A Novel Chemiluminescent Probe Based on 1,2-Dioxetane Scaffold for Imaging Cysteine in Living Mice

摘要： A novel chemiluminescent probe for detection of cysteine (Cys) from other biothiols has been reported by utilizing the excellent chemiluminescent Schaap’s adamantylidene-dioxetane scaffold. After careful assessment, the probe CL-Cys could detect Cys with high sensitivity and total light photons increased with 28-fold after the probe was treated with Cys, with the detection limit of 7.5 x 10-8 M. Finally, CL-Cys was further utilized to the chemiluminescent imaging of endogenous Cys in living mouse. In general, this probe has a remarkable ability of detecting Cys, which provides a valuable method for interrogation the Cys roles in more biological and pathological processes.