL acylase
更新于2025-09-23 15:21:01
-
MALDI MS Imaging at Acquisition Rates Exceeding 100 Pixels per Second
摘要： The practicality of matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) applied to molecular imaging of biological tissues is limited by the analysis speed. Typically, a relatively low speed of stop-and-go micromotion of XY stages is considered as a factor substantially reducing the rate with which fresh sample material can be supplied to the laser spot. The sample scan rate in our laboratory-built high-throughput imaging TOF mass spectrometer was significantly improved through the use of a galvanometer-based optical scanner performing fast laser spot repositioning on a target plate. The optical system incorporated into the ion source of our MALDI TOF mass spectrometer allowed focusing the laser beam via a modified grid into a 10-μm round spot. This permitted the acquisition of high-resolution MS images with a well-defined pixel size at acquisition rates exceeding 100 pixel/s. The influence of selected parameters on the total MS imaging time is discussed. The new scanning technique was employed to display the distribution of an antitumor agent in 3D colorectal adenocarcinoma cell aggregates; a single MS image comprising 100 × 100 pixels with 10-μm lateral resolution was recorded in approximately 70 s.
关键词： MSI,TOF,Mass spectrometry imaging,High throughput,Laser beam scanning,Colorectal adenocarcinoma,3D cell aggregates,Spheroids,Grid ion source,MALDI
更新于2025-09-23 15:21:01
-
Thin silica-based microsheets with controlled geometry
摘要： A high demand for materials with defined geometry and size is required in a wide range of fields. Inorganic compounds, especially silica-based, arise as a cheap source and chemically flexible for the purpose. Silica display unique properties, like easy functionalization and good optical to manipulate. In this work, we developed a method to create thin silica microsheets with defined size and high-fidelity shape using superhydrophobic-hydrophilic microarrays. These microstructures were produced through sol-gel process using biomimetic superhydrophobic surfaces decorated with wettable spots. The results confirm the manufacture of porous silica microstructures with defined design (squares and circles) and thickness around 7 μm. The methodology applied in this work enables the high throughput fabrication of shaped silica materials in a single step, unlocking an extensive number of applications in areas that require miniaturization, like microelectronics or in fields like sensing and biomedicine.
关键词： Silica microsheets,High-fidelity shape,Inorganic materials,High throughput,Sol-gel process
更新于2025-09-23 15:19:57
-
Discovery of Novel Two-Dimensional Photovoltaic Materials Accelerated by Machine Learning
摘要： Searching for novel, high-performance, two-dimensional photovoltaic (2DPV) materials is an important pursuit for solar cell applications. In this work, an efficient method based on the machine learning algorithm combined with high-throughput screening is developed. Twenty-six 2DPV candidates are successfully ruled out from 187093 experimentally identified inorganic crystal structures, whose conversion efficiencies are predicted by density functional theory calculations. Our results indicate that Sb2Se2Te, Sb2Te3, and Bi2Se3 exhibit conversion efficiencies that are much higher than those of others, which make them promising 2DPV candidates for further applications. The superior photovoltaic performance is then analyzed, and the hidden structure-related relationships with photovoltaic properties are established, thus providing important information for the further examination of 2DPV materials. Given the rapid development of the database of materials, this approach not only provides an efficient way of searching for novel 2DPV materials but also can be applied to exploration of a broad range of functional materials.
关键词： high-throughput screening,machine learning,solar cell applications,density functional theory,two-dimensional photovoltaic materials
更新于2025-09-23 15:19:57
-
Use of BODIPY-Labeled ATP Analogues in the Development and Validation of a Fluorescence Polarization-Based Assay for Screening of Kinase Inhibitors
摘要： The screening of compound libraries to identify small-molecule modulators of specific biological targets is crucial in the process for the discovery of novel therapeutics and molecular probes. Considering the need for simple single-tool assay technologies with which one could monitor “all” kinases, we developed a fluorescence polarization (FP)-based assay to monitor the binding capabilities of protein kinases to ATP. We used BODIPY ATP-y-S as a probe to measure the shift in the polarization of a light beam when passed through the sample. We were able to optimize the assay using commercial Protein Kinase A (PKA) and H7 efficiently inhibited the binding of the probe when added to the reaction. Furthermore, we were able to employ the assay in a high-throughput fashion and validate the screening of a set of small molecules predicted to dock into the ATP-binding site of PKA. This will be useful to screen larger libraries of compounds that may target protein kinases by blocking ATP binding.
关键词： high-throughput screening,ATP-binding site,protein kinases,fluorescence polarization,BODIPY ATP-y-S
更新于2025-09-23 15:19:57
-
Higha??Throughput Characterization of Perovskite Solar Cells for Rapid Combinatorial Screening
摘要： In order to discover the ideal perovskite material for solar cell application, a large parameter space (composition, surrounding condition, fabrication technique etc.) must first be explored. Hence, screening this parameter space using a rapid combinatorial screening approach could drastically speed-up the rate of discovery. During the last decade, these discoveries and optimization processes of perovskite materials have been achieved using simple lab-scale deposition techniques and characterization methods, resulting in a substantial time-consuming process, slowing the rate of progress in field of photovoltaics. Thus, the benefits of developing fully-automated, high-throughput characterization techniques become apparent. In this paper, we detail a high-throughput solar cell testing system that enables parallel, real-time and comprehensive measurements, allowing for 16 solar cells to be characterized simultaneously. We show the importance of measurement reproducibility, condition verification and structured data post-processing.
关键词： Characterization,High-throughput,Perovskite Solar Cell
更新于2025-09-23 15:19:57