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Cancer cella??specific protein delivery by optoporation with lasera??irradiated gold nanorods
摘要： The delivery of macromolecules into living cells is challenging since in most cases molecules are endocytosed and remain in the endo-lysosomal pathway where they are degraded before reaching their target. Here, a method is presented to selectively improve cell membrane permeability by nanosecond laser irradiation of gold nanorods (GNRs) with visible or near-infrared irradiation in order to deliver proteins across the plasma membrane, avoiding the endo lysosomal pathway. GNRs were labelled with the anti-EGFR (epidermal growth factor receptor) antibody Erbitux to target human ovarian carcinoma cells OVCAR-3. Irradiation with nanosecond laser pulses at wavelengths of 532 nm or 730 nm is used for transient permeabilization of the cell membranes. As a result of the irradiation, the uptake of an anti-Ki-67 antibody was observed in about 50 % of the cells. The results of fluorescence lifetime imaging show that the GNR detached from the membrane after irradiation.
关键词： gold nanorods,optical window,protein delivery,near infrared radiation,nanosecond pulsed laser
更新于2025-09-23 15:19:57
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Real 3D approach vs 2D camera with and without real-time near-infrared imaging with indocyanine green for detection of endometriosis: a case-control study
摘要： Introduction: The complete surgical removal of endometriosis lesions is not always feasible because some implants may be very small or hidden. The use of intra-operative near-infrared radiation (NIR) imaging after intravenous injection of indocyanine green (ICG) coupled with robotic technical advances, including tridimensional and high-resolution vision, might improve detection rates. Material and methods: This is a retrospective, multicenter case-control study (Canadian Task Force classification II-2) on medical records of women with endometriosis that submitted to surgery at the Catholic University of Rome (controls) and the University of Bologna (Cases) between January, 2016, and March, 2018. Surgical and post-surgical data from the procedures were collected. We compared the visual detection rate of endometriotic lesions using near-infrared radiation imaging after intravenous injection of indocyanine green (NIR-ICG) in Real 3D (Cases) with the 2D Camera approach (Controls) in symptomatic women with pelvic endometriosis. Results: Twenty cases were matched as closely as possible with 27 controls. The suspected lesions identified both with the WL and the NIR-ICG approach were 116 and 70 in the Controls (2D) and Cases (3D), respectively. Among them, 16 of 116 controls (13.8%) and 12 of 70 cases (17.1%) were identified using only NIR-ICG imaging and collected as occult lesions (p=0.536). The overall NIR-ICG lesion identification showed a positive predictive value of 97.8%, negative predictive value of 82.3%, sensitivity of 82.0%, and specificity of 97.9% for the Control group, and a positive predictive value of 100%, negative predictive value of 97.1%, sensitivity of 97.1%, and specificity of 100% for the Cases group, confirming that NIR-ICG imaging is a good diagnostic and screening test (p=0.643 and p=0.791, according to the Cohen kappa tests, respectively for the laparoscopic and robotic groups). Conclusions: The few differences observed did not seem to be clinically relevant, thus making the two procedures comparable in terms of the ability to visually detect endometriotic lesions. Further prospective trials are needed to confirm our results.
关键词： near-infrared radiation,endometriosis,endometriosis surgical treatment,indocyanine green,minimally invasive surgery
更新于2025-09-19 17:13:59
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Modeling of cancer photothermal therapy using near‐infrared radiation and functionalized graphene nanosheets
摘要： Photothermal therapy using near-infrared radiation and local heating agents can induce selective tumor ablation with limited harm to the surrounding normal tissue. Graphene sheets are promising local heating agents due to their strong absorbance of near-infrared radiation. Experimental studies have been conducted to study the heating effect of graphene in photothermal therapy, yet few efforts have been devoted to the quantitative understanding of energy conversion and transport in such systems. Herein, a computational study of cancer photothermal therapy using near-infrared radiation and graphene is presented using a Monte Carlo approach. A three-dimensional model was built with a cancer cell inside a cube of healthy tissue. Functionalized graphene nanosheets were randomly distributed on the surface of the cancer cell. The effects of the concentration and morphology of the graphene nanosheets on the thermal behavior of the system were quantitatively investigated. The interfacial thermal resistance around the graphene sheets, which affects the transfer of heat in the nanoscale, was also varied to probe its effect on the temperature increase of the cancer cell and the healthy tissue. The results of this study could guide researchers to optimize photothermal therapy with graphene, while the modeling approach has the potential to be applied for investigating alternative treatment plans.
关键词： graphene nanosheet,modeling,cancer photothermal therapy,interfacial thermal resistance,near infrared radiation
更新于2025-09-11 14:15:04
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Rapid and sheet-to-sheet slot-die coating manufacture of highly efficient perovskite solar cells processed under ambient air
摘要： A nitrogen-free and slot-die coating fabrication of perovskite solar cells combined with NIR annealing. We develop a fabrication approach of planar inverted structured perovskite solar cell (PSC) in ambient condition with a PCE of 12.4% as compared to that (13.3%) of PSC fabricated in glove box filled with nitrogen. In addition, we demonstrate an alternative post-annealed method by near-infrared (NIR) radiation to traditional heating method by oven. The annealed time by NIR can be shortened from 1500 s to 30 s and the device performance over 10% can be achieved. The present work investigates the NIR effect for different layers in the two-step processing on the cell performance. The grazing-incidence wide-angle and small-angle X-ray scattering (GIWAXS and GISAXS) techniques for the perovskite layers are performed to reveal the crystalline and nano-morphological structures of perovskite layers correlated to thermal and NIR annealing effects. Based on this approach under ambient air, we scale up to the large-area fabrication using the slot-die coating. The performance of slot-die coated PSC can achieve to 12.3%. The critical step is the control of PbI2 layer thickness. We also applied the NIR radiation to the slot-die coated PSC and the PCE of 11.4% can be achieved. Our study paves a facile way to rapid manufacture and mass production of perovskite solar cells.
关键词： Perovskite solar cell,Two-steps,Near-infrared radiation,Slot-die,GIWAXS,GISAXS
更新于2025-09-09 09:28:46