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- 2017
- spectral reconstruction
- interference
- polarization
- transform
- imaging spectrometer
- Optoelectronic Information Science and Engineering
- BITTT
- Zhejiang University
- Guilin University of Aerospace Technology
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Evaluation of the temperature pattern of a complex body from thermal imaging and 3D information: A method and its MATLAB implementation
摘要： The standard setting of a camera used in Infrared thermography (IRT) is based on the choice of the same values of emissivity and distance for all pixels of a thermal image even if the emissivity depends on the relative position of camera and observed surface. Often this is not a problem. However, the resulting temperature pattern could be inadequate if a body having a complex shape is observed from strongly constrained positions. In order to face this issue, a procedure aimed at providing a correct temperature pattern by using 3D information related to a point cloud is proposed together with its MATLAB implementation (COMAP3 toolbox). For each pixel of a thermal image, the relative position of camera and observed surface is estimated, leading to pixel-specific values of emissivity and distance. The temperature obtained in this way is also mapped onto the point cloud. The effectiveness of the procedure in recognizing areas characterized by peculiar thermal behavior is shown in the case of a historic cylindrical masonry bell tower (Caorle’s bell tower, Venice, Italy).
关键词： Damage recognition,3D thermography,In situ measurements,Thermal imaging,Radiometric JPEG image
更新于2025-09-23 15:23:52
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Vibrational spectroscopic imaging and live cell video microscopy for studying differentiation of primary human alveolar epithelial cells
摘要： Alveolar type II(ATII) cells in the peripheral human lung spontaneously differentiate towards alveolar type I(ATI) cells, thus enabling air-blood barrier formation. Here, linear Raman and coherent anti-Stokes Raman scattering (CARS) microscopy are applied to study cell differentiation of freshly isolated ATII cells. The Raman spectra can successfully be correlated to gradual morphological and molecular changes during cell differentiation. Alveolar surfactant rich vesicles in ATII cells are identified based on phospholipid vibrations, while ATI-like cells are characterized by the absence of vesicular structures. Complementary, CARS microscopy allows for 3D visualization of lipid vesicles within ATII cells and their secretion, while hyperspectral CARS enables the distinction between cellular proteins and lipids according to their vibrational signatures. This study paves the path for further label-free investigations of lung cells and the role of the pulmonary surfactant, thus also providing a basis for rational development of future lung therapeutics.
关键词： confocal laser scanning microscopy,confocal Raman microscopy,pneumocyte type II differentiation,CARS microscopy,cell imaging
更新于2025-09-23 15:23:52
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The combination of Raman imaging and LIBS for quantification of original and degradation materials in Cultural Heritage
摘要： Quantification with nondestructive techniques is not very well developed in the field of Cultural Heritage despite its interest. In the last decades, several works have been published using Raman spectroscopy for quantifying. However, depending on the methodology used, the information provided by this technique is not complete, and the results could lead to misunderstanding when dealing with unknown samples. In this work, a novel double quantification using Raman imaging (its representativeness would be higher than point-by-point analysis) and Laser Induce Breakdown Spectroscopy (LIBS) analyses is presented for analysis of Cultural Heritage samples. Several dolomitic marble samples, with some calcite impurities, covered or not by a calcium oxalate (whewellite, CaC2O4·H2O) layer were analysed in order to optimize the novel methodology. The agreement between the quantitative results from the independent analyses of the Raman Image data and the LIBS data is consistent within the uncertainty arising from both techniques. Further, the same methodology was applied on the same samples but using point-by-point Raman analysis with portable instruments, and portable LIBS information, showing again a great agreement between them and with the results obtained using the laboratory instruments.
关键词： hydrated salts,quantification,Cultural Heritage,LIBS,Raman imaging
更新于2025-09-23 15:23:52
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Multi-fluorescent Cationic Carbon Dots for Solid-state Fingerprinting
摘要： Carbon nanomaterials have been widely explored in bioimaging and biosensing, but their application in forensic science and technology is rare. In this report, we show one-pot synthesis of cationic Carbon Dots (cCDs) and their application in fingerprinting analysis. The cCDs show narrow size distribution (<10 nm), decent quantum yield (c.a. 46%), and a band gap of 2.75 eV. The cCDs exhibit cationic charge and show excitation-dependent multi-fluorescence characteristics. The cCDs show no production of reactive oxygen species and thus are safe to use. Interestingly, they also possess bright liquid as well as solid-state fluorescence. We demonstrate that a well-resolved patterned fingerprint can be obtained within a short time (2-3 min) from different physical surfaces and can be studied using a simple cell phone camera enabling accurate visualization up to third-degree detailing of the fingerprint. Owing to multi-fluorescence characteristics of cCDs, a detailed investigation of the fluorescent patterns was carried out by a three-dimensional multiphoton confocal microscopy. In comparison to traditional methods, the cCDs fluorescence-based fingerprinting shows improvements towards rapid image capture and analysis. Thus, the cCDs form a promising material for rapid fingerprint detection in forensic sciences.
关键词： solid-state,confocal imaging,carbon dots,cell phone,fingerprint
更新于2025-09-23 15:23:52
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[Methods in Molecular Biology] Autophagy Volume 1880 (Methods and Protocols) || Correlative Light and Electron Microscopy of Autophagosomes
摘要： Live-cell imaging has been widely used to study autophagosome biogenesis and maturation. When combined with correlative electron microscopy, this approach can be extended to reveal ultrastructural details in three dimensions. The resolution of electron microscopy is needed when membrane contact sites and tubular connections between organelles are studied.
关键词： Autophagosome,Phagophore,Electron tomography,Live-cell imaging,Serial sectioning
更新于2025-09-23 15:23:52
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[Methods in Molecular Biology] T-Cell Motility Volume 1930 (Methods and Protocols) || Live Cell Imaging and Analysis to Capture T-Cell Motility in Real-Time
摘要： T-lymphocytes are the principle coordinators of the immune defense system and play a major role in the protection of our body against infections, intruders of non-self, and malignancies. To mount an immune response, T-cells need to be effectively employed to tissue sites of infection or in?ammation and establish contacts with antigen-presenting cells (APCs) or malignant cells. Understanding how T-cells navigate toward their recruitment sites would offer new therapeutic opportunities. Advancement in the hardware and software upgrades of microscopy technology has created several ef?cient and easy-to-operate live cell imaging platforms. In this protocol, we present a generalized and simple-to-follow protocol for live cell imaging of migrating T-cells, which can also be adopted to visualize real-time tracking of intracellular signaling events.
关键词： Advanced microscopy,Cell tracking,Live cell imaging,T-cell migration
更新于2025-09-23 15:23:52
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A touchless interaction interface for observing medical imaging
摘要： Using volume rendering to generate 3D models is associated with the problem of missing features on areas of interest, which are possibly concealed by other information. This article presents a novel focus-and-context medical imaging observation system using gesture-based technique to build a touchless interactive environment. The system offers two types of medical imaging observation tool, namely, 3D section cutting tool and 3-axes cross-section synchronization tool, enabling users to quickly and easily observe tissue sections. Feature classification was achieved using region growing and size-based transfer approaches. Combined with view penetration function (cylinder and cone view penetration functions), the system allows for direct observation of hidden features. The analytical experimental results verified that the proposed system is easy to operate in a touchless environment and creates positive user experience regarding observation and interaction.
关键词： Focus and context,Touchless,Medical imaging,Volume rendering,Visualization
更新于2025-09-23 15:23:52
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Optical Imaging of Triple-Negative Breast Cancer Cells in Xenograft Athymic Mice Using an ICAM-1-Targeting Small-Molecule Probe
摘要： Purpose: The development of early, accurate diagnostic strategies for triple-negative breast cancer (TNBC) remains a significant challenge. Intercellular adhesion molecule-1 (ICAM-1) overexpressed in human TNBC cells is a potential molecular target and biomarker for diagnosis. In this study, small-molecule probe (denoted as γ3-Cy5.5) constructed with a short 17-mer linear peptide (γ3) and near-infrared fluorescence (NIRF) dye cyanine 5.5 (Cy5.5) was used to detect the expression of ICAM-1 in vitro and in vivo, and to diagnose TNBC via NIRF imaging. Procedures: Western blotting and flow cytometric analysis were used for the detection of ICAM-1 expression in MDA-MB-231 and MCF-7 cells. The cytotoxicity of the small-molecule probe γ3-Cy5.5 was detected using the CCK8 assay. The in vitro targeting of the small-molecule probe γ3-Cy5.5 was verified via flow cytometry and a laser scanning confocal microscope. Finally, the targeting of small-molecule probe in vivo and ex vivo was observed by NIRF imaging. Results: Western blotting and flow cytometry demonstrate that ICAM-1 was highly expressed in the MDA-MB-231 TNBC cell line. Laser confocal microscopy and flow cytometry results show that TNBC cells have an increased cellular uptake of γ3-Cy5.5 compared to the control probe γ3S-Cy5.5. With in vivo NIRF, a significantly higher Cy5.5 signal appeared in the tumors of mice administered γ3-Cy5.5 than those treated with γ3S-Cy5.5. The target-to-background ratio observed on the NIRF images was significantly higher in the γ3-Cy5.5 group (10.2, 13.6) compared with the γ3S-Cy5.5 group (4.4, 4.0) at 1 and 2 h, respectively. Conclusions: This is the first report of the use of ICAM-1-specific small-molecule probe for in vivo NIRF optical imaging of TNBC. This method provides a noninvasive and specific strategy for the early diagnosis of TNBC.
关键词： Triple-negative breast cancer,Near-infrared fluorescence imaging,Small-molecule probe,ICAM-1
更新于2025-09-23 15:23:52
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PEGylated hollow gold nanoparticles for combined X-ray radiation and photothermal therapy in vitro and enhanced CT imaging in vivo
摘要： Up until now, hollow gold nanoparticles (HGNPs) with a spherical cavity have garnered much interest as theranostic agents in cancer therapy due to their high X-ray absorption and photothermal conversion ability. Herein, we describe the design of PEGylated hollow gold nanoparticles (mPEG@HGNPs) for combined X-ray radiation and photothermal therapy in vitro and enhanced computed tomography (CT) imaging in vivo using a breast tumor model. In vitro results revealed that mPEG@HGNPs could achieve a synergistic antitumor effect when irradiated by combined X-ray radiation and 808 nm near infrared laser light. Furthermore, mPEG@HGNPs exhibited a favorable tumor targeting effect and good CT contrast enhancement in both xenografted and orthotopic breast tumor models, due to the stealth effect of PEG which increased the enhanced permeability and retention (EPR) effect. These results suggest that mPEG@HGNPs may serve as multifunctional nanocomposites for cancer combination therapy and, thus, should be further studied.
关键词： hollow gold nanoparticles,CT imaging,PEGylated,combination therapy
更新于2025-09-23 15:23:52
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Near-Infrared Hyperspectral Imaging Rapidly Detects the Decay of Postharvest Strawberry Based on Water-Soluble Sugar Analysis
摘要： This paper presents a novel strategy to detect the fungal decay in strawberry using reflectance near-infrared hyperspectral imaging (NIR-HSI, 1000–2500 nm). The variation of fructose, glucose, sucrose, and total water-soluble sugar (TWSS) content was analyzed using HPLC with a reference method during fungal infection in strawberry. The feasibility of quantifying sugar constituents relevant to the different stages of decay in strawberry was evaluated using NIR-HSI with key wavelengths selected via successive projection algorithm. The results showed that the predicted performance of TWSS content was acceptable within 2 and 2.603 for RPD, respectively. Five to seven key wavelengths were obtained based on sugar constituents, and excellent performance for classification accuracy among the three stages of decay was 89.4 to 95.4% for calibration and 87.0 to 94.4% for prediction, respectively. This rapid approach provides a new strategy for the selection of key wavelengths to detect the decay and sugar constituents in strawberries.
关键词： Strawberry,Key wavelength,Decay,Sugar content,Hyperspectral imaging
更新于2025-09-23 15:23:52