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Precisely Encoded Barcodes Using Tetrapod CdSe/CdS Quantum Dots with a Large Stokes Shift for Multiplexed Detection
摘要： A serious obstacle to the construction of high-capacity optical barcodes in suspension array technology is energy transfer, which can prompt unpredictable barcode signals, limited barcode numbers, and the need for an unfeasible number of experimental iterations. This work reports an effective and simple way to eliminate energy transfer in multicolor quantum dots (QDs)-encoded microbeads by incorporating tetrapod CdSe/CdS QDs with a large Stokes shift (about 180 nm). Exploiting this unique feature enables the facile realization of a theoretical 7 × 7-1 barcoding matrix combining two colors and seven intensity levels. As such, microbeads containing tetrapod CdSe/CdS QDs are demonstrated to possess a powerful encoding capacity which allows for precise barcode design. The ability of the Shirasu porous glass membrane emulsification method to easily control microbead size facilitates the establishment of a 3D barcode library of 144 distinguishable barcodes, indicating the enormous potential to enable large-scale multiplexed detection. Moreover, when applied for the multiplexed detection of five common allergens, these barcodes exhibit superior detection performance (limit of detection: 0.01–0.02 IU mL?1) for both spiked and patient serum samples. Therefore, this new coding strategy helps to expand barcoding capacity while simultaneously reducing the technical and economic barriers to the optical encoding of microbeads for high-throughput multiplexed detection.
关键词： large Stokes shift,F?rster resonance energy transfer (FRET),photon re-absorption,quantum dots-encoded microbeads,multiplexed detection
更新于2025-09-11 14:15:04
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A multicolor large Stokes shift fluorogen-activating RNA aptamer with cationic chromophores
摘要： Large Stokes shift (LSS) fluorescent proteins (FPs) exploit excited state proton transfer pathways to enable fluorescence emission from the phenolate intermediate of 4-hydroxybenzylidene imidazolone (HBI) chromophore. An RNA aptamer named Chili mimics LSS FPs by inducing highly Stokes-shifted emission from several new green and red HBI analogs that are non-fluorescent when free in solution. The ligands are bound by the RNA in their protonated phenol form and feature a cationic aromatic side chain for increased RNA affinity and reduced magnesium dependence. In combination with oxidative functionalization at the C2 position of the imidazolone, this strategy yielded DMHBO+, which binds to the Chili aptamer with a low-nanomolar KD. Because of its highly red-shifted fluorescence emission at 592 nm, the Chili–DMHBO+ complex is an ideal fluorescence donor for F?rster resonance energy transfer (FRET) to the rhodamine dye Atto 590 and will therefore find applications in FRET-based analytical RNA systems.
关键词： RNA aptamer,fluorescence,fluorescence resonance energy transfer,fluorescent protein,large Stokes shift
更新于2025-09-10 09:29:36
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Highly sensitive fluorescent probe based on a novel phenothiazine dye for detection of thiophenols in real water samples and living cells
摘要： Based on an excited-state intramolecular proton transfer (ESIPT) fluorophore, a novel fluorescent off-on probe for detection of thiophenols was designed and synthesized. This probe (λex = 401 nm, λem = 527 nm) displayed high specificity for sensing thiophenols over other biologically related species. Besides, this probe possessed capabilities of monitoring thiophenols with rapid response rate (3 min), a large Stokes shift (126 nm), and high sensitivity (2.7 nM). The sensing mechanism was considered to be that thiophenols triggered thiolysis of the probe and the ESIPT fluorophore was released, as confirmed by means of HPLC and HRMS. Most notably, this probe was successfully applied to monitor levels of thiophenols in realistic samples and MDA-MB-231 cells.
关键词： Thiophenol,Large stokes shift,Probe,MDA-MB-231 cells
更新于2025-09-04 15:30:14
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Iminocoumarin-based red to near-infrared fluorescent turn-on probe with a large Stokes shift for imaging H2S in living cells and animals
摘要： The development of organic dye-based fluorescent probes for detection of H2S in living systems has attracted considerable attention in recent years. In this work, a novel tetrahydroquinoxaline iminocoumarin-based red to near-infrared (red-to-NIR) fluorescent probe was developed for detection of H2S. This probe shows a rapid and distinct red-to-NIR fluorescent turn-on detection process for H2S with high selectivity and sensitivity (the detection limit was determined to be as low as 10 nM at physiological pH). In addition, this probe exhibits a remarkable large Stokes shift (128 nm) and low cytotoxicity, and can be applied for imaging H2S in living cells and animals. All these results demonstrated that this new probe is promising for detection of H2S both in vitro and in vivo.
关键词： large Stokes shift,hydrogen sulfide,living cells and animals,near-infrared fluorescent probe,iminocoumarin
更新于2025-09-04 15:30:14