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Turbidimetric inhibition immunoassay revisited to enhance its sensitivity via an optofluidic laser
摘要： Turbidimetric inhibition immunoassay (TIIA) is a classic immunodiagnostic method that has been extensively used for biomarker detection. However, the low sensitivity of this technique hinders its applications in the early diagnosis of diseases. Here, a new concept, optofluidic laser TIIA (OFL-TIIA), is proposed and demonstrated for sensitive protein detection. In contrast to the immunoreaction in traditional TIIA, in which the single-pass laser loss is detected, the immunoreaction in the OFL-TIIA method takes place in a laser cavity, which considerably increases the loss induced by antigen-antibody complexes (AACs) via the amplification effect of the laser. A commercial IgG TIIA kit was selected as a demonstrative model to characterize the performance of OFL-TIIA. A wide dynamic range of five orders of magnitude with an exceptional limit of detection (LOD) (1.8×10-10 g/L) was achieved. OFL-TIIA is a fast, sensitive, and low-cost immunoassay with a simple homogeneous and wash-free process and low-volume sample consumption, thus providing a new detection platform for disease diagnostics.
关键词： Biomarker detection,Optofluidic laser,Turbidimetric inhibition immunoassay,Antigen-antibody complexes,Laser dye
更新于2025-11-25 10:30:42
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DC-biased optofluidic biolaser for uric acid detection
摘要： Optofluidic biolaser is an emerging technology for chemical and biomedical sensing. However, the sensitivity of optofluidic laser is still limited by the laser threshold condition. In this paper we report a DC-biased optofluidic biolaser for uric acid (UA) detection. Inside a Fabry-Perot laser cavity, a coupled enzyme reaction was employed to transform the detection of UA into the detection of analyte-converted hydrogen peroxide (H2O2). The fluorescent product of the enzyme-catalyzed reaction, resorufin, was employed as gain medium for optofluidic lasing. An optimized concentration of H2O2 was pre-added as a DC bias to counterbalance the requirement of high analyte concentration for lasing. UA detection with a limit of detection of 3.63 μM was achieved with a low sample volume as small as 10 μl. This work provides a sensitive technology using optofluidic biolaser for the detection of substance in human body fluids that can be converted into H2O2.
关键词： Optofluidic laser,uric acid,enzyme catalytic reaction,optical biosensing
更新于2025-09-16 10:30:52
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A Fast and Reproducible ELISA Laser Platform for Ultrasensitive Protein Quantification
摘要： Optofluidic lasers are currently of high interest for sensitive, intra-cavity, biochemical analysis. In comparison with conventional methods such as fluorescence and colorimetric detection, optofluidic lasers provide a method for amplifying small concentration differences in the gain medium, thus achieving high sensitivity. Here we report the development of an on-chip ELISA (enzyme-linked immunosorbent assay) laser platform that is able to complete an assay in short amount of time with small sample/reagent volumes, large dynamic range, and high sensitivity. The arrayed micro-scale reaction wells in the ELISA lasers can be microfabricated directly on dielectric mirrors, thus significantly improving the quality of the reaction wells and detection reproducibility. The details of the fabrication and characterization of those reaction wells on the mirror are described and the ELISA laser assay protocols are developed. Finally, we applied the ELISA laser to detecting IL-6, showing that a detection limit of about 0.1 pg/mL can be achieved in 1.5 hours with 15 μL of sample/reagents per well. This work pushes the ELISA laser a step closer to solving problems in real-world biochemical analysis.
关键词： Interleukin-6,Fabry-Perot cavity,Immunoassay,Optofluidic laser,Microfabrication,ELISA
更新于2025-09-12 10:27:22