- 标题
- 摘要
- 关键词
- 实验方案
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Assessing retinal ganglion cell death and neuroprotective agents using real time imaging
摘要： The evaluation of retinal ganglion cell (RGC) death is a key part of retinal disease care. Previously, we used a Sytox Orange (SO)-based real-time imaging method to assess the RGCs in mice that underwent optic nerve crush. Here, we used N-methyl-D-aspartate (NMDA) injury in rats to confirm our model and assess the effect of neuroprotective agents on RGCs. The rats received NMDA injury and the intravitreal injection of SO, a cell-impermeant dyeing compound that targets nucleic acid. After ten minutes, non-invasive confocal scanning laser ophthalmoscopy visualized damaged or dying cells. Finally, the retinas were flat-mounted for histological confirmation of RGC death, with retrograde Fluorogold labeling and Alexa Fluor 488 Annexin V-conjugate (Annexin V) staining. This also revealed the time course of retinal cell death and the neuroprotective effect of SNJ-1945. Real-time imaging showed that SO-positive cells significantly increased starting 2 hours after NMDA injection and reached an approximate plateau at 3 hours. SO-positive cells were positive for Fluorogold and Annexin V in the isolated retinas. Moreover, the number of SO-positive retinal cells was significantly lower after treatment with SNJ-1945, compared to carboxymethyl cellulose. These results were confirmed in the isolated retinas. Thus, real-time imaging with SO allows the quick quantification of NMDA-induced RGC damage and death, and evaluation of neuroprotective agents. This technique may aid research into the development of new neuroprotective therapies.
关键词： retinal ganglion cell,Real-time imaging,SYTOX orange,neuroprotection
更新于2025-11-21 11:24:58
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Freezing Induced Turn-on Modality for Real-time Imaging in Cryosurgery
摘要： Cryosurgery has attracted great attention for the treatment of tumors due to its obvious advantages. However, it still remains a challenge to determine the volume of frozen tissues in real-time, which greatly lowers the therapeutic efficacy of cryosurgery and hinders its broad application for the treatment of cancers. Herein, we report a freezing induced turn-on strategy for selective real-time imaging of frozen cancer cells. As a type of aggregation induced emission (AIE) fluorogen, TABD-Py molecules interact specifically with ice crystals and form aggregates at the ice/water interface. Consequently, bright fluorescent emission appears upon freezing. Note that TABD-Py molecules are enriched only in the cancer cells and exhibit high biocompatibility as well as low cytotoxicity, therefore a freezing induced turn-on imaging modality for cryosurgery is developed, which will certainly maximize therapeutic efficacy of cryosurgery in treating tumors.
关键词： aggregation induced emission,cancer cells,real-time imaging,freezing,cryosurgery
更新于2025-09-23 15:23:52
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THROUGH THE LOOKING GLASS: Real-Time Imaging in Brachypodium Roots and Osmotic Stress Analysis
摘要： To elucidate dynamic developmental processes in plants, live tissues and organs must be visualised frequently and for extended periods. The development of roots is studied at a cellular resolution not only to comprehend the basic processes fundamental to maintenance and pattern formation but also study stress tolerance adaptation in plants. Despite technological advancements, maintaining continuous access to samples and simultaneously preserving their morphological structures and physiological conditions without causing damage presents hindrances in the measurement, visualisation and analyses of growing organs including plant roots. We propose a preliminary system which integrates the optical real-time visualisation through light microscopy with a liquid culture which enables us to image at the tissue and cellular level horizontally growing Brachypodium roots every few minutes and up to 24 h. We describe a simple setup which can be used to track the growth of the root as it grows including the root tip growth and osmotic stress dynamics. We demonstrate the system’s capability to scale down the PEG-mediated osmotic stress analysis and collected data on gene expression under osmotic stress.
关键词： root,real-time imaging,osmotic stress,Casparian bands,Brachypodium,PDMS,neutral red,PEG-6000
更新于2025-09-23 15:22:29
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Unraveling the Safety Profile of Nanoscale Particles and Materials - From Biomedical to Environmental Applications || Applications of Fluorescent Quantum Dots for Reproductive Medicine and Disease Detection
摘要： Understanding the mechanisms associated with fertility and disease management in animals remains challenging. Continuing advances in nanotechnology provide new tools and alternative approaches for the investigation of these mechanisms. Fluorescent quantum dot nanoparticles, for example, have unique physicochemical properties, which allow for in vivo and in vitro imaging in various areas of life sciences. Traditional quantum dots contain heavy metal semiconductor cores, which have raised concern over their potential for toxicity. The majority of available quantum dots today prevent heavy metal release with additional chemical and polymer layers for safe water solubility. In this chapter, the most widely used quantum dots made of cadmium selenide, which possess great potential for real-time imaging in disease detection and reproductive medicine, are discussed.
关键词： real-time imaging,spermatozoa,quantum dots,in vivo imaging,fertility,luminescence
更新于2025-09-23 15:21:01
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Interventional Oncology (Principles and Practice of Image-Guided Cancer Therapy) || Imaging in interventional oncology: Role of image guidance
摘要： Advances in medical imaging have created the opportunity for minimally invasive, image-guided oncologic care by allowing: (1) procedure planning; (2) device delivery; (3) intraprocedure monitoring; and (4) therapy assessment. Although most current image-guided therapy still utilizes standard diagnostic imaging equipment, interventional use of imaging equipment has in fact different priorities compared with diagnostic uses of such equipment. Therefore, interventional procedures prioritize imaging equipment that: (1) provides real-time imaging; (2) lowers radiation dose; and (3) provides greater physician access to the patient. In contrast to diagnostic imaging, lower image quality is an acceptable compromise for real-time imaging for interventional procedures. Patients have already undergone high-quality diagnostic imaging when they are referred to interventional therapies. Moreover, high-quality diagnostic imaging may require more time and more radiation dose than fast imaging of a restricted region of interest as performed for image guidance of interventions.
关键词： image-guided therapy,real-time imaging,physician access,medical imaging,radiation dose,interventional oncology
更新于2025-09-23 15:21:01
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100 GHz Multiple Colliding Pulse Generation From Cleaved Facet-Free Multi-Section Semiconductor Laser Diode
摘要： Terahertz (THz) cameras comprising an uncooled micro-bolometer array have been developed for simple THz imaging, and the improvement of their sensitivity is one of the important issues. We fabricated a new micro-bolometer array with a resonant cavity structure for a real-time THz camera, alongside a new method for evaluating the sensitivity across a wide range of the THz frequency region. The frequency dependence of the sensitivity of the THz camera is measured in the 0.5–2.0-THz frequency range taking the polarization dependence into account. It was found that the resonant cavity structure effectively increased the sensitivity of the THz camera, and, actually, the improvement by one order of magnitude was achieved in the frequency range below 1 THz. The THz camera with much enhanced sensitivity will expand the frontiers of real-time THz imaging such as molecular imaging and nondestructive inspection.
关键词： focal plane array,micro-bolometer,terahertz (THz),real-time imaging,Compact terahertz camera
更新于2025-09-23 15:19:57
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Sectioning-based ADMM Imaging for Fast Node Communication with a Compressive Antenna
摘要： In this paper, a novel norm-1 regularized imaging technique, based on the Alternating Direction Method of Multipliers (ADMM) algorithm, is presented. This technique divides the sensing matrix of the imaging system in submatrices by columns. This method is capable of imaging metallic targets with a reduced amount of measurements, by sectioning the imaging domain into several regions and optimizing them individually in different nodes. This technique reduces the amount of information to be shared among the nodes, compared to the consensus ADMM that divides the sensing matrix into submatrices by rows. This makes the communication among the nodes faster for large imaging domains that need to be distributed into several computational nodes of a cluster. A Compressive Reflector Antenna (CRA) has been recently proposed as a low cost hardware to provide high sensing capacity. The use of the column-wise division ADMM algorithm in combination with the imaging capabilities of the CRA allows a distributed and quasi real-time imaging, while reducing the communications among the computational nodes.
关键词： Compressive antenna,nodes communication,distributed ADMM,real-time imaging,norm-1 regularization
更新于2025-09-19 17:15:36
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Generation of differential topographic images for surface inspection of long products
摘要： The current manufacturing industries need efficient quality control systems to ensure their products are free of defects. In most cases, surface inspection is carried out by automatic systems that process 2D images which lack measurable information such as the height or depth of the surface defects. An alternative technology for surface inspection is laser scanning. Using this technique, a 3D representation of a product can be generated and therefore, defects can be easily measured. This paper proposes a real-time algorithm to generate differential topographic images of the surface of a product using laser scanning. The images generated by the proposed method are a flattened representation of the surface of the product which compare it to a perfect-shaped product. In these images, the volumetric defects can be easily segmented and measured using computer vision techniques to fulfill the requirements of the international standards of quality. The proposed algorithm is tested on 500,000 profiles meeting the constraints of real time.
关键词： 3D reconstruction,Real-time imaging,Differential image,Laser scanning
更新于2025-09-19 17:15:36
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Gold Nanoparticle-Based Fluorescent Theranostics for Real-Time Image-Guided Assessment of DNA Damage and Repair
摘要： Chemotherapeutic dosing, is largely based on the tolerance levels of toxicity today. Molecular imaging strategies can be leveraged to quantify DNA cytotoxicity and thereby serve as a theranostic tool to improve the efficacy of treatments. Methoxyamine-modified cyanine-7 (Cy7MX) is a molecular probe which binds to apurinic/apyrimidinic (AP)-sites, inhibiting DNA-repair mechanisms implicated by cytotoxic chemotherapies. Herein, we loaded (Cy7MX) onto polyethylene glycol-coated gold nanoparticles (AuNP) to selectively and stably deliver the molecular probe intravenously to tumors. We optimized the properties of Cy7MX-loaded AuNPs using optical spectroscopy and tested the delivery mechanism and binding affinity using the DLD1 colon cancer cell line in vitro. A 10:1 ratio of Cy7MX-AuNPs demonstrated a strong AP site-specific binding and the cumulative release profile demonstrated 97% release within 12 min from a polar to a nonpolar environment. We further demonstrated targeted delivery using imaging and biodistribution studies in vivo in an xenografted mouse model. This work lays a foundation for the development of real-time molecular imaging techniques that are poised to yield quantitative measures of the efficacy and temporal profile of cytotoxic chemotherapies.
关键词： Cy7MX-loaded gold nanoparticles,DNA repair mechanism,AP-targeted delivery,chemotheranostic,molecular probe,real-time imaging
更新于2025-09-19 17:15:36
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[IEEE 2019 IEEE 2nd International Conference on Information Communication and Signal Processing (ICICSP) - Weihai, China (2019.9.28-2019.9.30)] 2019 IEEE 2nd International Conference on Information Communication and Signal Processing (ICICSP) - Vision-based Automatic Detection of Suspended Solids in Bottled Mineral Water
摘要： Terahertz (THz) cameras comprising an uncooled micro-bolometer array have been developed for simple THz imaging, and the improvement of their sensitivity is one of the important issues. We fabricated a new micro-bolometer array with a resonant cavity structure for a real-time THz camera, alongside a new method for evaluating the sensitivity across a wide range of the THz frequency region. The frequency dependence of the sensitivity of the THz camera is measured in the 0.5–2.0-THz frequency range taking the polarization dependence into account. It was found that the resonant cavity structure effectively increased the sensitivity of the THz camera, and, actually, the improvement by one order of magnitude was achieved in the frequency range below 1 THz. The THz camera with much enhanced sensitivity will expand the frontiers of real-time THz imaging such as molecular imaging and nondestructive inspection.
关键词： focal plane array,micro-bolometer,terahertz (THz),real-time imaging,Compact terahertz camera
更新于2025-09-19 17:13:59