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EXPRESS: Cyclic Changes in the Amide Bands Within <i>Escherichia coli</i> Biofilms Monitored Using Real-Time Infrared Attenuated Total Reflection Spectroscopy
摘要： Contrary to the planktonic state of bacteria, their biofilm form represents severe complications in areas such as human medicine or food industry due to the increasing resistance against harsh conditions and treatment. In the present study, infrared attenuated total reflection (IR-ATR) spectroscopy has been applied as an analytic tool studying Escherichia coli (E. coli) biofilm formation close to real time. We report on IR spectroscopic investigations on the biofilm formation via ATR waveguides probing the biofilm in the spectral window of 1800–900 cm?1 at dynamic flow conditions, which facilitated monitoring the growth dynamics during several days. Key IR bands are in the range 1700–1590 cm?1 (amide I), 1580–1490 cm?1 (amide II), and 1141–1006 cm?1 extracellular polymeric substances (EPS), which were evaluated as a function of time. Cyclic fluctuations of the amide I and amide II bands and a continuous increase of the EPS band were related to the starvation of bottom-layered bacteria caused by the nutrient gradient. Potential death of bacteria may then result in cannibalistic behavior known for E. coli colonies. Observing this behavior via IR spectroscopy allows revealing these cyclical changes in bottom-layered bacteria within the biofilm under continuous nutrient flow, in molecular detail, and during extended periods for the first time.
关键词： bacteria,starvation,Escherichia coli,EPS,IR-ATR spectroscopy,amide band,biofilm,infrared attenuated total reflectance
更新于2025-09-19 17:15:36
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Interaction of Zinc Oxide and Copper Oxide Nanoparticles with Chlorophyll: A Fluorescence Quenching Study
摘要： The present study aims to investigate the interactions of zinc oxide nanoparticles and copper oxide nanoparticles with the major photosynthetic pigment chlorophyll using ultraviolet-visible, steady state, and time resolved laser induced fluorescence spectroscopy. The steady state fluorescence measurements show that zinc oxide and copper oxide nanoparticles quench the fluorescence of chlorophyll in concentration-dependent manner. The Stern-Volmer plot for the chlorophyll-zinc oxide nanoparticles is linear, and the value of quenching constant has been observed to increase with temperature indicating the possibility of dynamic quenching. A decrease in the lifetime of chlorophyll with increase in the concentration of zinc oxide nanoparticles confirms the involvement of dynamic quenching in the chlorophyll–zinc oxide nanoparticle interaction. In the case of copper oxide nanoparticles, the Stern-Volmer plot deviates from linearity observed in the form of upward curvature depicting the presence of both static and dynamic quenching. In addition, the lifetime of chlorophyll decreases with increase in the concentration of copper oxide nanoparticles displaying the dominance of dynamic quenching in the chlorophyll-copper oxide nanoparticle interaction. The decrease observed in the value of binding constant with increasing temperature and negative values of change in enthalpy, entropy, and Gibb’s free energy indicates that van der Waal and hydrogen bonding are the prominent forces during the interaction of chlorophyll with both zinc oxide and copper oxide nanoparticles and that the process is spontaneous and exothermic. The interaction of zinc oxide and copper oxide nanoparticles with chlorophyll occurs through electron transfer mechanism. The obtained results are useful in understanding the sensitization processes involving chlorophyll and zinc oxide and copper oxide nanoparticles.
关键词： chlorophyll and nanoparticles,molecular interaction,ultraviolet-visible spectroscopy,laser-induced fluorescence spectroscopy,Attenuated total reflectance–Fourier transform infrared spectroscopy,steady state and time resolved fluorescence,X-ray diffraction
更新于2025-09-19 17:15:36
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Structural and Optical Properties of Silicon Nanowire Arrays Fabricated by Metal Assisted Chemical Etching With Ammonium Fluoride
摘要： Here we report on the metal assisted chemical etching method of silicon nanowires (SiNWs) manufacturing, where the commonly used hydrofluoric acid (HF) has been successfully replaced with ammonium fluoride (NH4F). The mechanism of the etching process and the effect of the pH values of H2O2: NH4F solutions on the structural and optical properties of nanowires were studied in detail. By an impedance and Mott-Schottky measurements it was shown that silver-assisted chemical etching of silicon can be attributed to a facilitated charge carriers transport through Si/SiOx/Ag interface. It was shown that the shape of nanowires changes from pyramidal to vertical with pH decreasing. Also it was established that the length of SiNW arrays non-linearly depends on the pH for the etching time of 10 min. A strong decrease of the total reflectance to 5–10% was shown for all the studied samples at the wavelength <800 nm, in comparison with crystalline silicon substrate (c-Si). At the same time, the intensities of the interband photoluminescence and the Raman scattering of SiNWs are increased strongly in compare to c-Si value, and also they were depended on both the length and the shape of SiNW: the biggest values were for the long pyramidal nanowires. That can be explained by a strong light scattering and partial light localization in SiNWs. Hereby, arrays of SiNWs, obtained by using weakly toxic ammonium fluoride, have great potential for usage in photovoltaics, photonics, and sensorics.
关键词： silicon nanowires,Raman scattering,impedance,photoluminescence,total reflectance
更新于2025-09-19 17:15:36
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Evaluation of Informative Bands Used in Different PLS Regressions for Estimating Leaf Biochemical Contents from Hyperspectral Reflectance
摘要： Partial least squares (PLS) regression models are widely applied in spectroscopy to estimate biochemical components through hyperspectral reflected information. To build PLS regression models based on informative spectral bands, rather than strongly collinear bands contained in the full spectrum, is essential for upholding the performance of models. Yet no consensus has ever been reached on how to select informative bands, even though many techniques have been proposed for estimating plant properties using the vast array of hyperspectral reflectance. In this study, we designed a series of virtual experiments by introducing a dummy variable (Cd) with convertible specific absorption coefficients (SAC) into the well-accepted leaf reflectance PROSPECT-4 model for evaluating popularly adopted informative bands selection techniques, including stepwise-PLS, genetic algorithms PLS (GA-PLS) and PLS with uninformative variable elimination (UVE-PLS). Such virtual experiments have clearly defined responsible wavelength regions related to the dummy input variable, providing objective criteria for model evaluation. Results indicated that although all three techniques examined may estimate leaf biochemical contents efficiently, in most cases the selected bands, unfortunately, did not exactly match known absorption features, casting doubts on their general applicability. The GA-PLS approach was comparatively more efficient at accurately locating the informative bands (with physical and biochemical mechanisms) for estimating leaf biochemical properties and is, therefore, recommended for further applications. Through this study, we have provided objective evaluations of the potential of PLS regressions, which should help to understand the pros and cons of PLS regression models for estimating vegetation biochemical parameters.
关键词： band selection,mechanism,PLSR,hyperspectral reflectance,PROSPECT
更新于2025-09-19 17:15:36
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Joint retrieval of surface reflectance and aerosol properties with continuous variation of the state variables in the solution space – Part 1: theoretical concept
摘要： This paper presents a new algorithm for the joint retrieval of surface reflectance and aerosol properties with continuous variations of the state variables in the solution space. This algorithm, named CISAR (Combined Inversion of Surface and AeRosol), relies on a simple atmospheric vertical structure composed of two layers and an underlying surface. Surface anisotropic reflectance effects are taken into account and radiatively coupled with atmospheric scattering. For this purpose, a fast radiative transfer model has been explicitly developed, which includes acceleration techniques to solve the radiative transfer equation and to calculate the Jacobians. The inversion is performed within an optimal estimation framework including prior information on the state variable magnitude and regularisation constraints on their spectral and temporal variability. In each processed wavelength, the algorithm retrieves the parameters of the surface reflectance model, the aerosol total column optical thickness and single-scattering properties. The CISAR algorithm functioning is illustrated with a series of simple experiments.
关键词： continuous variation,radiative transfer model,joint retrieval,aerosol properties,surface reflectance,CISAR algorithm,solution space,optimal estimation
更新于2025-09-19 17:15:36
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[Methods in Molecular Biology] Phototropism Volume 1924 (Methods and Protocols) || Determination of Phototropism by UV-B Radiation
摘要： UV-B phototropism in etiolated Arabidopsis seedlings has only been shown recently and needs further exploration. Here we elaborate on how to generate a customized setup with a unilateral UV-B light source, the required plant materials, different growth substrates, and a framework for data analysis.
关键词： Medium reflectance,Arabidopsis seedlings,Medium fluorescence,Growth substrates,Phototropism,UV-B,UV-B light sources
更新于2025-09-19 17:15:36
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Detection of pepper fusarium disease using machine learning algorithms based on spectral reflectance
摘要： The development of computerized automated diagnostic systems ensures more effective health screening in plants. In this way, the damage caused by diseases can be reduced by early detection. Light reflections from plant leaves are known to carry information about plant health. In the study, healthy and fusarium diseased peppers (capsicum annuum) was detected from the reflections obtained from the pepper leaves with the aid of spectroradiometer. Reflections were taken from four groups of pepper leaves (healthy, fusarium-diseased, mycorrhizal fungus, fusarium-diseased and mycorrhizal fungus) grown in a closed environment at wavelengths between 350 nm and 2500 nm. Pepper disease detection takes place in two stages. In the first step, the feature vector is obtained. In the second step, the feature vectors of the input data are classified. The feature vector consist of the coefficients of wavelet decomposition and the statistical values of these coefficients. Artificial Neural Networks (ANN), Naive Bayes (NB) and K-nearest Neighbor (KNN) were used for classification. In detection the health case of pepper, the average success rates of different classification algorithms for the first two groups (diseased and healthy peppers) were calculated as 100% for KNN, 97.5% for ANN and 90% for NB. Likewise, these rates for the classification of all groups were calculated as 100% for KNN, 88.125% for ANN and 82% for NB. Overall, the results have shown that leaf reflections can be successfully used in disease detection.
关键词： Wavelet,Spectral reflectance,Machine learning algorithms,Pepper disease detection,Classification
更新于2025-09-19 17:15:36
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Towards an optical diagnostic system for otitis media using a combination of otoscopy and spectroscopy
摘要： An improved method, where conventional otoscope investigation of human suspicious otitis media is combined with diffuse reflectance spectroscopy and gas in scattering media absorption spectroscopy (GASMAS) is being developed. Otitis media is one of the most common infectious diseases in children, whose Eustachian tube connecting the middle ear with the nasal cavity is more horizontal than for adults, which leads to impaired fluid drainage. At present, the use of an otoscope to visually observe possible changes in the tympanic membrane appearance is the main diagnostics method for otitis media. Inaccurate diagnosis related to similar symptoms, and the difficulty for small children to describe the condition experienced, frequently leads to over-prescription of antibiotics and alarming increase in bacterial resistance development. More accurate diagnostic methods are highly desirable. Diffuse reflectance spectroscopy is a non-invasive quantitative spectroscopic technique that enables to objectively quantify changes in the hemoglobin content of the tympanic membrane related to inflammation. If an infection is present, the ventilatory function of the Eustachian tube is frequently impaired and the middle-ear cavity will be filled with fluid. GASMAS, a non-invasive detection method, can non-invasively determine if gas is replaced by fluid in the middle-ear cavity.
关键词： otitis media,otoscope,diffuse reflectance spectroscopy,diagnostics,GASMAS,ear infection
更新于2025-09-19 17:15:36
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[IEEE 2019 IEEE 46th Photovoltaic Specialists Conference (PVSC) - Chicago, IL, USA (2019.6.16-2019.6.21)] 2019 IEEE 46th Photovoltaic Specialists Conference (PVSC) - Design of Thermophotovoltaic Cells for Optimal System Efficiency, Accounting for Photon Reuse from Front and Back Contacts
摘要： We develop a model showing how reflection from both the front and back contacts of thermophotovoltaic (TPV) cells affects the system efficiency. From this foundation we determine the design rules for front grids for TPV cells, and assess the consequences of those rules on cell design and system performance. We show that these design rules are different and more complex than the corresponding design rules for solar photovoltaic (PV) cells, and can lead to substantially different grid design and system performance than if the simpler solar PV design rules were used.
关键词： efficiency,grid reflectance,III-V,thermophotovoltaics,back surface reflectance
更新于2025-09-19 17:13:59
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Forensic soil analysis using laser-induced breakdown spectroscopy (LIBS) and Fourier transform infrared total attenuated reflectance spectroscopy (FTIR-ATR): Principles and case studies
摘要： Soils are crucial trace evidence that can establish or exclude the relationship between a suspect, victim, or an object at a particular scene, which could contribute to building a case. Laser-induced breakdown spectroscopy (LIBS) and Fourier transform infrared-attenuated total reflectance (FTIR-ATR) spectroscopy have been demonstrated to be effective techniques for soil characterization owing to its being rapid, non-destructive, and convenient analysis with little sample preparation requirements. Therefore, the principles of LIBS and FTIR-ATR techniques for soil forensic analysis in typical soil samples were investigated and their practical feasibility was tested by applying the techniques to forensic soil samples in two criminal cases. Principal component analysis (PCA) of a typical soil sample indicated that five typical soil types were clearly distinguished by LIBS and FTIR-ATR spectra. Variations in the soil elements (i.e., Si, Mg, Al, Ca, K, O, and N) and functional groups (i.e., O?H/N?H, C=C/C=O, Si?O, CO3 2?, Al?OH, and NH2) are crucial indicators for soil identification. The casework results demonstrated that both LIBS and FTIR-ATR show great potential for forensic soil analysis in future cases.
关键词： forensics,laser-induced breakdown spectroscopy,criminalistics,Soil identification,mid-infrared attenuated reflectance spectroscopy
更新于2025-09-19 17:13:59