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A multifunctional platform with single-NIR-laser-triggered photothermal and NO release for synergistic therapy against multidrug-resistant Gram-negative bacteria and their biofilms
摘要： Background: Infectious diseases caused by multidrug-resistant (MDR) bacteria, especially MDR Gram-negative strains, have become a global public health challenge. Multifunctional nanomaterials for controlling MDR bacterial infections via eradication of planktonic bacteria and their biofilms are of great interest. Results: In this study, we developed a multifunctional platform (TG-NO-B) with single NIR laser-triggered PTT and NO release for synergistic therapy against MDR Gram-negative bacteria and their biofilms. When located at the infected sites, TG-NO-B was able to selectively bind to the surfaces of Gram-negative bacterial cells and their biofilm matrix through covalent coupling between the BA groups of TG-NO-B and the bacterial LPS units, which could greatly improve the antibacterial efficiency, and reduce side damages to ambient normal tissues. Upon single NIR laser irradiation, TG-NO-B could generate hyperthermia and simultaneously release NO, which would synergistically disrupt bacterial cell membrane, further cause leakage and damage of intracellular components, and finally induce bacteria death. On one hand, the combination of NO and PTT could largely improve the antibacterial efficiency. On the other hand, the bacterial cell membrane damage could improve the permeability and sensitivity to heat, decrease the photothermal temperature and avoid damages caused by high temperature. Moreover, TG-NO-B could be effectively utilized for synergistic therapy against the in vivo infections of MDR Gram-negative bacteria and their biofilms and accelerate wound healing as well as exhibit excellent biocompatibility both in vitro and in vivo. Conclusions: Our study demonstrates that TG-NO-B can be considered as a promising alternative for treating infections caused by MDR Gram-negative bacteria and their biofilms.
关键词： Biofilms,Single-NIR-laser-triggered,Graphene,NO release,Multidrug-resistant Gram-negative bacteria,Synergistic,Photothermal
更新于2025-09-23 15:19:57
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Supermagnetic Fe <sub/>3</sub> O <sub/>4</sub> -PEG nanoparticles combined with NIR laser and alternating magnetic field as potent anti-cancer agent against human ovarian cancer cells
摘要： In the present study the magnetite nanoparticles Fe3O4 capped with polyethylene glycol PEG were prepared by hydrothermal method. Syntheses bare Fe3O4, and Fe3O4-PEG were confirmed by various techniques including X-ray powder diffraction (XRD), Transmission electron microscope (TEM), Dynamic light scattering (DLS), Scanning electron microscope (SEM), and Vibrating sample magnetometer (VSM) techniques were used to examine the magnetic properties of the Fe3O4 MNPs. The bare Fe3O4 had an average size that ranged between 12-31nm while Fe3O4- PEG had a size range of 5-15 nm. The effect of bare Fe3O4 and Fe3O4-PEG on SKOV-3 cell proliferation was measured by MTT assay. The ability of bare Fe3O4 and Fe3O4-PEG in induction of apoptosis was tested using Acridine orange/ Ethedium bromide stain. Cytotoxicity effects of bare Fe3O4 and Fe3O4-PEG against SKOV-3 cells in the presence and absence of laser photo-thermal therapy and alternating magnetic field (AMF) was also assessed. The results showed that both bare Fe3O4 and Fe3O4-PEG caused inhibition of SKOV-3 cell proliferation, resulting in their programmed death. Cytotoxic activity against SKOV-3 cells increased with NIR laser irradiation, while highly increased cytotoxic activities were observed after exposure to induction heating with AMF. In addition, the toxicity of investigated nanoparticles in liver function enzymes and histological alterations for spleen, and the lungs were addressed. The findings indicated that these nanoparticles were biocompatible with liver function enzymes and no significant alterations were recorded in the spleen, and the lungs. Taken together the present data suggest that bare Fe3O4 and Fe3O4-PEG with NIR laser and AMF could be promising for photothermal therapy protocol for cancer cells.
关键词： NIR laser,Hydrothermal synthesis,SKOV-3 cells,anticancer activity,Fe3O4-PEG,AMF
更新于2025-09-11 14:15:04