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A near-infrared fluorescence probe for ultrafast and selective detection of peroxynitrite with large Stokes shift in inflamed mouse models
摘要： Peroxynitrite (ONOO?) is a kind of reactive oxygen species (ROS) which is associated with pathogenesis of many diseases. A new near-infrared fluorescence probe (DCM-OH) which based on dicyanomethylene-4H-pyrans to detect endogenous ONOO? was designed and synthesized. The two-photon absorption cross sections and large Stokes shift make the probe deeper issue penetration and lower self-absorption. The obtained results demonstrated that probe DCM-OH could sensitively detect ONOO? with a low detection limit (53 nM). What’s more, probe DCM-OH exhibited an ultrafast response rate (within 5 s) toward ONOO?, which would be in favor of tracking the highly reactive and short-lived ONOO? in the living systems. Moreover, DCM-OH was successfully employed for imaging endogenous ONOO? in HepG2/RAW 264.7 cells and further applied to visualize oxidative stress in mouse model of inflammation.
关键词： In vivo imaging,NIR,Fluorescence probe,Peroxynitrite,Large Stokes shift
更新于2025-11-14 15:29:11
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Sensitive detection and imaging of endogenous peroxynitrite using a benzo[d]thiazole derived cyanine probe
摘要： Peroxynitrite is a short-lived endogenous reactive species and plays important roles in many physiological and pathological processes. In this work, we synthesized a near-infrared probe based on the structure of benzothiazole derived cyanine for determination of peroxynitrite (ONOO-). The designed probe specifically reacted with ONOO- through oxidative cleavage of conjugated C?C double bonds and generating the non-fluorescent product. Meanwhile, the characteristic absorption of the probe at 630 nm greatly decreased after reaction with ONOO-, accompanied by drastic color change from bright blue to green yellow, which exhibited a distinct visual feature. It was demonstrated that the probe could be used to measure ONOO- in a dose-response manner and had a detection limit lower as 26 nM. Furthermore, the probe Cy-SN was applied for the imaging of endogenous ONOO- in living cells by confocal microscopy, which showed good cell permeability and low cytotoxicity. Successful application of probe for exogenous colorimetric detection and endogenous fluorescence imaging of ONOO- is suggesting its great potential applications in biological analysis.
关键词： Colorimetric,Peroxynitrite,Benzothiazole derived cyanine,Fluorescent probe,Endogenous fluorescence imaging
更新于2025-09-23 15:23:52
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Construction of a novel far-red fluorescence light-up probe for visualizing intracellular peroxynitrite
摘要： Peroxynitrite (ONOO-) is an important reactive oxygen species (ROS), which can react with a variety of biologically active species and cause many diseases, such as cancer, neurodegenerative disorders. Herein, we develop a novel far-red fluorescent probe DCM-KA, which is equipping with α-ketoamide moiety caged DCM-NH2. The probe exhibits fluorescence off-on response to ONOO- over other biological interfering analytes by ONOO--induced α-ketoamide deprotection reaction. More importantly, the probe is biocompatible and has been successfully utilized to visualize endogenous ONOO- production in macrophage cell line J774A.1.
关键词： Fluorescence off-on,Far-red,Naked-eyes detection,Imaging endogenous peroxynitrite.,Dicyanomethylene benzopyran derivative
更新于2025-09-23 15:22:29
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A dual-channel probe with green and near-infrared fluorescence changes for in vitro and in vivo detection of peroxynitrite
摘要： In this study, a coumarin-semirhodamine hybrid based dual-channel fluorescent probe was developed for detection of peroxynitrite. This probe works well in nearly pure aqueous solution and displays a rapid, highly sensitive and selective response for peroxynitrite at two different emission wavelengths (700 and 515 nm, respectively), along with distinct color and green to near-infrared fluorescence changes. The fluorescent intensity ratio at 700 and 515 nm increases linearly with the concentration of peroxynitrite from 0 to 50 μM, and the detection limit is estimated to be 59 nM. Moreover, bioimaging of exogenous and endogenous peroxynitrite in living cells and in vivo detection of peroxynitrite with this probe at dual fluorescence channels were all successfully applied. The results suggest that this new probe can be used as a useful imaging tool for studying peroxynitrite in vitro and in vivo.
关键词： peroxynitrite,dual-channel,fluorescent probe,bioimaging,in vitro and in vivo
更新于2025-09-23 15:19:57
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Recent advances in fluorescent probes for peroxynitrite detection
摘要： Peroxynitrite (ONOO?), known as a kind of reactive nitrogen species, is a significant factor involved in a broad spectrum of physiological and pathological processes. However, their biological functions remain to be controversial or poorly characterized yet, mainly because of the lack of reliable methods for sensitive and specific detection of ONOO? in vitro and in vivo. Thus, approaches that enable noninvasive visualized imaging of ONOO? will be of enormous benefit in understanding the processes of related diseases and developing novel drugs. During the last decades, fluorescent probes, with high sensitivity and real-time spatial imaging capacity, receive more and more attention. Herein, we strive to summarize the current fluorescent probes for ONOO?, including small-molecular fluorescent probes, nanoparticles-based fluorescent probes, and genetically encoded fluorescent probes. Furthermore, the challenges and attempt to give an outlook on the possible further developments are discussed.
关键词： Fluorescent probes,Nanoparticles,Peroxynitrite,Imaging
更新于2025-09-19 17:15:36