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Integration of fluorescence imaging and electrochemical biosensing for both qualitative location and quantitative detection of cancer cells
摘要： In this work, DNA-templated silver nanoclusters (DNA-AgNCs) with unique fluorescent and electrochemical properties are prepared as dual signal probes for both qualitative imaging and quantitative detection of cancer cells in an integrated system. ITO electrode that has good light transmittance and electric conductivity is employed as a substrate for dual analysis of cancer cells. ITO electrode is firstly modified by AS1141 aptamer, which could selectively bind to nucleolin overexpressed on the surface of a model breast cancer cell, MCF-7 cell line. The composite of mucin 1 antibody (anti-MUC1) and DNA-AgNCs then binds to MUC1 on the surface of captured MCF-7 cell, forming a sandwich-like structure. Therefore, our method allows noninvasive fluorescence imaging and amplified electrochemical detection using a single labeling platform, providing a biocompatible and highly specific method for adequate analysis of cancer cells. Experimental results demonstrate that strong red fluorescence of DNA-AgNCs clearly displays the loading of cancer cells on ITO electrode after dual recognition, and amplified electrochemical signals of DNA-AgNCs enable improved sensitivity toward quantitative analysis with a detection limit of 3 cells.
关键词： ITO electrode,DNA-templated silver nanoclusters,MCF-7 cell,fluorescent imaging,electrochemical detection
更新于2025-09-23 15:22:29
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Synthesis of folic acid conjugated photoluminescent carbon quantum dots with ultrahigh quantum yield for targeted cancer cell fluorescence imaging
摘要： Folic acid functionalized carbon quantum dot (FA-CQD) with ultrahigh quantum yield (50%) were synthesized by one-pot hydrothermal route using citric acid. The synthesized CQDs were characterized by fluorescence spectroscopy, transmission electron microscopy (TEM), dynamic light scattering (DLS) and X-ray diffraction. The cell viability of about 95% and 97% were obtained for MTT assay of the CQDs and FA-CQDs toward MCF-7 cells after 24 h of incubation respectively. The FA-CQDs were successfully applied for targeted imaging of cervical cancer (type HeLa) and human breast adenocarcinoma (type MCF7) cells using fluorescence microscope.
关键词： HeLa cancer cell,Ultra high quantum yield CQDs,MCF-7 cancer cell,Fluorescence imaging,Targeted cancer imaging
更新于2025-09-23 15:19:57
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Secondary toxic effect of graphene oxide and graphene quantum dots alters the expression of miR-21 and miR-29a in human cell lines
摘要： For in vitro studies, non-toxic doses of nanomaterials are routinely selected by quantification of live cells after exposing to different concentrations of nanoparticles but considering only morphological changes or viability of cells is not sufficient to conclude that these nanomaterials are non-cytotoxic. Here we investigated if secondary toxicity is active in the cells exposed to non-toxic doses of graphene oxide (GO) and graphene quantum dots (GQD). Non-cytotoxic dose of 15 μg mL-1 of GO (100 nm) and GQDs (50 nm) was selected according to MTT and Hoechst 33342/PI double staining assays. In order to investigate the secondary toxicity, the expression of miR-21, miR-29a and three genes at both mRNA and protein level were evaluated in MCF-7, HUVEC, KMBC/71 cells 4 and 24 h post exposure. Mitochondrial membrane potential (MMP) was assessed by Rhodamine 123 staining. According to our results, there was no significant decrease in viability of cells after exposure to the no-cytotoxic dose of GO and GQD, but we observed significant alterations in the expression level of miR-21, miR-29a, Bax, Bcl2 and PTEN genes after treatment in all three cells. In addition to molecular changes, we observed alteration in mitochondrial activity at cellular level. However, we also observed that GO influenced the basal level of genes and influenced MMP more compare to GQDs-50. Considering that all these genes are involved in breast tumor development and metastasis, the observed changes in miRNA expression and protein synthesis may alter cell fate and susceptibility and cause deviation in the desired outcome of GO-100 and GQDs-50 application in medical researches.
关键词： Graphene oxide,MCF-7 cells,HUVEC cells,MicroRNA-21,Graphene quantum dots,MicroRNA-29a
更新于2025-09-23 15:19:57
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An improved class of fluorescent silica nanoparticles for indirect immunofluorescence detection of MCF-7?cells
摘要： In this work, we have developed an efficient method based on silica fluorescent dye-doped nanoparticles and second antibody for cancer cell labeling. The dye-doped silica nanoparticles were synthesized by the reverse microemulsion method with the conjugate of 3-aminopropyltriethoxysilane and rhodamine B isothiocynate. A PEG with flexible long chain as the bridge was introduced onto the surface of the nanoparticle via cyanogens bromide method. The second antibody, goat anti-rabbit IgG, was conjugated on the surface of the PEG-terminal modified silica fluorescent nanoparticles by covalent binding to the PEG linkers via the EDC/sulfo-NHS method. The concentrations of goat anti-rabbit IgG covering the nanoparticles were quantified via the Bradford method. The silica fluorescent nanoparticles functionalized with rabbit anti-MUC1 antibody were employed as bioprobes for the recognition of MUC1 protein in human breast carcinoma molecules MCF-7 cells. Compared with fluorescent dye labeled IgG, the fluorescent nanoprobes display dramatically increased photostability.
关键词： MCF-7 cells,Dye-doped silica nanoparticles,Conjugation,Mucin,Bioprobe
更新于2025-09-23 15:19:57
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Determination of Infrared Laser Energy Dose for Cancer Cells Inactivation as a Candidate of Photodynamic Therapy
摘要： The aim of this research is to find out the effect of GaAlAs diode laser 808 nm exposure on MCF-7 breast cancer cells in vitro with and without the addition of a photosensitizer. Methylene blue (C16H18N3SCl) with concentration of 2 μM is used as the photosensitizer based on the preliminary results of cytotoxicity assay. Energy dose is set in the range of 23,043 to 322,062 J/cm2. Laser exposure with the addition of the photosensitizer generates the highest percentage of cell death of 20.80% at energy dose of 184,344 J/cm2 and cell death begin to decrease at energy dose above this value. This behavior is likely caused by photodegradation and photobleaching effect of the photosensitizer due to the longer exposure time. However, the percentage of cell death without the addition of photosensitizer is increased proportionally to the increase of energy dose and achieved 32,45% cell mortality at 299,559 J/cm2. This study not only shows that the exposure of infrared laser can be used to inactivate cancer cells but also determines its optimum energy dose, makes it a possible candidate for photodynamic therapy in the future.
关键词： MCF-7 cells,photodynamic therapy,photochemical,methylene blue,diode laser,photothermal
更新于2025-09-16 10:30:52
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Glutathione Capped Core/shell CdSeS/ZnS Quantum Dots as a Medical Imaging tool for Cancer Cells
摘要： Nanomaterials have secured an enduring position as a promising contender for various biological applications. Quantum dot (QD) is one such example of nano sized semiconductor material having unique optical characteristics that makes it an ideal candidate to be used as imaging tool for today’s medical industry. In the present study, the L-Glutathione (L-GSH) capped L-GSH-CdSeS/ZnS core/shell QDs were applied to visualize MCF-7 human breast cancer cells. Time and dose dependent studies were done, to study the cell penetration and visualization of QDs within the cancer cells. MTT assay was done to derive the percent cell viability of cancer cells at different QDs dose concentrations. Cell cytotoxicity of the capped CdSeS/ZnS QDs were also studied on non-cancerous healthy cells. Human cancerous cells were treated with the selected doses of QDs for different time durations followed by counter staining of the cells with fluorescent DAPI nuclear stain to observe the reflected QD mediated effects. The small sized QDs were localized predominantly in the cytoplasm of MCF-7 cells and displayed no significant cytotoxicity on healthy cells. Our findings suggest that our new synthesized L-GSH-CdSeS/ZnS core/shell QDs could be highly biocompatible and can have a high probability of applications in bio-labeling and imaging for cancer diagnostics.
关键词： Diagnostics,CdSeS/ZnS Quantum dot,Toxicity,Biocompatible,Cancer,Cell death,MCF-7
更新于2025-09-12 10:27:22
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Photophysical insights and in vitro cytotoxicity of dyes-gold nanostars system towards MCF-7 and A-549 cancer cells
摘要： In this paper, the interaction of phenothiazine (PHZ) dyes (Azure A (AZA) and thionine (TH)) with gold nanostars (Au NSs) were studied using absorption spectroscopy, steady state emission spectroscopy, high resolution transmission electron microscopy (HR-TEM), dynamic light scattering (DLS), Zetapotential measurements and Fourier transform infrared (FT-IR) spectroscopy. Absorption spectral studies of Au NSs in the presence of dyes resulted in a slight red shift on the surface plasmon resonance band (SPR) of Au NSs, suggesting the changes in the Au NSs surface owing to the adsorption of dyes. The phenomena of hypochromism and the appearance of coupled localized surface plasmon resonance (LSPR) bands in the absorption spectral studies of the AZA-Au NSs and TH–Au NSs systems suggested the vital role of electrostatic interactions between PHZ dyes and Au NSs. The formation of the ground state complex between dyes and Au NSs were evident from the outcome of the steady-state emission titration experiments of the dyes-Au NSs system. Further, the cytotoxic activity of AZA, TH, AZA-Au NSs and TH-Au NSs complexes on breast cancer (MCF-7) and A-549 cell lines were also investigated. These studies revealed that AZA-Au NSs, TH-Au NSs complexes showed the higher level of cytotoxicity than PHZ dyes treated cells.
关键词： A-549 and MCF-7 cells,Goldnanostars,Thionine,DLS,Azure A,HR TEM
更新于2025-09-09 09:28:46
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Photodynamic Therapy Based on Arrabidaea chica (Crajiru) Extract Nanoemulsion: In vitro Activity against Monolayers and Spheroids of Human Mammary Adenocarcinoma MCF-7 Cells
摘要： Natural products have been sources of numerous drugs over the history. However, little is known about the therapeutic potential of Amazon forest species. This work aimed at testing the potential of the hydrophobic extract of Arrabidaea chica (ACE), an Amazon plant, as a source of photosensitizers for anticancer photodynamic therapy. ACE was tested as a nanoemulsion (ACE-NE) produced by phase inversion temperature. It was found that ACE-NE intensely absorbs red light and, under photoactivation (λ 660 nm, 4.5 J/cm2), produces reactive oxygen species. Photoactivated ACE-NE presented, in vitro, a CC50 of 1.3 μg ACE/mL against human breast adenocarcinoma MCF-7 cells, and was effective in lysing MCF-7 spheroids. In the dark, ACE was toxic neither to human mammary epithelial MCF-10A cells nor to MCF-7 cells. On that ground, this work is the first to show that A. chica is a source of photosensitizers potentially useful for anticancer photodynamic therapy.
关键词： Amazon natural extracts,Cancer,Spontaneous emulsification,Human breast adenocarcinoma MCF-7 cells,Spheroids
更新于2025-09-09 09:28:46