- 标题
- 摘要
- 关键词
- 实验方案
- 产品
-
Monitoring unbound warfarin in drug combination therapy by pharmacokinetics and fluorospectrometry
摘要： Objective: Monitoring the unbound drug concentration in blood in combination therapy is necessary, because its concentration determines the efficacy of drug therapy. This study was designed to explore the effect of Dan Hong Injection (DHI) on the unbound warfarin using two approaches including an in vivo pharmacokinetic and in vitro fluorescence studies. Methods: The effect of DHI on the pharmacokinetic properties of the unbound warfarin was investigated by a microdialysis sampling method coupled with LC–MS/MS. The effect of DHI and salvianolic acid B (SaB) on warfarin binding with bovine serum albumin (BSA) was conducted by fluorescence spectrometry. Results: The AUC 0-tn of warfarin with DHI group was higher than that of warfarin alone group. The result showed that DHI could increase the concentration of unbound warfarin in rat blood, which may be due to the competition between warfarin and DHI as well as its components binding to serum albumin. The competition process was demonstrated by fluorescence study. Conclusion: Combination therapy of DHI with warfarin could enhance the release profile of warfarin from serum protein.
关键词： microdialysis,unbound warfarin,pharmacokinetics,fluorospectrometry,Dan Hong Injection
更新于2025-09-23 15:23:52
-
Co Incorporated Mesoporous Carbon Material Assisted Laser Desorption/Ionization Ion Source as an on-line Interface of in vivo Microdialysis Coupled with Mass Spectrometry
摘要： The combination of microdialysis and mass spectrometry (MS) provides the potential for rapidly monitoring diverse metabolites in vivo. Unfortunately, the high concentration of salt in biological microdialysates hindered the sensitive and on-line detection of these small molecular compounds. In this study, we synthesized Co incorporated mesoporous carbon material (Co-NC), and developed Co-NC assisted laser desorption/ionization (LDI) ion source as an on-line interface of in vivo microdialysis coupled with MS for direct analysis of diverse metabolites in microdialysates. The Co-NC could be used as matrix for surface assisted laser desorption/ionization mass spectrometry (SALDI MS) analysis of small molecular compounds, even under high concentration of salt conditions. The Co-NC possessed the adsorption ability for small molecular compounds, and it was believed that the adsorption ability of Co-NC might separate the analytes from the salt in microdialysates at a microscopic level, which might facilitate the desorption and ionization of the analytes and finally improved the salt-tolerance ability as matrix. Furthermore, the Co-NC assisted LDI ion source as a novel interface of in vivo microdialysis coupled with MS has been applied to the on-line monitoring of liver metabolites from the CCl4-induced liver injury rat model for the first time.
关键词： microdialysis,Co incorporated mesoporous carbon material,metabolites,mass spectrometry,laser desorption/ionization,salt-tolerance
更新于2025-09-23 15:19:57
-
[IEEE 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems & Eurosensors XXXIII (TRANSDUCERS & EUROSENSORS XXXIII) - Berlin, Germany (2019.6.23-2019.6.27)] 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems & Eurosensors XXXIII (TRANSDUCERS & EUROSENSORS XXXIII) - Picoliter Droplet Generation for Fast Monitoring the Brain Chemistry with Scaled Silicon Nanodyalisis Probe
摘要： To monitor neurochemicals while minimizing brain damage, a microdialysis system is developed with fluidic channels scaled to 5 μm-radius to fit into 15x50 μm2 silicon neural probe. Droplet generation is utilized to halt Taylor dispersion to achieve high temporal resolution. To extend the stability region for monodisperse droplet generation in such a space-limited probe at ultra-low nL/min flow rates, we varied the T-junction angle, parameter that is typically omitted from consideration for larger channels. In a series of experiments, we found that increase of the T-junction angle increases the critical capillary number separating squeezing and regimes. With jetting segmentation optimized geometry, we demonstrated generation of monodisperse pL-volume droplets in silicon nanofluidic channels. Finite element analysis indicated that these effects are due to interplay between differential pressure and viscous shear forces.
关键词： Neurochemistry,MEMS,Droplet generation,Microdialysis,Implantable neural probe
更新于2025-09-11 14:15:04