- 标题
- 摘要
- 关键词
- 实验方案
- 产品
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?Lab on a Chip“; Lab on a Chip;
摘要： Background. Miniaturization has not only driven microelectronics and generated new unforeseen options but has also dramatically changed sensors and analytics. Developments. The Lab on a Chip (LOC) technology enables laboratory processes to run fully automated in canals in the micrometre range. The biggest challenge for LOC is to keep production costs low despite miniaturization and application-specific design. If this is achieved medical laboratory analyses can usually be carried out faster and with less hands on time. This explains why LOCs are already integrated into many laboratory instruments and why point-of-care testing (POCT) can no longer be imagined without it. New markers, such as in liquid biopsies and measurement techniques, such as Raman spectroscopy and mass spectroscopy, create further potentials that will enable faster and more specific laboratory analyses to be made using LOC technology. Conclusion. The LOC technology has the potential of changing the medical practice especially in cases when the central laboratory is not available or is unable to provide results fast enough.
关键词： Laboratory diagnostics,Mass spectrometry,Point-of-care testing,Spectrum analysis, Raman,Microfluidics
更新于2025-09-10 09:29:36
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Design of Controllable Novel Piezoelectric Components for Microfluidic Applications
摘要： This paper reviews recent investigations and achievements in the design of controllable functional components for improving microfluidic systems, its effectiveness, and functionality. The main purpose was to design novel microstructures with piezoelectric properties (microresonators), which enable one to control the effectiveness of fluid flow in micro-hydro-mechanical devices for biomedical/biochemical purposes. Controllable properties were obtained by incorporating different types of binders in a piezoelectric ceramic matrix (lead zirconate titanate): polyvinyl butyral (PVB), poly methyl methacrylate (PMMA), and polystyrene (PS). The change in chemical composition of PZT helps to manipulate the piezoelectric characteristics, surface morphology, mechanical properties, etc., of the designed microfluidic element with the microstructure in it.
关键词： microfluidics,chemical composition,piezoelectric,periodical microstructure,microresonator
更新于2025-09-10 09:29:36
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A smartphone-assisted microfluidic chemistry analyzer using image-based colorimetric assays for multi-index monitoring of diabetes and hyperlipidemia
摘要： A smartphone-assisted microfluidic chemistry analyzer using an image-based colorimetric detection method was successfully developed for the simultaneous analysis of three diabetes- and hyperlipidemia-related indexes, glucose (GLU), triglyceride (TG), and total cholesterol (TC). A fan-shaped microfluidic chip was designed and optimized to reliably allocate a premixed serum sample into four reaction chambers with a simple pipetting. The color changes of the peroxidase-H2O2 enzymatic reactions in the chambers were captured and analyzed using a smartphone-controlled analyzer with a LED light source and a CCD camera. The highly quantitative relationships between the analyte concentrations and the color characteristic values of the green channel of the captured images were successfully established, enabling accurate and reproducible detections of GLU, TG, and TC simultaneously at a low cost. The parallel analyses of 111 serum samples using our system and the conventional chemistry analyzer were conducted, yielding an excellent correlation and consistency between these two methods. This study proved the feasibility of performing the multi-index monitoring of diabetes, hyperlipidemia, and other chronic diseases on a POCT platform at a high fidelity, but a low cost.
关键词： Point-of-care testing,Smartphone,Colorimetric detection,Microfluidics,Multi-index analysis
更新于2025-09-10 09:29:36
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From chip-in-a-lab to lab-on-a-chip: a portable Coulter counter using a modular platform
摘要： The field of microfluidics has been struggling to obtain widespread market penetration. In order to overcome this struggle, a standardized and modular platform is introduced and applied. By providing easy-to-fabricate modular building blocks which are compatible with mass manufacturing, we decrease the gap from lab-to-fab. These standardized blocks are used in combination with an application-specific fluidic circuit board. On this board, electrical and fluidic connections are demonstrated by implementing an alternating current Coulter counter. This multipurpose building block is reusable in many applications. In this study, it identifies and counts 6 and 11 μm beads. The system is kept in a credit card-sized footprint, as a result of in-house-developed electronics and standardized building blocks. We believe that this easy-to-fabricate, credit card-sized, modular, and standardized prototype brings us closer to clinical and veterinary applications, because it provides an essential stepping stone to fully integrated point-of-care devices.
关键词： modular platform,point-of-care devices,lab-on-a-chip,microfluidics,Coulter counter
更新于2025-09-10 09:29:36
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Ultrasensitive Refractometry <i>via</i> Supercritical Angle Fluorescence
摘要： Refractometry, namely, the measurement of refractive index (RI), provides information about various sample properties such as concentrations and molecular structure. One physical phenomenon which enables precise determination of a sample’s RI in a microscope is the supercritical-angle fluorescence. This effect is observed when the fluorescence from an emitter near a glass-medium interface is captured by an objective lens with a high numerical aperture. The materials’ index mismatch creates a distinguishable transition in the intensity pattern at the back focal plane of the objective that changes proportionally to the RI of the media. Here, we present a refractometry approach in which the fluorophores are preattached to the bottom surface of a microfluidic channel, enabling highly-sensitive determination of the RI using tiny amounts of liquid (picoliters). With this method, we attained a standard deviation of 3.1 × 10?5 and a repeatability of 2.7 × 10?5 RI units. We first determine the capabilities of the device for glycerol-water solutions, then demonstrate the relevance of our system for monitoring changes in biological systems. As a model system, we show that we can detect single bacteria (Escherichia coli) and measure population growth.
关键词： refractive-index,refractometry,bacterial growth,microfluidics,supercritical angle fluorescence,bio-detection
更新于2025-09-09 09:28:46
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Numerical Demonstration of In-Tube Liquid-Column Migration Driven by Photoisomerization
摘要： Droplet manipulation by light-induced isomerization was numerically demonstrated and investigated regarding the driving mechanism. Such a non-invasive manipulation of a droplet in a microchannel can be realized, for example, by the use of watery solution of photoresponsive surfactant that exhibits the isomerization. Due to variable fluid properties between the cis and trans isomers, one-side light irradiation on a liquid column in a tube would lead to some kind of imbalance between the two ends of the liquid column and then drive droplet migration. The present numerical simulations of air–liquid two-phase flow and its scalar transport of the isomer, considering the variable static contact angle, agreed quantitatively with the experimental results in terms of the migration speed. This fact supports the contention that the droplet migration is more likely to be driven by an imbalance in the wettability, or the contact angle. The migration speed was found to be less dependent on the liquid-column length, but proportional to the tube diameter.
关键词： photochemical reaction,non-invasive control,wettability,surface tension,microfluidics,computational fluid dynamics,lab-on-a-chip,droplet manipulation,two-phase flow,photoresponsible surfactant
更新于2025-09-09 09:28:46
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Direct Stereolithographic 3D Printing of Microfluidic Structures on Polymer Substrates for Printed Electronics
摘要： Both stereolithographic printing of microfluidics and inkjet printing of electronics are promising tools for the fabrication of lab-on-a-chip devices. However, the combination of these two technologies has been a challenge so far, as the 3D-printed components usually have to be bonded manually to the substrates functionalized with printed electronics. Here, a surface modification method is demonstrated for enabling the direct stereolithographic printing of microfluidic structures onto a variety of different substrates that are usually employed for printed electronics. The approach makes use of an acrylate-terminated silane that covalently binds substrate and polymer network of the 3D print. The bonding strength is quantified and the compatibility of the concept with printed electrodes in a microfluidic channel is evaluated.
关键词： additive manufacturing,3D printing,printed electronics,microfluidics
更新于2025-09-09 09:28:46
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Microchannel Fabrication Using A Photo-Patternable Adhesive Material for Recording Conformational Changes of KcsA Channel with the Diffracted X-ray Tracking Method
摘要： Diffracted X-ray tracking (DXT) method can trace conformational changes of KcsA potassium ion channel during gating by recording position of diffraction spot from a gold nanocrystal attached to the channel as a movie. For high-resolution imaging under controlled microenvironments for KcsA channels, we report a microfluidic device consisting of two SiN membrane windows bonded with a photo patternable adhesive material. The reduced signal-to-background ratio as well as suitable adhesive material thickness for the microchannel are discussed in the experiment at the synchrotron radiation facility.
关键词： diffracted X-ray tracking method,adhesive bonding,microfluidics,KcsA ion channel
更新于2025-09-04 15:30:14
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Dynamic Microfluidic Cytometry for Single-Cell Cellomics: High-throughput Probing Single-Cell-Resolution Signaling
摘要： Cell signaling is a fast dynamic and complex process, which controls a variety of critical physiological functions. Methods to investigate such dynamic information, however, suffer from limited throughput in single cells level and a lack of precise fluid manipulation. Herein, we present a new strategy, termed dynamic microfluidic cytometry (DMC), for high throughput probing GPCR signaling in single cells resolution (single-cell cellomics analysis) by creatively applied cyclical cell trapping, stimulating and releasing automatically. Dose-response curves and EC50 values for HeLa cells treated with adenosine triphosphate (ATP), histamine (HA) and acetylcholine chloride (ACH) were successfully obtained in single-cells level. High throughput single-cells dynamic signaling were further implemented by sequential or simultaneous stimulation, which revealed that different mechanisms were working in triggering intracellular calcium releasing. In addition, simultaneous stimulating to two different types of cells, HeLa and NIH-3T3 cells, was also successfully realized, which was crucial for online comparison dynamic signaling of different types of cells. We believe that the proposed DMC provides a versatile means for high throughput probing single cell dynamic signaling, which is potentially useful in chemical biology, cell biology and pharmacology.
关键词： dynamic signaling,cytometry,microfluidics,single-cell cellomics
更新于2025-09-04 15:30:14
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Sorting and manipulation of biological cells and the prospects for using optical forces
摘要： Contemporary biomedical research requires development of novel techniques for sorting and manipulation of cells within the framework of a microfluidic chip. The desired functions of a microfluidic chip are achieved by combining and integrating passive methods that utilize the channel geometry and structure, as well as active methods that include magnetic, electrical, acoustic and optical forces. Application of magnetic, electric and acoustics-based methods for sorting and manipulation have been and are under continuous scrutiny. Optics-based methods, in contrast, have not been explored to the same extent as other methods, since they attracted insufficient attention. This is due to the complicated, expensive and bulky setup required for carrying out such studies. However, advances in optical beam shaping and computer hardware, and software have opened up new opportunities for application of light to development of advanced sorting and manipulation techniques. This review outlines contemporary techniques for cell sorting and manipulation, and provides an in-depth view into the existing and prospective uses of light for cell sorting and manipulation.
关键词： Cell manipulation,Optical sorting,Microfluidics,Acoustic sorting,Cell separation,Dielectrophoresis,Magnetic manipulation
更新于2025-09-04 15:30:14