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A new lysosome-targetable fluorescent probe with a large Stokes shift for detection of endogenous hydrogen polysulfides in living cells
摘要： Hydrogen polysulfides (H2Sn, n>1) influence a variety of important biological functions and activities associated with hydrogen sulfide (H2S). The development of probes for rapid, selective, and sensitive detection of H2Sn still remains a great challenge. Lysosome plays crucial roles in various physiological processes among living cells, which arouse high interest in detecting endogenous lysosome-targetable H2Sn. To the best of our knowledge, there is no lysosome-targetable probe for H2Sn has been reported. In this work, a new lysosome-targetable probe NIPY-NF, based on the scaffold of imidazo[1,5-α]pyridine, with a large Stokes shift (215 nm), low detection limit (84 nM), fast response time (6 min) and low cytotoxicity was designed and synthesized. Furthermore, NIPY-NF was successfully applied into the cell imaging for detection of endogenous lysosome-targetable H2Sn.
关键词： Hydrogen polysulfides,Fluorescent probe,Lysosome,Cell imaging,Imidazo[1,5-α]pyridine
更新于2025-09-23 15:23:52
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A new turn-on fluorescent probe with ultra-large fluorescence enhancement for detection of hydrogen polysulfides based on dual quenching strategy
摘要： Based on dual quenching strategy (ESIPT inhibited quenching and PET quenching), we have developed a new turn-on fluorescent probe 1. Combining 3-(benzo[d]thiazol-2-yl)-10-butyl-10H-phenothiazin-2-ol (dye 2) as the fluorophore and 2-fluoro-5-nitro-benzoic as the recognition moiety, probe 1 had feature of notable large Stokes shift, highly sensitivity and selective for monitoring H2Sn with remarkable fluorescence enhancement (328-fold) response at 534 nm. Probe 1 exhibited excellent performance in the quantitative detection of H2Sn with a 137 nm Stokes shift and a low detection limit of 26 nM in solution. Finally, probe 1 was successfully utilized to image H2Sn in living A549 cells and zebrafish.
关键词： Large stokes shift,ESIPT,Hydrogen polysulfides,PET
更新于2025-09-19 17:15:36