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<em>In Vivo</em> Two-photon Imaging of Cortical Neurons in Neonatal Mice
摘要： Two-photon imaging is a powerful tool for the in vivo analysis of neuronal circuits in the mammalian brain. However, a limited number of in vivo imaging methods exist for examining the brain tissue of live newborn mammals. Herein we summarize a protocol for imaging individual cortical neurons in living neonatal mice. This protocol includes the following two methodologies: (1) the Supernova system for sparse and bright labeling of cortical neurons in the developing brain, and (2) a surgical procedure for the fragile neonatal skull. This protocol allows the observation of temporal changes of individual cortical neurites during neonatal stages with a high signal-to-noise ratio. Labeled cell-specific gene silencing and knockout can also be achieved by combining the Supernova with RNA interference and CRISPR/Cas9 gene editing systems. This protocol can, thus, be used for analyzing the developmental dynamics of cortical neurons, molecular mechanisms that control the neuronal dynamics, and changes in neuronal dynamics in disease models.
关键词： Neuroscience,in vivo imaging,single-cell labeling,mouse,cerebral cortex,Newborn,two-photon
更新于2025-09-23 15:21:01
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Unusual exophytic gingival lesion in a newborn treated with diode laser
摘要： Gingival lesions rarely occur in newborns. However, when present, they commonly worry the parents and impair the infant’s feeding, thus affecting growth. Such lesions are usually nonneoplastic in nature, although malignancies may develop; therefore, specimens must be submitted for histopathologic examination. A 2-month-old girl presented with a 10-mm nodule on the anterior lower alveolar ridge in association with natal tooth extraction and neonatal tooth eruption. The lesion was excised with high-power laser under local anesthesia, and a histopathologic diagnosis of a peripheral ossifying ?broma was made. In addition to peripheral ossifying ?bromas being rare in newborns, the use of high-power lasers for surgical procedures in newborns have been proven to be safe, comfortable, and ef?cient.
关键词： natal tooth,diode laser,neonatal tooth,newborn,Peripheral ossifying ?broma
更新于2025-09-23 15:19:57
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NIRS cerebral patterns in healthy late preterm and term infants are gender- and gestational age-dependent
摘要： Near infrared spectroscopy (NIRS) has been proposed to provide reliable information concerning brain oximetry and tissue activation level in the perinatal period. We aimed to investigate whether NIRS brain patterns in healthy preterm and term infants were gender- and gestational age-dependent. Methods: We conducted an observational study in 74 newborns, from consecutive singleton pregnancies, of whom 37 were born at term (male: n=19 female: n=18) and 37 (male: n=18 female: n=19) were preterm. Cerebral oximetry (crSO2) and fractional tissue oxygen extraction (cFTOE), were recorded on the 5th day from birth. Results: crSO2 was significantly higher and cFTOE lower (P<0.001, for both) in the preterm female than male group. At term crSO2 was significantly higher and cFTOE lower (P<0.001, for both) in males. crSO2 (male: R=0.84, P<0.001; female: R=0.74, P= P<0.001) and cFTOE (male: R=0.72, P<0.001; female: R=0.72, P<0.001) in male and female groups correlated positively with gestational age at recording. Conclusion: Different brain oximetry between males and females in preterm and term gestational ages, may suggest that in the perinatal period brain development is gender- and time-dependent. Data support the use of NIRS as a feasible tool for non-invasive cerebral monitoring.
关键词： newborn,NIRS,gender,brain development
更新于2025-09-10 09:29:36
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Comparison of Cerebral Oximeter and Pulse Oximeter Values in the first 72 hours in Premature, Asphyctic and Healthy Newborns
摘要： Aim: The monitoring of oxygenation is essential for providing patient safety and optimal results. We aimed to determine brain oxygen saturation values in healthy, asphyctic and premature newborns and to compare cerebral oximeter and pulse oximeter values in the first 72 hours of life in neonatal intensive care units. Methods : This study was conducted at the neonatal intensive care unit (NICU) of Van Yüzüncü Yil University Research and Administration Hospital. Seventy-five neonatal infants were included in the study (28 asphyxia, 24 premature and 23 mature healthy infants for control group). All infants were studied within the first 72 hours of life. We used a Somanetics 5100C cerebral oximeter (INVOS cerebral/somatic oximeter, Troy, MI, USA). The oxygen saturation information was collected by a Nellcor N-560 pulse oximeter (Nellcor-Puriton Bennet Inc, Pleasanton, CA, USA). Res ults : In the asphyxia group, the cerebral oximeter average was 76.85 ± 14.1, the pulse oximeter average was 91.86 ± 5.9 and the heart rate average was 139.91 ± 22.3. Among the premature group, the cerebral oximeter average was 79.08 ± 9.04, the pulse oximeter average was 92.01 ± 5.3 and the heart rate average was 135.35 ± 17.03. In the control group, the cerebral oximeter average was 77.56 ± 7.6, the pulse oximeter average was 92.82 ± 3.8 and the heart rate average was 127.04 ± 19.7. Conclus ion: Cerebral oximeter is a promising modality in bedside monitoring in neonatal intensive care units. It is complementary to pulse oximeter. It may be used routinely in neonatal intensive care units.
关键词： newborn,Monitoring,neonatal intensive care unit
更新于2025-09-04 15:30:14