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Ultrasensitive U-shaped fiber optic LSPR cytosensing for label-free and in situ evaluation of cell surface N-glycan expression
摘要： Label-free and in situ detection of N-glycan expression on surface of cells is vital for understanding the progression of cancer. Herein, cytosensing based on U-shaped fiber optic local surface plasmon resonance (LSPR) was developed for cancer cell detection and N-glycan evaluation on cell surface. The U-shaped fiber optic LSPR has high refractive index sensitivity (RIS). Therefore, U-shaped fiber optic LSPR cytosensing afforded ultrasensitivity for cancer cell detection with the limit of detection (LOD) of 30 cells/mL and good linearity in a wide range of 1×102-1×106 cells/mL under the optimal conditions of modified AuNPs size and Con A concentration. The U-shaped fiber optic LSPR cytosensing shows more than 29 times lower limit of detection than the straight ones. More importantly, the prepared U-shaped cytosensing was applied to evaluate the N-glycan expression level on the surface of cancer cells under different concentration external stimuli of inhibitor tunicamycin (TM). Noticeably, U-shaped fiber optic LSPR cytosensing showed an acceptable reproducibility, satisfactory anti-interference and good selectivity, and was also applied in the assessment of N-glycan expression on surface of six kinds of cell lines. Therefore, the U-shaped fiber optic LSPR offers a feasible tool in biophysical research and clinical diagnosis for cancer.
关键词： Cancer cell,N-Glycan expression,Cytosensing,U-shaped fiber optic probe,Local surface plasmon resonance
更新于2025-09-23 15:23:52
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Investigation of a low cost tapered plastic fiber optic biosensor based on manipulation of colloidal gold nanoparticles
摘要： We demonstrate a low cost Tapered Fiber Optic Biosensor (TFOBS) based on manipulation of colloidal gold nanoparticle in the evanescent field of tapered fiber. The sensor consists of a biconical tapered plastic optical fiber with colloidal gold nanoparticles coated on the tapered region. The strength of evanescent field absorption as a function of gold nanoparticle size is evaluated using Finite Difference Time Domain simulation. Simulation results suggest that in order to achieve maximum absorption in visible range of wavelength, the size of the gold nanoparticle should be in the range 3-4 nm. Gold nanoparticle of desired size is then synthesized and coated on the tapered region to fabricate a TFOBS. It is found experimentally that coating of gold nanoparticles enhances the fraction of light coming out to the cladding surface. This is explained as a consequence of local surface plasmonic resonance. The TFOBS is then tested for its sensing application to detect Bovine Serum Albumin. Level of detection of the proposed sensor is calculated and is found as 0.326gm/dL.As normal albumin level in healthy human blood serum is between 3.5 to 5.5gm/dL, the proposed TFOBS can be used as a low cost accurate albumin sensor.
关键词： Tapered Fiber Optic Sensor,Local Surface Plasmon Resonance,Manipulation of nanoparticle
更新于2025-09-23 15:22:29
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Plasmonic enhanced photoelectrochemical aptasensor with D-A F8BT/g-C3N4 heterojunction and AuNPs on a 3D-printed device
摘要： D-A F8BT/g-C3N4 type II heterojunction nanocomposite was employed as photoelectrode material for photoelectrochemical (PEC) aptasensor based on rolling circle amplification (RCA) with the assistance of 3D-printed device for carcinoembryonic antigen (CEA) detection. D-A type F8BT improved photocurrent response of g-C3N4 nanosheet after forming D-A F8BT/g-C3N4 type II heterojunction which effectively constructed built-in electric field and reduced electron-hole recombination. With the introduction of DNA-AuNP probes by π-stacking interaction, the local plasmon resonance (LSPR) of AuNPs enhanced local electric field, promoted the generation of electron-hole and further improved photocurrent response. When target CEA was in presence of sensing platform, sandwich construction was formed between two kinds of CEA aptamers and CEA on magnetic beads, triggering the RCA reaction to produce repeated sequences. Partial sequences of complementary-DNA in combination with RCA results was digested by Exo III enzyme to release signal-DNA, detaching DNA-AuNP probes from the surface of the F8BT/g-C3N4 by complementation, causing the attenuation of LSPR and the decrease of photocurrent response. Combing with 3D-printed device, this PEC aptasensor exhibited a linear response to CEA from 0.02 ng mL?1 to 50 ng mL?1 with detection limit of 6.7 pg mL?1, contributing to develop all organic polymer semiconductors as photoelectric materials in PEC study.
关键词： Type II heterojunction,Rolling circle amplification,Photoelectrochemical aptasensor,D-A F8BT/g-C3N4 nanocomposites,3D-printed device,Local surface plasmon resonance
更新于2025-09-23 15:19:57
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Tunable local surface plasmon resonance and refractive index sensitivity ability of three-layered dielectric-silver bimetallic nanoshells
摘要： Optical properties and refractive index sensitivity abilities of three-layered silver/gold-dielectric-silver bimetallic nanoshells are investigated based on quasi-static theory. The results show that absorption spectra of silver/gold-dielectric-silver bimetallic nanoshells can be well tuned by changing the inner core radius, middle dielectric radius and outer shell layer thickness. Local surface plasmon resonance (LSPR) shifts of |ω? ?? mode show a red-shift with increasing the inner core radius and the thickness of middle dielectric layer, while a blue-shift with increasing the thickness of outer shell. It is interesting to find that the shifts of |ω+ ?? mode are related to the ratio ε2∕ε4, the component of core and nanoshell. In addition, the refractive index sensitivity abilities of three-layered silver/gold-dielectric-silver bimetallic nanoshells are also reported. A higher refractive index sensitivity ability can be obtained by adjusting its geometric size and component. |ω? ?? mode gets more intensity with increasing the surrounding medium dielectric function, while |ω+ ?? mode fades down. The intensity difference between |ω? ?? mode and |ω+ ?? mode is mainly effected by the surrounding medium dielectric function, its geometric parameter, middle layer dielectric function, the component of core and nanoshell. Our study provides a way to analyze sensitivity application of nanoshells and broadens the application of nanoshells in sensor ability.
关键词： Plasmon hybridization theory,Local surface plasmon resonance (LSPR),Dielectric-silver bimetallic nanoshells,Refractive index sensitivity ability
更新于2025-09-19 17:15:36
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Photoluminescence Enhancement of Perovskite CsPbBr3 Quantum Dots by Plasmonic Au Nanorods
摘要： Plasmonic Au nanorods/CsPbBr3 quantum dots film has been prepared via spin-coating. It is found that photoluminescence intensity of Au nanorods/CsPbBr3 quantum dots film exhibits 2-fold enhancement compared with pristine CsPbBr3 quantum dots film. Time-resolved photoluminescence decay curves show that the lifetime of CsPbBr3 quantum dots film decreases from 6.15 ns to 4.78 ns after combining with Au nanorods. The finite-difference time-domain simulation indicates that Au nanorods arouse a localized surface plasmon resonance around 500 nm, which enhances the emission intensity of CsPbBr3 quantum dots film.
关键词： CsPbBr3 Quantum Dot,Photoluminescence,Local Surface Plasmon Resonance,Au Nanorods
更新于2025-09-12 10:27:22