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Ab initio model for the chlorophyll-lutein exciton coupling in the LHCII complex
摘要： 2A_g^- state of lutein plays a crucial role in photoprotection of higher plants. Due to its multiconfigurational nature, accurate description of this electronic state and respective transition properties is a formidable task. In this paper, applicability of various CASSCF and RASSCF formulations for description of the 2A_g^- state is discussed. It is shown that inclusion of the entire π-system of lutein into the active space is required for accurate calculation of transition properties. Exciton coupling in the chlorophyll-lutein dimer involved in non-photochemical quenching in the LHCII complex was calculated to provide a connection between pigment interactions and non-photochemical quenching regulation.
关键词： non-photochemical quenching,MCSCF,Xanthophyll,lutein,exciton coupling,LHCII
更新于2025-09-23 15:23:52
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Fluorescence Microscopy of Single Liposomes with Incorporated Pigment–Proteins
摘要： Reconstitution of transmembrane proteins into liposomes is a widely used method to study their behavior under conditions closely resembling the natural ones. However, this approach does not allow precise control of the liposome size, reconstitution efficiency and the actual protein-to-lipid ratio in the formed proteoliposomes, which might be critical for some applications and/or interpretation of data acquired during the spectroscopic measurements. Here we present a novel strategy employing methods of proteoliposome preparation, fluorescent labelling, purification, and surface immobilization that allow us to quantify these properties using fluorescence microscopy at the single-liposome level and for the first time apply it to study photosynthetic pigment–protein complexes LHCII. We show that LHCII proteoliposome samples, even after purification with a density gradient, always contain a fraction of non-reconstituted protein and are extremely heterogeneous in both protein density and liposome sizes. This strategy enables quantitative analysis of the reconstitution efficiency of different protocols and precise fluorescence spectroscopic study of various transmembrane proteins in a controlled native-like environment.
关键词： Fluorescence Microscopy,Proteoliposomes,Pigment–Proteins,LHCII,Single Liposomes
更新于2025-09-23 15:21:01