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- 2017
- spectral reconstruction
- interference
- polarization
- transform
- imaging spectrometer
- Optoelectronic Information Science and Engineering
- BITTT
- Zhejiang University
- Guilin University of Aerospace Technology
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Multispectral optoacoustic tomography (MSOT) for imaging the particle size-dependent intratumoral distribution of polymeric micelles
摘要： Purpose: This study proposes the utilization of multispectral optoacoustic tomography (MSOT) to investigate the intratumoral distribution of polymeric micelles and effect of size on the biodistribution and antitumor efficacy (ATE). Materials and methods: Docetaxel and/or optoacoustic agent-loaded polymeric micelles (with diameters of 22, 48, and 124 nm) were prepared using amphiphilic block copolymers poly (ethylene glycol) methyl ether-block-poly (D,L lactide) (PEG2000–PDLLAx). Subcutaneous 4T1 tumor-bearing mice were monitored with MSOT imaging and IVIS? Spectrum in vivo live imaging after tail vein injection of micelles. The in vivo results and ex vivo confocal imaging results were then compared. Next, ATE of the three micelles was found and compared. Results: We found that MSOT imaging offers spatiotemporal and quantitative information on intratumoral distribution of micelles in living animals. All the polymeric micelles rapidly extravasated into tumor site after intravenous injection, but only the 22-nm micelle preferred to distribute into the inner tumor tissues, leading to a superior ATE than that of 48- and 124-nm micelles. Conclusion: This study demonstrated that MSOT is theranostically a powerful imaging modality, offering quantitative information on size-dependent spatiotemporal distribution patterns after the extravasation of nanomedicine from tumor blood vessels.
关键词： intratumoral distribution,MSOT,multispectral optoacoustic tomography,in vivo imaging,tumor model,particle size,polymeric micelle
更新于2025-09-23 15:23:52
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Imaging Patterns of Pediatric Pulmonary Blastomycosis
摘要： OBJECTIVE. The objective of our study was to characterize and update the radiologic patterns of pediatric pulmonary blastomycosis, and correlate the radiologic patterns with patient age. MATERIALS AND METHODS. Patients 0–18 years old with pulmonary blastomycosis who underwent chest imaging from 2005 to 2016 were included in this study. The following data were collected: age, sex, clinical information, and imaging findings including presence of extrapulmonary involvement and scarring on follow-up examinations. Concordance between radiography and CT was analyzed. RESULTS. Thirty-six patients (28 boys and 8 girls) ranging in age from 3 months to 17 years (mean, 10.5 years) were identified. Consolidation was found in 94.4% of patients and was unilateral in 76.5% of cases and bilateral in 23.5%. Upper (70.6%) and middle (47.1%) lobes were more frequently involved. Air bronchograms were identified in 76.5% of patients with consolidations, masslike consolidation was found in 55.9%, cavitation in 38.2%, and bubbly pattern (i.e., multiple small cavities) in 32.4%. In all patients younger than 5 years, consolidations involved multiple lobes. In 67.6% of patients, consolidations were associated with the following additional pulmonary or pleural abnormalities: pulmonary nodules (50% of patients), diffuse patchy opacification (26.5%), reticulonodular pattern (41.2%), atelectasis (5.9%), pleural effusion (20.6%), and hilar lymphadenopathy (23.5%). Pulmonary scarring was found in 70.4% of patients. Five patients had extrapulmonary involvement. The concordance between radiography and CT was excellent for location and extension of consolidation and diagnosis of cavitation, bubbly pattern, and nodules. CONCLUSION. The most common pattern of lung involvement from pulmonary blastomycosis in our series was a combination of consolidations with bilateral lung nodules and reticulonodular opacification.
关键词： chest radiography,imaging patterns,pediatric pulmonary blastomycosis,different age groups,chest CT
更新于2025-09-23 15:23:52
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Ethyl violet–bovine serum albumin fluorescent protein nanovessels target to lysosomes and mitochondria
摘要： Aim: Organelles are essential in maintaining homeostasis of mammalian cells. Monitoring the morphology and dynamics of organelles is of significance in cell state determination and disease diagnosis. Materials & methods: We describe here a new material called ethyl violet–bovine serum albumin fluorescent protein nanovessel (EV–BSA FPN). Upon heating, BSA was denatured to form higher polyhedral structures, which was prone to EV binding. These dye–protein hybrid materials were red fluorescence emissive upon excitation. Results: EV–BSA FPNs can be readily internalized by mammalian cells and dual localized in lysosomes and mitochondria. Besides, EV–BSA FPN can serve as carriers and efficiently deliver drug into cells. Conclusion: EV–BSA FPNs can be dual function fluorescent vessels for both dual-organelle imaging and drug delivery.
关键词： dual-organelle localization,fluorescent protein nanovessel,lysosomes and mitochondria imaging,ethyl violet,drug carrier
更新于2025-09-23 15:23:52
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[IEEE 2018 26th European Signal Processing Conference (EUSIPCO) - Rome (2018.9.3-2018.9.7)] 2018 26th European Signal Processing Conference (EUSIPCO) - Uncertainty Quantification in Imaging: When Convex Optimization Meets Bayesian Analysis
摘要： We propose to perform Bayesian uncertainty quantification via convex optimization tools (BUQO), in the context of high dimensional inverse problems. We quantify the uncertainty associated with particular structures appearing in the maximum a posteriori estimate, obtained from a log-concave Bayesian model. A hypothesis test is defined, where the null hypothesis represents the non-existence of the structure of interest in the true image. To determine if this null hypothesis is rejected, we use the data and prior knowledge. Computing such test in the context of imaging problem is often intractable due to the high dimensionality involved. In this work, we propose to leverage probability concentration phenomena and the underlying convex geometry to formulate the Bayesian hypothesis test as a convex minimization problem. This problem is subsequently solved using a proximal primal-dual algorithm. The proposed method is applied to astronomical radio-interferometric imaging.
关键词： astronomical imaging,proximal primal-dual algorithm,inverse problem,hypothesis testing,Bayesian uncertainty quantification
更新于2025-09-23 15:23:52
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Enhanced image processing and archiving capabilities of magneto-optical imaging for non-destructive evaluation
摘要： In its current state, the wide acceptance of the Magneto-Optical Imaging (MOI) technique is hindered due to noise, lack of recordable results, and impossibility of data post-processing. This paper presents some add-ons made to a commercial MOI system to ease the image interpretation, archiving and reporting of the results. In addition, a few image processing techniques are also employed in an attempt to perform automatic flaw detection. The recording capability of the MOI instrument output images was addressed by digitizing the video signal in video or image files. To help with the identification of the damage location and distance between images, a rotary quadrature encoder was mounted onto the MOI scan head. The use of the encoder allowed the identification of the inspection location with respect to a reference position, such as the beginning of the scan. Moreover, it allowed saving images at fixed intervals, which were then stitched into a single image, thus simplifying the post inspection analysis process. Both live and post-inspection image processing capabilities were made available. Implemented image processing included background subtraction, de-noising, contrast adjustment and morphological operation, among others. Contrast stretching transform and background subtractions were found to be among the most powerful techniques that could be used in simplifying the image interpretation.
关键词： non-destructive evaluation,Magneto-optical imaging,image processing
更新于2025-09-23 15:23:52
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Polydopamine-Based Tumor-Targeted Multifunctional Reagents for Computer Tomography/Fluorescence Dual-Mode Bioimaging-Guided Photothermal Therapy
摘要： Development of multifunctional diagnosis and treatment reagents is very meaningful in clinical application. Herein, we developed a polydopamine-based (PDA-based) tumor targeted multifunctional reagent by surface-initiated atom transfer radical polymerization (ATRP) strategy. First, the targeted PDA nanoparticles were prepared via combining with folic acid (FA) and dopamine. Then ATRP technology was used to graft the europium(III) complexes onto PDA surface (defined as FEDA). A series of detections revealed that the FEDA nanoparticles had been successfully prepared and exhibited a bright X-ray computer tomography (CT) and photoluminescence (PL) dual-mode imaging efficiency and an excellent photothermal therapy (PTT) effect in vivo/in vitro.
关键词： europium(III) complexes,polydopamine,dual-mode imaging-guided photothermal therapy,folic acid targeted,atom transfer radical polymerization
更新于2025-09-23 15:23:52
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A Fluorescent Cocktail Strategy for Differentiating Tumor, Inflammation, and Normal Cells by Detecting mRNA and H <sub/>2</sub> O <sub/>2</sub>
摘要： Accurately distinguishing tumors from noncancerous inflammation and normal tissues is hugely significant for tumor diagnosis and therapy. However, tumor and inflammatory tissues have similar pathologic characteristics in their microenvironment, making differentiation very difficult. Here, a fluorescent cocktail nanoparticle capable of simultaneously detecting intracellular mRNA and H2O2 was designed to differentiate tumors from nontumor cells. To detect targeted mRNA in living cells, a DNA probe was generated using the fluorescence resonance energy transfer (FRET) principle. A pH-responsive amphiphilic polymer was synthesized to realize the transportation of the DNA probe. In addition, the polymer was conjugated with a coumarin-boronic acid ester (Cou-BE) H2O2 probe. According to the change in the fluorescence of Cou-BE, tumor and inflammatory cells could be distinguished from normal cells owing to their high concentration of H2O2. Because of the different concentrations of tumor-related mRNA in tumor and nontumor cells, the fluorescence intensity of the DNA probe-loaded nanoparticles inside tumor cells was different from that inside inflammatory cells. Therefore, our fluorescent cocktail strategy could discriminate simultaneously tumor, inflammation, and normal cells through the cooperative detection of intracellular mRNA and H2O2, which demonstrated potential application value in biomedical research and clinical diagnosis.
关键词： H2O2,tumor,tumor-related mRNA,fluorescence imaging,inflammation
更新于2025-09-23 15:23:52
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Optical Imaging of Charges with Atomically Thin Molybdenum Disulfide
摘要： Mapping local surface charge distribution is critical to the understanding of various surface processes and also allows the detection of molecules binding to the surface. We show here that the optical absorption of monolayer MoS2 is highly sensitive to charge and demonstrate optical imaging of local surface charge distribution with this atomically thin material. We validate the imaging principle and perform charge sensitivity calibration with an electrochemical gate. We further show that binding of charged molecules to the atomically thin material leads to a large change in the image contrast, allowing determination of the charge of the adsorbed molecules. This capability opens possibilities for characterizing impurities and defects in two dimensional materials and for label-free optical detection and charge analysis of molecules.
关键词： electrochemical gate,monolayer molybdenum disulfide,protein binding,local charge imaging,charged impurities
更新于2025-09-23 15:23:52
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Characterizing a novel scintillating glass for application to megavoltage cone-beam computed tomography
摘要： Purpose: The purpose of the present study was to evaluate the performance of a prototype electric portal imaging device (EPID) with a high detective quantum efficiency (DQE) scintillator, LKH-5. Specifically, image quality in context of both planar and megavoltage (MV) cone-beam computed tomography (CBCT) is analyzed. Methods: Planar image quality in terms of modulation transfer function (MTF), noise power spectrum (NPS), and detective quantum efficiency (DQE) are measured and compared to an existing EPID (AS-1200) using the 6 MV beamline for a Varian TrueBeam linac. Imager performance is contextualized for three-dimensional (3D), MV-CBCT performance by measuring imager lag and analyzing the expected degradation of the DQE as a function of dose. Finally, comparisons between reconstructed images of the Catphan phantom in terms of qualitative quality and signal-difference-to-noise ratio (SDNR) are made for 6 MV images using both conventional and LKH-5 EPIDs as well as for the kilovoltage (kV) on-board imager (OBI). Results: Analysis of the NPS reveals linearity at all measured doses using the prototype LKH-5 detector. While the first zero of the MTF is much lower for the LKH-5 detector than the conventional EPID (0.6 cycles/mm vs. 1.6 cycles/mm), the normalized NPS (NNPS) multiplied by total quanta (qNNPS) of the LKH-5 detector is roughly a factor of 7-8 times lower, yielding a DQE(0) of approximately 8%. First, second, and third frame lag was measured at approximately 23%, 5%, and 1% respectively, although no noticeable image artifacts were apparent in reconstructed volumes. Analysis of low dose performance reveals that DQE(0) remains at 80% of its maximum value at a dose as low as 7.5x10-6 MU. For a 400 projection technique, this represents a total scan dose of 0.0030 MU, suggesting that if imaging doses are increased to a value typical of kV-CBCT scans (~2.7 cGy), the LKH-5 detector will retain quantum noise limited performance. Finally, comparing Catphan scans, the prototype detector exhibits much lower image noise than the conventional EPID, resulting in improved small object representation. Further, SDNR of H2O and polystyrene cylinders improved from -1.95 and 2.94 to -15 and 18.7, respectively. Conclusions: Imaging performance of the prototype LKH-5 detector was measured and analyzed for both planar and 3D contexts. Improving noise transfer of the detector results in concurrent improvement of DQE(0). For 3D imaging, temporal characteristics were adequate for artifact-free performance and at relevant doses, the detector retained quantum-noise limited performance. Although quantitative MTF measurements suggest poorer resolution, small object representation of the prototype imager is qualitatively improved over the conventional detector due to the measured reduction in noise.
关键词： EPID,NPS,scintillator,MV-CBCT,DQE,MTF,Portal imaging
更新于2025-09-23 15:23:52
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Harnessing the synergy between upconverting nanoparticles and lanthanide complexes in a multi-wavelength responsive hybrid system
摘要： We prepared a hybrid system composed of a continuous film of dinuclear lanthanide complex [Ln2bpm(tfaa)6] (Ln = Tb or Eu) and upconverting nanoparticles (UCNPs) using a straightforward drop-cast methodology. The system displayed visible emission under near-infrared (NIR) excitation, simultaneously stemming from sub-10-nm UCNPs and [Ln2] complexes, the latter species being otherwise directly excitable only using UV-blue radiation. In light of the results of steady-state – including power-dependent – and time-resolved optical measurements, we identified the radiative, primarily ligand-mediated nature of the energy transfer from Tm3+ ions in the UCNPs-to-Ln3+ ions in the complexes. Hyperspectral mapping and electron microscopy observations of the surface of the hybrid system confirmed the continuous and concomitant distribution of UCNPs and lanthanide complexes over the extensive composite films. Key features of the hybrid system are the simultaneous UV-blue and NIR light harvesting capabilities and their ease of preparation. These traits render the presented hybrid system a formidable candidate for the development of photoactivated devices capable to operate under multiple excitation wavelength and to transduce the absorbed light into narrow, well-defined spectral regions.
关键词： hybrid system,complex,energy transfer,lanthanide,films,upconverting nanoparticles,hyperspectral imaging
更新于2025-09-23 15:23:52