- 标题
- 摘要
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- 实验方案
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Activity-based Near-Infrared Fluorogenic Probe Enables In Vitro and In Vivo Profiling of Neutrophil Elastase
摘要： Neutrophil elastase (NE), a typical hematopoietic serine protease, has significant roles in inflammatory and immune responses, and thus highly associated with various diseases such as acute lung injury (ALI) and lung cancer. Rapid and accurate measurement of NE activity in biological systems is particularly important for understanding the role of NE in inflammatory diseases, as well as the clinic diagnosis. However, the specific detection and non-invasive imaging of NE in vivo remains a challenge. To address this issue, a small-molecule substrate-based near-infrared fluorogenic probe (NEP) for NE was constructed via incorporating pentafluoroethyl as the recognition group with a hemicyanine dye-based fluorophore. This initially quenched probe possesses over than 25-fold red fluorescence enhancement upon the catalysis of human NE and the detection limit is about 29.6 ng/mL. In addition, the high specificity and the long emission wavelength (λemmax = 700 nm) of NEP allowed the direct monitoring of NE-trafficking, exogenous NE up taking and endogenous NE up-regulation at the cellular level. Moreover, the successfully spatiotemporal imaging of NE in ALI model mice also made it a promising new tool in clinical diagnosis for ALI and other lung diseases.
关键词： intracellular trafficking,Neutrophil elastase,acute lung injury.,fluorogenic probe
更新于2025-09-23 15:23:52
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Human Neutrophil Elastase Activated Fluorescent Probe for Pulmonary Diseases Based on Fluorescence Resonance Energy Transfer Using CdSe/ZnS Quantum Dots
摘要： There is an increasing demand for effective noninvasive diagnosis against common pulmonary diseases which are rising sharply due to the serious air pollution. Human neutrophil elastase (HNE), a typical protease highly involved in pulmonary inflammatory diseases and lung cancer, is a potential predictor for disease progression. Currently, few of HNE-targeting probes is applicable In Vivo due to the limitation in sensitivity and biocompatibility. Herein we reported the achievement of In Vitro detection and In Vivo imaging of HNE, by incorporating the HNE-specific peptide substrate, quantum dots (QDs) and organic dyes into the fluorescence resonance energy transfer (FRET) system. The refined nanoprobe, termed as QDP, could specifically measure the HNE with excellent sensitivity of 7.15 pM at aqueous solution, and successfully image the endogenous and exogenous HNE in living cells. In addition, this nanoprobe enabled the HNE imaging in the mouse models of lung cancer and acute lung injury, and continuously monitored the HNE activity at high temporal and spatial resolution. Most importantly, QDP successfully discriminated the serums of patients with lung diseases from those of the healthy controls based on the HNE activity determination. Overall, this study demonstrates the advantages of FRET system-based nanoprobe in imaging performance and provides an applicable tool for In Vivo HNE detection and pulmonary diseases diagnosis.
关键词： human neutrophil elastase,quantum dots,pulmonary diseases,lung cancer,fluorescence resonance energy transfer
更新于2025-09-23 15:19:57