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Development of Photo-Activated ROS-Responsive Nanoplatform as a Dual-Functional Drug Carrier in Combinational Chemo-Photodynamic Therapy
摘要： Dual functional drug carrier has been a modern strategy in cancer therapy because it is a platform to elicit additive and synergistic effects through combination therapy. Photo-activated external stimuli such as reactive oxygen species (ROS) also ensure adequate drug delivery in a precise temporal and spatial manner. However, current ROS-responsive drug delivery systems usually require tedious synthetic procedures. A facile one-pot approach has been reported herein, to obtain self-assembled polymeric nanocarriers (NCs) for simultaneous paclitaxel (PTX)- and Rose Bengal (RB)-loading to achieve combined chemo-photodynamic therapy and controlled drug release in responsive to a light-induced ROS stimulus. To encapsulate these hydrophobic and hydrophilic drugs, chitosan (CTS), branched polyethylenimine (bPEI) and polyvinyl alcohol (PVA) were selected and fabricated into nanoblended matrices through an oil-in-water emulsion method. The amphiphilic properties of CTS permit simultaneous entrapment of PTX and RB, while the encapsulation efficiency of RB was further improved by increasing the amount of short-chain bPEI. During the one-step assembly process, bovine serum albumin (BSA) was also added to condense the cationic tripolymer mixtures into more stable nanocarriers (BNCs). Hyaluronic acid (HA) was subsequently grafted onto the surface of BNCs through electrostatic interaction, leading to the formation of HA-BSA/CTS/PVA/bPEI-blended nanocarriers (HBNCs) to achieve an efficient prostate-cancer-cell uptake. Importantly, in response to external light irradiation, HBNCs become destabilized owing to the RB-mediated photodynamic action. It allows an on-demand dual-payload release to evoke a simultaneous photodynamic and chemo treatment for cancer cell eradication. Thus, HBNCs present a new promising approach that exhibits a specific vulnerability to RB-induced photosensitization. The consequent dual-cargo release is also expected to successfully combat cancer through a synergistic anti-tumor effect.
关键词： ROS-responsive,photodynamic therapy,nanocarrier,chemotherapy,combined therapy
更新于2025-09-23 15:23:52
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Photodynamic therapy versus systemic antibiotic for the treatment of periodontitis in a rat model
摘要： Backgroup: To compare the therapeutic effect of photodynamic therapy (PDT) with Toluidine blue O hydrogel versus systemic antibiotic (SA) in treating periodontitis on rats. Methods: Thirty-two Wistar rats were divided into 4 groups and treated differently: Negative Control (NC) group, normal rats; Positive Control (PC) group, rats with periodontitis; SA group, rats with periodontitis treated with systemic antibiotic; PDT group, rats with periodontitis treated with PDT. After treatment, Gingival sulcus bacterial load was measured by counting the colony forming units per milliliter (CFU ml-1). The tooth and periodontal tissues were histologically processed to analyze histological and immunohistochemical profile. Gingival samples were obtained to quantify interleukyn-1 beta (IL-1β) and tumor necrosis factor-alpha (TNF-α) levels. Results: Gingival sulcus bacteria load is significantly lower in PDT group compared to the SA group. The histological analysis showed that some extremely effective repair signs of periodontal tissue were presented in PDT group, such as no periodontal pocket, no bone resorption, few inflammatory cells, massive fibroblasts and collagen fibers. Several effective repair signs of periodontal tissue were also observed in SA group, such as shallow periodontal pocket, small amount of inflammatory cells, substantial fibroblasts and collagen fibers. There were lower COX-2, MMP-8 and RANK immunolabeling, higher OPG immunolabeling in PDT group compared with SA group. The IL-1β and TNF-α levels in PDT group were lower than those in NC group, but higher than those in SA group. Conclusions: PDT was effective to treat experimental periodontitis and was superior to systemic metronidazole as a treatment for periodontitis.
关键词： Cytokine(s),Reactive oxygen species (ROS),Antimicrobial(s),Periodontitis
更新于2025-09-23 15:23:52
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ROS dependent antitumour activity of photo-activated iron(III) complexes of amino acids
摘要： Several amino acid-based photo-active monomeric iron(III) complexes of the general formula, [Fe(L)2]?, where L = Schiff base ligands (salisalidene arginine, salicylidenetryptophan, 3,5-di-tert-butyl benzalidine arginine and salicylidene tryptophan) were synthesized, characterized and explored for photo-activated anticancer activity to Chang Liver Cells, HeLa and MCF-7 cells. Complexes exhibited remarkable photo-cytotoxicity with IC50 value to the extent of 0.7 μM to Chang Liver Cells in visible light and there was a 40-fold enhancement in cytotoxicity in comparison to the cytotoxicity in dark. Complexes were non-toxic to MCF-10A (normal cells) in dark and visible light (IC50 > 100 μM in dark; IC50 > 80 μM in visible light) signifying target-speci?c nature of the anti-tumour activity of the complexes. Increased ROS concentration, as probed by DCFDA assay, in the cancer cells was responsible for apoptotic cell death. Decarboxylation or phenolate-Fe(III) charge transfer of photo-activated iron(III) complexes generating ?OH radicals (ROS) were responsible for the apoptosis. Overall, the tumour-selective photo-activated anticancer activity of the amino acid-based iron(III) complexes have shown a promising aspect in developing iron-based photo-chemotherapeutics as the next generation PDT agents.
关键词： ROS generation,photo-activation,Iron(III) complexes,amino acid,photocytotoxicity
更新于2025-09-23 15:23:52
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[IEEE 2018 15th International Conference on Control, Automation, Robotics and Vision (ICARCV) - Singapore, Singapore (2018.11.18-2018.11.21)] 2018 15th International Conference on Control, Automation, Robotics and Vision (ICARCV) - Map Comparison of Lidar-based 2D SLAM Algorithms Using Precise Ground Truth
摘要： This paper presents a comparative analysis of three most common ROS-based 2D Simultaneous Localization and Mapping (SLAM) libraries: Google Cartographer, Gmapping and Hector SLAM, using a metric of average distance to the nearest neighbor (ADNN). Each library was applied to construct a map using data from 2D lidar that was placed on an autonomous mobile robot. All the approaches have been evaluated and compared in terms of inaccuracy constructed maps against the precise ground truth presented by FARO laser tracker in static indoor environment.
关键词： ground truth,ADNN,lidar,ROS,map comparison,SLAM
更新于2025-09-23 15:23:52
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Interference-free surface-enhanced Raman scattering nanosensor for imaging and dynamic monitoring of reactive oxygen species in mitochondria during photothermal therapy
摘要： Highly reliable detection, imaging, and monitoring of reactive oxygen species (ROS) at subcellular organelles are critical for understanding the biological roles of ROS and learning the pathogenesis of some diseases. In this study, we presented an interference-free surface-enhanced Raman scattering (SERS)-active nanoprobe for the intracellular ROS detection. This nanoprobe was designed as an Au core-Ag shell nanoparticle (Au@Ag NPs) with a SERS reporter (4-mercaptobenzonitrile) resided in the inner of the core-shell. Intracellular ROS is able to etch the Ag shell and dramatically decreases the SERS intensity of the SERS reporters. It is worth mentioning that the Raman band of the reporter we used in this study locate in the cellular Raman-silent region (1800?2800 cm?1), which eliminates interference possibility from cellular molecules. The shell can also further protect the reporters from the interference of mediums. We detected ROS at subcellular organelle level, e.g. mitochondria, by modifying the surface of the nanoprobes with a mitochondria-targeting peptide. And for the first time, the SERS-based monitoring of ROS at mitochondria during the photothermal therapy process was validated and the profiles of the ROS distribution were disclosed by SERS mapping.
关键词： ROS,mitochondria,SERS,PTT,interference-free
更新于2025-09-23 15:23:52
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ROS mediated antibacterial activity of photoilluminated riboflavin: A photodynamic mechanism against nosocomial infections
摘要： Nosocomial infections are a major threat to modern therapeutics. The major causative agent of these infections is multidrug-resistant gram-negative bacteria, which impart high morbidity and mortality rate. This has led to an urge for the development of new antibiotics. Antimicrobial photodynamic therapy is a promising strategy to which till date no resistant strain has been reported. Since the efficacy of photodynamic therapy largely depends on the selection and administration of an appropriate photosensitizer, therefore, the realization of clinically active photosensitizers is an immediate need. Here, by using E. coli as a study model we have demonstrated the antimicrobial photodynamic potential of riboflavin. Intracellular ROS formation by DCFH-DA assay, lipid peroxidation, protein carbonylation, LDH activity was measured in treated bacterial samples. Enzymatic (SOD, CAT, GSH) antioxidants and non-enzymatic (GSH) was further evaluated. Bacterial death was confirmed by colony forming assay, optical microscopy and scanning electron microscopy. The treated bacterial cells exhibited abundant ROS generation and marked increment in the level of oxidative stress markers as well as significant reduction in LDH activity. Marked reduction in colony forming units was also observed. Optical microscopic and SEM images further confirmed the bacterial death. Thus, we can say that photoilluminated riboflavin renders the redox status of bacterial cells into a compromised state leading to significant membrane damage ultimately causing bacterial death. This study aims to add one more therapeutic dimension to photoilluminated riboflavin as it can be effectively employed in targeting bacterial biofilms occurring on hospital wares causing several serious medical conditions.
关键词： ROS,Antibacterial,Riboflavin,Photodynamic therapy,Nosocomial infection
更新于2025-09-23 15:23:52
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Increased resistance of <i>Salmonella</i> Typhimurium and <i>Escherichia coli</i> O157:H7 to 222-nm krypton-chlorine excilamp treatment by acid adaptation
摘要： In this study, we examined the change in resistance of Salmonella Typhimurium and Escherichia coli O157:H7 to 222-nm krypton-chlorine (KrCl) excilamp treatment as influenced by acid adaptation, and identified a mechanism of resistance change. In addition, changes in apple juice quality indicators such as color, total phenols and DPPH free radical scavenging activity during treatment were measured. Acid adapted- and non-acid adapted pathogens were induced by growing the cells in TSB without dextrose (TSB w/o D) at pH 7.3 and TSB w/o D at pH 5.0 adjusted with HCl, respectively. For the KrCl excilamp treatment, acid-adapted pathogens exhibited significantly (P < 0.05) higher D5d values, which indicate dosages required for achieving 5-log reduction, than non-acid adapted pathogens in both commercially clarified apple juice and PBS, and the pathogens in the juice showed significantly (P < 0.05) higher D5d values than those in PBS because of the UV-absorbing characteristics of apple juice. Through mechanism identification, it was found that the generation of lipid peroxidation in cell membrane, inducing cell membrane destruction, of acid adapted cells was significantly (P < 0.05) less than that of non-acid adapted cells for the same amount of reactive oxygen species (ROS) generated at the same dose because the ratio of unsaturated to saturated fatty acids (USFA/SFA) in the cell membrane was significantly (P < 0.05) decreased as a result of acid adaptation. Treated apple juice showed no significant (P > 0.05) differences in quality indicators compared to untreated controls during the treatment of 1773 mJ/cm2.
关键词： acid adaptation,ROS,E. coli O157:H7,apple juice,cell membrane fatty acid,ultraviolet irradiation,S. Typhimurium,222-nm KrCl excilamp
更新于2025-09-23 15:22:29
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Poly(dopamine) and Ag nanoparticle-loaded TiO <sub/>2</sub> nanotubes with optimized antibacterial and ROS-scavenging bioactivities
摘要： Aim: To create polydopamine (PDA) and Ag nanoparticle-loaded TiO2 nanotubes coating on titanium (Ti) alloy. Materials & methods: TiO2-PDA-Ag coating was fabricated on Ti implants by electrochemical anodization. The in vitro and in vivo bactericidal and antibiofilm activities were tested. Intracellular reactive oxygen species (ROS) and antioxidative capability were measured, and cell proliferation, adhesion and cell morphology were characterized. Results: TiO2-PDA-Ag coating showed satisfactory bactericidal and antibiofilm activities in vitro and in vivo, improved Ag release pattern, evident ROS scavenging properties and enhanced cell adhesion and proliferation. Conclusion: Our study successfully fabricated a PDA and Ag nanoparticle-loaded TiO2 nanotubes coating on Ti alloy. The improved Ag release kinetics and ROS-scavenging properties achieve an optimal balance between antibacterial ability and biocompatibility.
关键词： antibacterial,nanotubular structures,enhanced adhesion,ROS scavenging,poly(dopamine)
更新于2025-09-23 15:22:29
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Vision-based people detection using depth information for social robots
摘要： Robots are starting to be applied in areas which involve sharing space with humans. In particular, social robots and people will coexist closely because the former are intended to interact with the latter. In this context, it is crucial that robots are aware of the presence of people around them. Traditionally, people detection has been performed using a flow of two-dimensional images. However, in nature, animals’ sight perceives their surroundings using color and depth information. In this work, we present new people detectors that make use of the data provided by depth sensors and red-green-blue images to deal with the characteristics of human–robot interaction scenarios. These people detectors are based on previous works using two-dimensional images and existing people detectors from different areas. The disparity of the input and output data used by these types of algorithms usually complicates their integration into robot control architectures. We propose a common interface that can be used by any people detector, resulting in numerous advantages. Several people detectors using depth information and the common interface have been implemented and evaluated. The results show a great diversity among the different algorithms. Each one has a particular domain of use, which is reflected in the results. A clever combination of several algorithms appears as a promising solution to achieve a flexible, reliable people detector.
关键词： kinect,people detection,benchmarking,depth image processing,depth sensor,Social robotics,user detection,ROS
更新于2025-09-23 15:22:29
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Sensors Based on Metal Nanoclusters Stabilized on Designed Proteins
摘要： Among all new nanomaterials, metal nanoclusters (NCs) have attracted special attention due to their interesting optical properties, among others. Metal NCs have been recently studied and used as sensors for different analytes. However, there is a need to explore the potential of these new sensors in a systematic manner and to develop new systems to broaden the possibilities that sensing offers to the industry. In this work, we show the potential use of repeat protein scaffolds as versatile templates for the synthesis and stabilization of various metal NCs, speci?cally Au, Ag, and CuNCs. The resulting protein-metal NCs hybrids are evaluated as sensors for different stimuli such as temperature, ions, or reactive oxygen species (ROS). Among the three protein-metal NCs, all performed nicely as temperature sensors, AuNCs responded to metal ions, and AgNCs were able to detect ROS.
关键词： nanosensor,ROS sensors,metal nanocluster,protein design,temperature sensor,metal sensors,?uorescent probe
更新于2025-09-23 15:21:21