- 标题
- 摘要
- 关键词
- 实验方案
- 产品
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Letter to the Editor: A response to Hruska’s case study on molecular breast imaging and the need for true tissue quantification
摘要： We applaud the efforts by Hruska et al. to quantify differences in tissue using molecular breast imaging (MBI) and background parenchymal uptake (BPU); we have discussed the use of such previously [2]. The approach while commendable did not provide diagnostically useful information to differentiate tissue types. This approach, like the utilization of standardized uptake value (SUV), compares differences in background with tissue [3]. As we have already discussed [2, 4, 5] in the literature, this approach is an incorrect model, due to (1) the critical lack of standardization and calibration of nuclear cameras including both SPECT/Planar and PET; (2) the utilization of ratios which are not absolute values and therefore cannot be used to differentiate tissue based upon those issues, critical to the understanding of tissue differences; and (3) the inability to truly 'measure' transitional changes in tissue, which would allow for the determination of actual treatment response on a per patient basis, saving time, money, and lives.
关键词： Patent protected,AI,Breast cancer,FMTVDM??,Theranostics,Quantification,B.E.S.T. Imaging??,Nuclear camera quantitative calibration,Breast inflammation
更新于2025-09-19 17:15:36
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Rapid monitoring of the target protein expression with a fluorescent signal based on a dicistronic construct in Escherichia coli
摘要： Real?time quantification of recombinant proteins is important in studies on fermentation engineering, cell engineering, etc. Measurement of the expression level of heterologous proteins in bacterial fermentation broth has traditionally relied on time?consuming and labor?intensive procedures, such as polyacrylamide gel electrophoresis, immunoblot analysis, and biological activity assays. We describe a simple, fast, and high sensitive assay for detecting heterologous proteins production in bacteria either at the overall level (fluorescence spectrophotometry) or at the individual level (fluorescence microscopic image) in this study. Based on a dicistronic model, the translation of target gene in the upstream open reading frame (ORF) was coupled with the synthesis of the mCherry reporter in the down?stream ORF in E. coli cells, and subsequently this demonstrated a positive correlation between the expression of target gene and mCherry. Although a time lag exists between the expression of target protein and mCherry reporter, the method described here allows facile monitoring of dynamic changes in target protein expression, relying on indirect determination of the fluorescence intensity of mCherry during fermentation in real?time models. Additionally, the performance of a single bacterial cell factory could be checked under the fluorescence microscope field.
关键词： Dicistron,Recombinant protein expression,Fluorescent signal,Quantification,Real-time
更新于2025-09-19 17:15:36
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Near-Field Radio Holography of Slant-Axis Terahertz Antennas
摘要： Principal component analysis (PCA) and independent component analysis (ICA) for radiated emissions from printed circuits are critically intercompared, revealing similarities and differences of the extracted components between both methods. The input data in this analysis are measured wideband complex-valued magnetic radiated and evanescent fields with quasi-Gaussian spatial distributions. PCA and ICA lead to similar maps of their components when considered as spatial eigenmodes, but independent components exhibit simpler field structure than principal components.
关键词： stochastic fields,principal component analysis (PCA),uncertainty quantification,Independent component analysis (ICA),radiated emissions
更新于2025-09-19 17:13:59
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[IEEE 2019 International Conference on Optical MEMS and Nanophotonics (OMN) - Daejeon, Korea (South) (2019.7.28-2019.8.1)] 2019 International Conference on Optical MEMS and Nanophotonics (OMN) - Electromagnetic FPCB micromirror scanning laser rangefinder
摘要： Since several years, the number of total hip arthroplasty revision surgeries is substantially growing. One of the main reasons for this procedure to become necessary is the loosening or damage of the prothesis, which is facilitated by bone necrosis at the implant–bone interface. Electrostimulation is one promising technique, which can accelerate the growth of bone cells and, therefore, enhance the anchorage of the implant to the bone. We present computational models of an electrostimulative total hip revision system to enhance bone regeneration. In this study, the influence of uncertainty in the conductivity of bone tissue on the electric field strength and the beneficial stimulation volume for an optimized electrode geometry and arrangement is investigated. The generalized polynomial chaos technique is used to quantify the uncertainty in the stimulation volumes with respect to the uncertain conductivity of cancellous bone, bone marrow, and bone substitute, which is used to fill defective areas. The results suggest that the overall beneficial stimulation areas are only slightly sensitive to the uncertainty in conductivity of bone tissue. However, in the proximity of tissue boundaries, larger uncertainties, especially in the transition between beneficial and understimulation areas, can be expected.
关键词： Electrical stimulation,finite-element method,multiobjective optimization,uncertainty quantification,total hip arthroplasty (THA) revision
更新于2025-09-19 17:13:59
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[IEEE 2019 IEEE 46th Photovoltaic Specialists Conference (PVSC) - Chicago, IL, USA (2019.6.16-2019.6.21)] 2019 IEEE 46th Photovoltaic Specialists Conference (PVSC) - Thin-Barrier Strained Quantum Well Superlattice Solar Cells
摘要： Cancer diagnosis is based on visual examination under a microscope of tissue sections from biopsies. But whereas pathologists rely on tissue stains to identify morphological features, automated tissue recognition using color is fraught with problems that stem from image intensity variations due to variations in tissue preparation, variations in spectral signatures of the stained tissue, spectral overlap and spatial aliasing in acquisition, and noise at image acquisition. We present a blind method for color decomposition of histological images. The method decouples intensity from color information and bases the decomposition only on the tissue absorption characteristics of each stain. By modeling the charge-coupled device sensor noise, we improve the method accuracy. We extend current linear decomposition methods to include stained tissues where one spectral signature cannot be separated from all combinations of the other tissues’ spectral signatures. We demonstrate both qualitatively and quantitatively that our method results in more accurate decompositions than methods based on non-negative matrix factorization and independent component analysis. The result is one density map for each stained tissue type that classifies portions of pixels into the correct stained tissue allowing accurate identification of morphological features that may be linked to cancer.
关键词： quantification,gastrointestinal tract,prostate,microscopy,image restoration,Blind source separation
更新于2025-09-19 17:13:59
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[IEEE 2019 IEEE 46th Photovoltaic Specialists Conference (PVSC) - Chicago, IL, USA (2019.6.16-2019.6.21)] 2019 IEEE 46th Photovoltaic Specialists Conference (PVSC) - Investigation of Accuracy of various STC Correction Procedures for I-V Characteristics of PV Modules Measured at Different Temperature and Irradiances
摘要： Principal component analysis (PCA) and independent component analysis (ICA) for radiated emissions from printed circuits are critically intercompared, revealing similarities and differences of the extracted components between both methods. The input data in this analysis are measured wideband complex-valued magnetic radiated and evanescent fields with quasi-Gaussian spatial distributions. PCA and ICA lead to similar maps of their components when considered as spatial eigenmodes, but independent components exhibit simpler field structure than principal components.
关键词： Independent component analysis (ICA),principal component analysis (PCA),radiated emissions,uncertainty quantification,stochastic fields
更新于2025-09-19 17:13:59
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Molecular Technology (Life Innovation) || Self-assembled Monolayers from Carbon-Based Ligands on Metal Surfaces
摘要： Rift Valley fever virus (RVFV) is an arthropod-borne, zoonotic disease endemic to sub-Saharan Africa and the Arabian Peninsula. Outbreaks of Rift Valley fever have had up to 100% mortality rates in fetal and neonatal sheep. Upon infection of ruminant and human hosts alike, RVFV infection causes an at times severe hepatitis and pathology in many other organs. The enveloped virion contains a tripartite, predominantly negative-sense, single-stranded RNA genome, which codes for the proteins the virus needs to replicate both in mammalian hosts and insect vectors. Endemic countries often use attenuated RVFV strains for vaccination of livestock but there are no commercially licensed vaccines for humans or livestock in non-endemic areas. In the laboratory, RVFV can be readily propagated and manipulated in vitro using cell culture systems. Presented in this article are techniques routinely used in RVFV research that have proven successful in our laboratories.
关键词： Rift Valley fever virus,plaque assay,TCID50,virology,bunyaviruses,focus-forming assay,virus quantification,propagation
更新于2025-09-19 17:13:59
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[IEEE 2019 21st International Middle East Power Systems Conference (MEPCON) - Cairo, Egypt (2019.12.17-2019.12.19)] 2019 21st International Middle East Power Systems Conference (MEPCON) - Optimal Allocation of Reactive Power Compensation in a Distribution Network with Photovoltaic System Integration
摘要： Since several years, the number of total hip arthroplasty revision surgeries is substantially growing. One of the main reasons for this procedure to become necessary is the loosening or damage of the prothesis, which is facilitated by bone necrosis at the implant–bone interface. Electrostimulation is one promising technique, which can accelerate the growth of bone cells and, therefore, enhance the anchorage of the implant to the bone. We present computational models of an electrostimulative total hip revision system to enhance bone regeneration. In this study, the influence of uncertainty in the conductivity of bone tissue on the electric field strength and the beneficial stimulation volume for an optimized electrode geometry and arrangement is investigated. The generalized polynomial chaos technique is used to quantify the uncertainty in the stimulation volumes with respect to the uncertain conductivity of cancellous bone, bone marrow, and bone substitute, which is used to fill defective areas. The results suggest that the overall beneficial stimulation areas are only slightly sensitive to the uncertainty in conductivity of bone tissue. However, in the proximity of tissue boundaries, larger uncertainties, especially in the transition between beneficial and understimulation areas, can be expected.
关键词： Electrical stimulation,finite-element method,multiobjective optimization,uncertainty quantification,total hip arthroplasty (THA) revision
更新于2025-09-19 17:13:59
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An LED-Driven AuNPs-PDMS Microfluidic Chip and Integrated Device for the Detection of Digital Loop-Mediated Isothermal DNA Amplification
摘要： The sensitive quantification of low-abundance nucleic acids holds importance for a range of clinical applications and biological studies. In this study, we describe a facile microfluidic chip for absolute DNA quantifications based on the digital loop-mediated isothermal amplification (digital LAMP) method. This microfluidic chip integrates a cross-flow channel for droplet generation with a micro-cavity for droplet tiling. DNA templates in the LAMP reagent were divided into ~20,000 water-in-oil droplets at the cross-flow channel. The droplets were then tiled in the micro-cavity for isothermal amplification and fluorescent detection. Different from the existing polydimethylsiloxane (PDMS) microfluidic chips, this study incorporates gold nanoparticles (AuNPs) into PDMS substrate through silica coating and dodecanol modification. The digital LAMP chip prepared by AuNPs-PDMS combines the benefits of the microstructure manufacturing performance of PDMS with the light-to-heat conversion advantages of AuNPs. Upon illumination with a near infrared (NIR) LED, the droplets were stably and efficiently heated by the AuNPs in PDMS. We further introduce an integrated device with a NIR heating unit and a fluorescent detection unit. The system could detect HBV (hepatitis B virus)-DNA at a concentration of 1 × 101 to 1 × 104 copies/μL. The LED-driven digital LAMP chip and the integrated device; therefore, demonstrate high accuracy and excellent performance for the absolute quantification of low-abundance nucleic acids, showing the advantages of integration, miniaturization, cost, and power consumption.
关键词： digital LAMP,gold nanoparticles,integrated device,DNA quantification
更新于2025-09-19 17:13:59
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Simultaneous Quantification of Two Flavonoids in <i>Morus alba</i> by High Performance Liquid Chromatography Coupled with Photodiode Array Detector
摘要： The root bark of Morus alba L. (Family: Moraceae) is an important medicinal herb in many countries and has long been used as a traditional medicine for the treatment of cough, fever, blood pressure reduction, and respiratory diseases. In the present study, the simultaneous determination of two flavonoids, kuwanon G and morusin, for quality control of M. alba was conducted using high-performance liquid chromatography (HPLC) equipped with photodiode array (PDA) detector. The column used for separation of kuwanon G and morusin was a Gemini C18 analytical column maintained at 45°C. The mobile phase for efficient separation of two analytes was flowed 0.1% (v/v) aqueous formic acid–acetonitrile with gradient elution. The detection wavelength for quantification was set at 266 nm. The optimized method showed good linearity with coefficients of determination of 0.9998 within the tested concentration ranges. The limits of detection for the two flavonoids, kuwanon G and morusin, were 0.69 μg/mL and 0.35 μg/mL and the limits of quantification of kuwanon G and morusin, were 2.10 μg/mL and 1.07 μg/mL. The recoveries were 98.40–111.55% and the relative standard deviation (RSD) value was within 3.50%. The RSD values of intra- and interday precisions were 0.08–0.70% and 0.06–0.48%, respectively. The amounts of kuwanon G and morusin were 1.94–2.26 mg/g and 1.05–1.12 mg/g. The established HPLC–PDA method will help to improve the quality control of M. alba and related products.
关键词： Simultaneous quantification,Morus alba,HPLC–PDA,Flavonoids
更新于2025-09-16 10:30:52