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Surveillance With PET/CT and Liquid Biopsies of Stage I-III Lung Cancer Patients After Completion of Definitive Therapy: A Randomized Controlled Trial (SUPER)
摘要： Despite increased focus on prevention as well as improved treatment possibilities, lung cancer remains among the most frequent and deadliest cancer diagnoses worldwide. Even lung cancer patients treated with curative intent have a high risk of relapse, leading to a dismal prognosis. More knowledge on the ef?cacy of surveillance with both current and new technologies as well as on the impact on patient treatment, quality of life, and survival are urgently needed. We therefore designed a randomized phase 3 trial. In one arm, every other computed tomography (CT) scan is replaced by positron emission tomography/CT, the other arm is the standard follow-up scheme with CT. The standard arm is identical to the current national Danish follow-up program. The primary endpoint is to compare the number of relapses treatable with curative intent in the 2 arms. We aim to include 750 patients over a 3-year period. Additionally, we will test the feasibility of noninvasive lung cancer diagnostics and surveillance in the form of circulating tumor DNA analysis. For this purpose, blood samples are collected before treatment and at each following control. The blood samples are stored in a biobank for later analysis and will not be used for guiding patient treatment decisions.
关键词： Liquid biopsies,NSCLC,Surveillance,QoL,PET/CT
更新于2025-09-12 10:27:22
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Nitrone-Modified Gold Nanoparticles: Synthesis, Characterization, and Their Potential as <sup>18</sup> F-Labeled Positron Emission Tomography Probes via I-SPANC
摘要： A novel bioorthogonal gold nanoparticle (AuNP) template displaying interfacial nitrone functional groups for bioorthogonal interfacial strain-promoted alkyne?nitrone cycloaddition reactions has been synthesized. These nitrone?AuNPs were characterized in detail using 1H nuclear magnetic resonance spectroscopy, transmission electron microscopy, thermogravimetric analysis, and X-ray photoelectron spectroscopy, and a nanoparticle raw formula was calculated. The ability to control the conjugation of molecules of interest at the molecular level onto the nitrone?AuNP template allowed us to create a novel methodology for the synthesis of AuNP-based radiolabeled probes.
关键词： bioorthogonal chemistry,radiolabeled probes,PET imaging,nitrone,gold nanoparticles
更新于2025-09-12 10:27:22
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Photonuclear production of medical isotopes <sup>62,64</sup> Cu using intense laser-plasma electron source
摘要： 62,64Cu are radioisotopes of medical interest that can be used for positron emission tomography (PET) imaging. Moreover, 64Cu has β? decay characteristics that allow for targeted radiotherapy of cancer. In the present work, a novel approach to experimentally demonstrate the production of 62,64Cu isotopes from photonuclear reactions is proposed in which large-current laser-based electron (e?) beams are generated from the interaction between sub-petawatt laser pulses and near-critical-density plasmas. According to simulations, at a laser intensity of 3.4 × 1021 W/cm2, a dense e? beam with a total charge of 100 nC can be produced, and this in turn produces bremsstrahlung radiation of the order of 1010 photons per laser shot, in the region of the giant dipole resonance. The bremsstrahlung radiation is guided to a natural Cu target, triggering photonuclear reactions to produce the medical isotopes 62,64Cu. An optimal target geometry is employed to maximize the photoneutron yield, and 62,64Cu with appropriate activities of 0.18 GBq and 0.06 GBq are obtained for irradiation times equal to their respective half-lives multiplied by three. The detection of the characteristic energy for the nuclear transitions of 62, 64Cu is also studied. The results of our calculations support the prospect of producing PET isotopes with gigabecquerel-level activity (equivalent to the required patient dose) using upcoming high-intensity laser facilities.
关键词： 62,64Cu,laser-plasma electron source,medical isotopes,Photonuclear production,targeted radiotherapy,PET imaging
更新于2025-09-12 10:27:22
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Evaluation of 64Cu radiolabeled anti-hPD-L1 Nb6 for positron emission tomography imaging in lung cancer tumor mice model
摘要： Recently, we selected a novel anti-hPD-L1-specific HCAb named Nb6 with high affinity (EC50= 0.65 ng/mL) for potential hPD-L1 targeted non-invasive PET imaging. In this research, Nb6 was conjugated with the bifunctional chelator NCS-Bz-NOTA ((2-[(4-Isothiocyanophenyl) methyl]-1,4,7-triazacy-clononane-1,4,7-triacetic acid)) and further labeled with radio-nuclide 64Cu. 64Cu-NOTA-Nb6 was prepared with over 95% labeling yield, over 99% radiochemical purity and 14~16 GBq/μmol specific activity after PD-10 column purification. It shows good stability in 0.01 M PBS and 5% HSA solutions. 64Cu-NOTA-Nb6 has a high binding affinity to 3.60 nM which was tested by human lung adenocarcinoma A549 cell lines. Tumor lesion can be clearly observed from 20 h to 38 h by Micro-PET equipment after 64Cu-NOTA-Nb6 administration. The study revealed that 64Cu-NOTA-Nb6 has good lesion detection ability, high ratios between tumor and non-tumor signal and can specifically target A549 xenografted tumor model. Taken together of good stability, high binding affinity, and tumor detection ability, 64Cu labeled Nb6 is a promising radio-tracer in diagnosing of hPD-L1 overexpression tumor, supposed to monitor PD-L1overexpression tumor progression and guide targeted therapy with PET molecular imaging.
关键词： Lung cancer,PET/CT imaging,64Cu-NOTA-Nb6,heavy chain-only antibody (HCAb),PD-L1
更新于2025-09-12 10:27:22
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New 55Co-labeled Albumin-Binding Folate Derivatives as Potential PET Agents for Folate Receptor Imaging
摘要： Overexpression of folate receptors (FRs) on di?erent tumor types (e.g., ovarian, lung) make FRs attractive in vivo targets for directed diagnostic/therapeutic agents. Currently, no diagnostic agent suitable for positron emission tomography (PET) has been adopted for clinical FR imaging. In this work, two 55Co-labeled albumin-binding folate derivatives-[55Co]Co-cm10 and [55Co]Co-rf42-with characteristics suitable for PET imaging have been developed and evaluated. High radiochemical yields (≥95%) and in vitro stabilities (≥93%) were achieved for both compounds, and cell assays demonstrated FR-mediated uptake. Both 55Co-labeled folate conjugates demonstrated high tumor uptake of 17% injected activity per gram of tissue (IA/g) at 4 h in biodistribution studies performed in KB tumor-bearing mice. Renal uptake was similar to other albumin-binding folate derivatives, and liver uptake was lower than that of previously reported [64Cu]Cu-rf42. Small animal PET/CT images con?rmed the biodistribution results and showed the clear delineation of FR-expressing tumors.
关键词： folate receptors,cobalt-55,PET imaging,albumin binder,ovarian cancer,folic acid
更新于2025-09-12 10:27:22
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18F-labelled triazolyl-linked argininamides targeting the neuropeptide Y Y1R for PET imaging of mammary carcinoma
摘要： Neuropeptide Y Y1 receptors (Y1R) have been found to be overexpressed in a number of different tumours, such as breast, ovarian or renal cell cancer. in mammary carcinoma the high Y1R density together with its high incidence of 85% in primary human breast cancers and 100% in breast cancer derived lymph node metastases attracted special attention. therefore, the aim of this study was the development of radioligands for Y1R imaging by positron emission tomography (pet) with a special emphasis on imaging agents with reduced lipophilicity to provide a pet ligand with improved biodistribution in comparison with previously published tracers targeting the Y1R. three new radioligands based on BIBP3226, bearing an 18F-fluoroethoxy linker (12), an 18F-PEG-linker (13) or an 18F-fluoroglycosyl moiety (11) were radiosynthesised in high radioactivity yields. The new radioligands displayed Y1R affinities of 2.8 nM (12), 29 nM (13) and 208 nM (11) and were characterised in vitro regarding binding to human breast cancer MCF-7-Y1 cells and slices of tumour xenografts. In vivo, small animal PET studies were conducted in nude mice bearing MCF-7-Y1 tumours. The binding to tumours, solid tumour slices and tumour cells correlated well with the Y1R affinities. Although 12 and 13 showed displaceable and specific binding to Y1R in vitro and in vivo, the radioligands still need to be optimised to achieve higher tumour-to-background ratios for Y1R imaging by pet. Yet the present study is another step towards an optimized pet radioligand for imaging of Y1R in vivo.
关键词： Neuropeptide Y Y1 receptors,BIBP3226,radioligands,mammary carcinoma,PET imaging
更新于2025-09-11 14:15:04
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PS02.062: CONFOCAL LASER ENDOMICROSCOPY IN THE ASSESSMENT OF PERSISTENT/RECURRENT INTESTINAL METAPLASIA/NEOPLASIA AFTER ENDOSCOPIC TREATMENT OF BORN
摘要： Recent studies revealed that membrane proteins, such as ion transporters, are specifically activated in cancer stem cells (CSCs). Therefore, these molecules are receiving a great attention as new chemotherapeutic targets of malignant tumor. This study aimed to investigate the expression and activity of ion transport-related molecules in CSCs of esophageal squamous cell carcinoma (ESCC).
关键词： Esophageal squamous cell carcinoma,FDG-PET/CT,esophageal cancer,Esophageal cancer,stem cell,TRPV2,Tranilast,False positive,LncRNA
更新于2025-09-11 14:15:04
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Comparing the Staging/Restaging Performance of 68Ga-Labeled Prostate-Specific Membrane Antigen and 18F-Choline PET/CT in Prostate Cancer
摘要： PET/CT using prostate-specific membrane antigen (PSMA) and choline radiotracers is widely used for diagnosis of prostate cancer. However, the roles of and differences in diagnostic performance between these 2 radiotracers for prostate cancer are unclear. The aim of this study was to compare the staging and restaging performance of 68Ga-labeled PSMA and 18F-choline PET/CT imaging in prostate cancer. Methods: A comprehensive search was performed in PubMed for studies reporting the staging performance of 68Ga-PSMA and 18F-choline PET/CT in prostate cancer from the inception of the database to October 1, 2018, according to the Preferred Reporting Items for Systematic Reviews and Meta-analyses statement. Thirty-five studies were included in this systematic review and meta-analysis. Pooled estimates of patient- and lesion-based sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), and diagnostic odds ratio (DOR) for 68Ga-PSMA and 18F-choline PET/CT were calculated alongside 95% confidence intervals. Summary receiver operating characteristic curves were plotted, and the area under the summary receiver operating characteristic curve (AUC) was determined alongside the Q* index. Results: The patient-based overall pooled sensitivity, specificity, PLR, NLR, DOR, and AUC of 68Ga-PSMA PET/CT for staging in prostate cancer (13 studies) were 0.92, 0.94, 7.91, 0.14, 79.04, and 0.96, respectively, whereas those of 18F-choline PET/CT (16 studies) were 0.93, 0.83, 4.98, 0.10, 68.27, and 0.95. The lesion-based overall pooled sensitivity, specificity, PLR, NLR, DOR, and AUC of 68Ga-PSMA PET/CT for staging in prostate cancer (9 studies) were 0.83, 0.95, 23.30, 0.17, 153.58, and 0.94, respectively, and those of 18F-choline PET/CT (4 studies) were 0.81, 0.92, 8.59, 0.20, 44.82, and 0.98. In both patient- and lesion-based imaging, there was no statistically significant difference in the abilities of detecting or excluding prostate cancer between 68Ga-PSMA PET/CT and 18F-choline PET/CT. Conclusions: For staging and restaging performance in patients with prostate cancer, there was no significant difference between 68Ga-PSMA PET/CT and 18F-choline PET/CT. 68Ga-PSMA PET/CT and 18F-choline PET/CT have demonstrated high diagnostic performance for accurate staging and restaging in patients with prostate cancer, and thus both should be considered for staging in this disease.
关键词： 18F-fluorocholine (18F-choline),PET,staging,prostate cancer,68Ga-PSMA CT
更新于2025-09-11 14:15:04
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Feasibility of High-Resolution PET Detector Readout by Two-Dimensional Tetra-Lateral Position-Sensitive Silicon Photomultiplier
摘要： In this paper, we present the feasibility of high-resolution PET detector readout by a two-dimensional (2D) tetra-lateral position-sensitive Silicon photomultiplier with an intrinsic cap resistive layer for charge division (CRL-SiPM). The scintillation imaging sensor for 511keV gamma ray detection is realized by coupling a 5×5 array of 0.45×0.45×6 mm3 LYSO crystals to the CRL-SiPM with an active area of 2.77×2.77 mm2 and micro APD cell pitch of ~10 μm. The scintillator arrays are clearly distinguished and good scintillator array imaging with little position distortion has been achieved. An energy resolution of ~13.5% and coincidence time resolution of 555 ps are demonstrated, respectively. Therefore, the potential PET imaging sensor with greatly reduced readout channels has been verified.
关键词： position sensitive,Silicon photomultiplier,LYSO,Avalanche photodiodes,PET detector
更新于2025-09-11 14:15:04
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In Vitro PET Imaging of a Miniature Ventricular Assist Device
摘要： Interactions between the life-sustaining ventricular assist devices and diagnostic therapies must be carefully considered to decrease the risk of inaccurate diagnostic imaging or pump failure. Methods: The MVAD? pump, currently under investigational use, was tested for interaction with radiotracers in an in vitro ?ow-loop study. The radiotracers 18F-sodium ?uoride and 18F-FDG were injected into a closed loop to determine the feasibility of direct imaging of the MVAD? pump in a PET scanner. Results: No real-time changes were observed in pump operation, and there were no statistical differences in pump parameters (power consumption, speed, and estimated ?ow rate) between the baseline and circulation conditions. In addition, no effect was observed on any external components, including the permissive-action-link controller and the batteries powering the device. Imaging of the internal pump components was possible, with obscuration observed only in the portion of the pump where the spinning impeller is located. Retention of radiotracer in the pump components after circulation was minimal (,1%). Conclusion: PET imaging is an attractive diagnostic tool for patients with a ventricular assist device and may have additional utility outside its current use, detection of infection.
关键词： ventricular assist device,PET,MVAD?,radioactive,pump
更新于2025-09-11 14:15:04