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Blue Copper Peroksidase and Phthalocyanine Conjugate: Synthesis, Characterization and Applications
摘要： Trametes versicolor can degrade barks as a source for carbon necessity. Therefore it secretes lignin peroxidase, mangan peroxidase and laccase. The laccase enzyme was produced high yield at pH and glucose concentration 5 and 10 gL-1, respectively. In optimized medium, the enzyme activity was between 200-250 UL-1 when inducer was absent. It was seen that the activity reached 400 UL-1 when phenol used as an inducer. The molecular weight of purified laccase was found 80 kDa with SDS-PAGE and kinetic constant Km and Vmax values for ABTS were determined 3.66x10-4 μM and 1652 UL-1, respectively. Hence, due to these properties, these enzymes are widely used in industrial areas free or immolized. Laccase enzyme decolorization of 6 different dyes was carried out. A decolorization capacity of 50-99% was achieved by cultivation in 20 days using a beginning dye concentration of 20 ppm. The removal of color with active enzyme is obtained around 90%. Also laccase enzyme was conjugated amine functionalized low symmetrically phthalocyanine. This conjugate was examined both photodynamic therapy and chemosensor application. This conjugate fluorescence had a quantum yield of 0.32 (lifetime 3.59 ns) and generates efficiently singlet oxygen (quantum yield 0.4). The conjugate was successfully photodamage displayed in HeLa and HuH-7 cells in photodynamic therapy application. These results indicate that conjugate represent interesting agent with potential applications in photodynamic therapy. In addition that the chemosensor behavior of this compound to different metal ions has been studied and this conjugate display as fluorescence chemosensor for determination of Fe3+ions.
关键词： Conjugate,Zinc phthalocyanine,Metal Sensor,Laccase,Photodynamic Therapy
更新于2025-09-23 15:23:52
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Selectivity of chemosensor phthalocyanines for Pd2+ ions; synthesis, characterization, quantum chemical calculation, photochemical and photophysical properties
摘要： In this study, ligand (1) was prepared by a nucleophilic displacement reaction of (4-bromophenyl)methanethiol with 4-nitrophthalonitrile according to literature and its novel type peripheral substituted tetra phthalocyanines [CuPc (2), ZnPc (3) and GaPc (4)] were prepared. The phthalocyanines obtained from compund (1) were characterized by FT-IR, 1H-NMR, UV-Vis spectral data, elemental analysis, and MALDI-TOF. The novel phthalocyanines [CuPc (2), ZnPc (3) and GaPc (4)] were highly soluble in common organic solvents such as CHCl3, THF, CH2Cl2, DMSO and DMF. The effect of Pd2+ ions on the fluorescence emission and UV-Vis spectra were performed with addition of increasing amounts of Pd2+ ions. This functional phthalocyanines exhibited H-type aggregation versus Pd2+ ions due to special chemical structures, which can be used as a selective chemosensor for Pd2+ ions. Also, the molecular reactivity of the ligand (1) and related metallo phthalocyanines [CuPc (2), ZnPc (3) and GaPc (4)] were investigated and compared with the analysis of frontier molecular orbitals. In addition, photochemical and photophysical properties of these new phthalocyanines (fluorescence quantum yields, fluorescence behavior, singlet oxygen and photodegradation quantum yields) were studied in DMSO.
关键词： Phthalocyanine,Photochemical,Photophysical,Quantum Chemical Calculation,Metal sensor
更新于2025-09-23 15:21:21