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Time-lapse imaging of sentinel lymph node using indocyanine green with near-infrared fluorescence imaging in early endometrial cancer
摘要： Objective: Indocyanine green with near-infrared fluorescence imaging (NIR-ICG) is a new tracer modality in the limelight used for lymphatic mapping. The advantage of this method is to provide real-time image during surgery. To use ICG for image guided lymph node dissection, a surgeon needs to know initial appearing time and duration. Methods: A 52-year-old woman undertook surgery diagnosed with endometrial cancer. She had no past medical history and her body mass index was 25.3 kg/m2. Preoperative magnetic resonance imaging examination revealed 2.7 cm sized cancerous mass in the endometrial cavity with superficial myometrial invasion without lymph node enlargement. Four mL (1.25 mg/mL) of ICG solution was prepared for injection. For each site, 1 mL of solution was injected superficially, 2–3 mm into the cervical submucosa and another 1 mL was injected deep, 1–2 cm into the stroma of the cervix [1,2]. We recorded video with 30° 10 mm scope equipped with a specific lens and light source emitting both visible and NIR light (KARL STORZ GmbH & Co. KG, Tuttlingen, Germany). Results: Pelvic lymph node was visualized from around 5 minutes. ICG was dispersed into organs after hysterectomy (53 minutes after ICG injection), yet we could clearly identify sentinel lymph node (SLN). Pathology revealed endometriod adenocarcinoma grade I, myometrial invasion with less than half of myometrium and no lymph node metastasis. Conclusion: Cervical injection of ICG provides good visualization of SLN from 5 minutes to over an hour. Our film gives an idea about time management to make a plan for surgery and not to miss SNLs.
关键词： Indocyanine Green,Laparoscopy,Lymph Nodes,Endometrial Neoplasms,Time Management,Fluorescence
更新于2025-09-10 09:29:36
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Transanal total mesorectal excision for rectal cancer with indocyanine green fluorescence angiography
摘要： Background The aim of this study was to evaluate the impact of fluorescence angiography (FA) on any change in proximal resection margin and/or anastomotic leak (AL) following transanal total mesorectal excision (TaTME) for rectal cancer (RC). Methods This retrospective cohort study was conducted at two centers by three senior surgeons. Both institutions’ prospectively maintained Institutional Review Board-approved databases were retrospectively queried for all consecutive patients between July 2015 and May 2017 who had laparoscopic hybrid trans-abdominal total mesorectal excision (TME) and TaTME for RC with colorectal or coloanal anastomosis < 10?cm from the anal verge. All patients had intraoperative FA to assess colonic perfusion of the planned proximal resection margin before bowel transection and after construction of the anastomosis. Primary outcomes measured any changes in proximal resection margins and AL rates. Results Fifty-four patients (31 males; mean age 63 ± 12?years) were included; 30 (55%) of whom received neoadjuvant chemoradiation. The average anastomotic height was 3.6?cm from the anal verge and 8 (14.5%) patients required intersphincteric dissection. Forty-six patients (85%) had loop ileostomy. FA led to a change in the proximal resection margin in 10 patients (18.5%), one of whom had AL on postoperative day 3 requiring diagnostic laparoscopy and loop ileostomy. A second patient, without a change in the proximal resection margin, also had an AL. The overall AL rate was 3.7%. Conclusions FA changed the planned proximal resection margin in 18.5% of patients, possibly accounting for the relatively low AL rate. FA is imperfect, and subjective but does have the potential to improve outcomes.
关键词： Transanal total mesorectal excision,Fluorescence angiography,Indocyanine green,Anastomotic leak,Rectal cancer,Resection margin
更新于2025-09-10 09:29:36
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Photoacoustic and fluorescent effects in multilayer plasmon-dye interfaces
摘要： Progress in understanding the cell biology and progression of disease depends on advanced imaging and labeling techniques. Here, we address this demand by exploring novel multilayered nanocomposites (MNCs) with plasmonic nanoparticles and adsorbing dyes in thin nonabsorbing shells as supercontrast multimodal photoacoustic (PA) and fluorescent agents in the near-infrared range. The proof of concept was performed with gold nanorods (GNRs) and indocyanine green (ICG) dispersed in a matrix of biodegradable polymers. We demonstrated synergetic PA effects in MNCs with the gold–ICG interface that could not be achieved with ICG and GNRs alone. We also observed ultrasharp PA and emission peaks that could be associated with nonlinear PA and spaser effects, respectively. Low-toxicity multimodal MNCs with unique plasmonic, thermal, and acoustic properties have the potential to make a breakthrough in PA flow cytometry and near-infrared spasers in vivo by using the synergetic interaction of plasmonic modes with a nearby absorbing medium.
关键词： gold nanorods,fluorescence,in vivo flow cytometry,biocompatible polymers,photoacoustic effect,indocyanine green,multilayer composite,fluorescence quenching
更新于2025-09-10 09:29:36
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Evaluation of graft anastomosis using time–intensity curves and quantitative near-infrared fluorescence angiography during peripheral arterial bypass grafting
摘要： Near-infrared fluorescence angiography (NIR) visualizes blood perfusion using the fluorescence property of indocyanine green (ICG). This study aimed to retrospectively determine the usefulness of a quantitative analysis using NIR to predict the patency of peripheral arterial bypass grafts by measuring their fluorescence luminance intensities (FLIs). Thirteen grafts in 11 patients who underwent peripheral arterial bypass grafting were divided into a patent graft group (n = 7) and a failed graft group (n = 6). The changes in the FLIs of ICG opacification through the graft and distal host artery were retrospectively analyzed using stored NIR data. The time–intensity curves (TICs) of ICG opacification through the graft (Qgraft) and distal host artery (Qdistal) were measured. Two parameters, Δ(Qgraft ? Qdistal) and integral(Qgraft ? Qdistal), were also analyzed. Although not significant, decreases in Qgraft were observed in the failed graft groups. The Qdistal of the failed graft group was significantly attenuated as compared with that of the patent graft group. Δ(Qgraft ? Qdistal) increased only in the failed graft group, which indicates widening of the gap in FLI. Integral(Qgraft ? Qdistal) was higher in the failed graft group, as it reflects the accumulation of ICG opacification. The TICs were influenced by anastomotic stenosis in the distal site of the host arteries. Our results indicate that the comparison of Δ(Qgraft ? Qdistal) and integral (Qgraft ? Qdistal) quantitatively analyzed using NIR can potentially predict anastomotic stenosis.
关键词： Indocyanine green,Near-infrared fluorescence angiography,Peripheral arterial bypass graft
更新于2025-09-10 09:29:36
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Application of a Fluorescence Imaging System with Indocyanine Green to Protect the Parathyroid Gland Intraoperatively and to Predict Postoperative Parathyroidism
摘要： Identifying the parathyroid gland during surgery may be difficult for surgeons. It is critical for them to be able to locate it during thyroidectomy or parathyroidectomy because of the significant function of the parathyroid in the calcium balance. According to recent reports, intrinsic fluorescence of the parathyroid has been found. There is some evidence to suggest that new equipment can detect the fluorescence via imaging technology. In this study, a newly invented intraoperative fluorescence imaging system and indocyanine green (ICG) dye were applied to detect the parathyroid glands and evaluate the vascularization of the parathyroid.
关键词： Postoperative parathyroidism,Indocyanine green,Parathyroid,Fluorescence imaging system
更新于2025-09-10 09:29:36
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Zinc(II)-dipicolylamine Coordination Nanotheranostics: Toward Synergistic Nanomedicine by Combined Photo/gene Therapy
摘要： We report a rational design for coordination-driven self-assembly metal-organic nanostructures as multifunctional nanotheranostics. The zinc(II) coordination-based nano-formulations capable of loading indocyanine green (ICG) and therapeutic genes were prepared to achieve a fluorescence/photoacoustic imaging guided photo/gene combinational therapy strategy. We showed the enhanced theranostic capability of zinc(II)-dipicolylamine-assisted assembly of ICG, as well as simultaneous targeted gene delivery in an experimental mouse model of cancer. Such a co-assembly strategy provides a facile way to achieve function hybridization ensuring a "win-win" cooperation in personalized nanomedicine.
关键词： Zn(II)-dipicolylamine,gene therapy,phototherapy,indocyanine green,metal-organic nanostructure
更新于2025-09-10 09:29:36
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[IEEE 2018 40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) - Honolulu, HI, USA (2018.7.18-2018.7.21)] 2018 40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) - Towards an ASIC-based Fluoroscopic Capsule for the Early Cancer Detection in the Small Intestine
摘要： Early micro-cancer detection in the small intestine can be realized using infrared fluorescence endoscopy (IRFE) in conjunction with an infrared fluorescence biomarker. In this paper, we present a third-generation capsule that detects weak fluorescence signals emitted by low concentrations of indocyanine green (ICG). An application-specific integrated circuit (ASIC) has been designed and fabricated that integrates many of the peripheral components of the capsule system. The ASIC enables the system to have greater sensitivity whilst reducing the capsule size and lowering the power consumption.
关键词： infrared fluorescence endoscopy,small intestine,indocyanine green,application-specific integrated circuit,early cancer detection
更新于2025-09-10 09:29:36
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Indocyanine Green labeling for optical and photoacoustic imaging of Mesenchymal Stem Cells after in vivo transplantation
摘要： The transplantation of Mesenchymal Stem Cells (MSCs) holds great promise for the treatment of a plethora of human diseases, but new non-invasive procedures are needed to monitor the cell fate in vivo. Already largely used in medical diagnostics, the fluorescent dye Indocyanine Green (ICG) is an established dye to track limited numbers of cells by optical imaging, but it can be visualized also by Photoacoustic Imaging (PAI), which provides a higher spatial resolution than pure near infrared fluorescence imaging (NIRF). Because of its successful use in clinical and preclinical examinations, we chose ICG as PAI cell labeling agent. Optimal incubation conditions were defined for an efficient and clinically translatable MSC labeling protocol, such that no cytotoxicity or alterations of the phenotypic profile were observed, and a consistent intracellular uptake of the molecule was achieved. Suspensions of ICG-labeled cells were both optically and optoacoustically detected in vitro, revealing a certain variability in the photoacoustic spectra acquired by varying the excitation wavelength from 680 to 970 nm. Intramuscular engraftments of ICG-labeled MSCs were clearly visualized by both PAI and NIRF over few days after transplantation in the hindlimb of healthy mice, suggesting that the proposed technique retains a considerable potential in the field of transplantation-focused research and therapy.
关键词： Indocyanine Green,Near Infrared Fluorescence Imaging,Photoacoustic Imaging,Cell tracking,Stem Cells
更新于2025-09-10 09:29:36
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The Application of FLOW 800 ICG Videoangiography Color Maps for Neurovascular Surgery and Intraoperative Decision Making
摘要： BACKGROUND: Indocyanine green (ICG) videoangiography can assess cerebral blood flow, but results are primarily qualitative. FLOW 800 software measures fluorescence dynamics and creates a semiquantitative color delay map for assessment of relative sequence of blood flow within the vasculature. METHODS: We retrospectively reviewed 23 consecutive patients for whom FLOW 800 ICG videoangiography was used. They harbored aneurysms, arteriovenous malformations (AVMs), dural arteriovenous fistula (dAVF), or hemangioblastoma. Patients’ characteristics, FLOW 800 data, and clinical findings were recorded. Color map data were readily available intraoperatively and guided surgery. RESULTS: The cohort included 10 patients with AVMs, 11 with aneurysms, 1 with dAVF, and 1 with hemangioblastoma. Approximately two thirds of patients underwent intraoperative angiography. FLOW 800 data provided semiquantitative data regarding localization, flow status in major feeding arteries, and dominance of the arterialized draining veins for AVMs, more than data from ICG videoangiography alone. For complex aneurysms, color maps confirmed relative adequate flow in parent and branching vessels. For the foramen magnum dAVF, the location of the dominant transdural connection was appreciated only via flow analysis. Flow analysis created the blood flow map of a large complex solid brainstem hemangioblastoma and guided devascularization. All FLOW 800 findings agreed with intraoperative and postoperative angiography. CONCLUSIONS: ICG videoangiography with FLOW 800 analysis can provide semiquantitative and relative flow magnitude data that are efficient and noninvasive. This process helps identify early arterialized veins and their flow status during AVM and dAVF surgery and can confirm adequate relative flow within branching vessels during aneurysm surgery when clip-induced stenosis is suspected.
关键词： Videoangiography,Dural arteriovenous fistula,Brain arteriovenous malformations,Indocyanine green,Ligation,FLOW 800,Cerebral aneurysm
更新于2025-09-10 09:29:36
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P2.16-27 Indocyanine Green Virtual Assisted Lung Mapping (ICG-VAL-MAP): Anyone Can Perform a Visible Preoperative Marking Successfully
摘要： We con?rmed that ICG-VAL-MAP was a novel and promising technique with better visibility for the complete resection of small pulmonary nodules.
关键词： Preoperative marking,3D-CT simulation,Indocyanine green
更新于2025-09-09 09:28:46