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AIP Conference Proceedings [AIP Publishing LLC PROGRESS IN APPLIED MATHEMATICS IN SCIENCE AND ENGINEERING PROCEEDINGS - Bali, Indonesia (29 September–1 October 2015)] - Reflectance imaging by fiber bundle endoscope: Vertical reconstruction by multipositional illumination
摘要： Fiber bundles for imaging internal organs with minimum physical damage have been increasingly developed for both basic life sciences and clinical applications. Reflectance imaging is possible using fiber bundles for detecting the intrinsic optical contrast of blood vessels and tissue structure. The placement of an illumination source adjacent to imaging optics causes scattered light from deeper tissue layers to illuminate superficial tissues and results in a reflectance image. However, it does not have focal capacity and lacks depth resolution. In this study, we performed spatial analysis for the vertical reconstruction of in vivo tissues using a multipositional illumination scheme. The observed image corresponded to the “shadow” of a target object. When we manipulated the location of illumination, the shadow moved horizontally depending on the depth of the target. We used this horizontal displacement as a cue and successfully performed the vertical reconstruction of mouse brain blood vessels.
关键词： Fiber optics,Reflectance imaging,Endoscopy
更新于2025-09-23 15:21:21
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Rembrandt's <i>An Old Man in Military Costume</i> : Combining hyperspectral and MA-XRF imaging to understand how two paintings were painted on a single panel
摘要： Over the past several decades the painting An Old Man in Military Costume by Rembrandt Harmensz van Rijn (ca. 1630–31; J. Paul Getty Museum, 78.PB.246) has been the subject of a number of investigations carried out in order to better visualize a second painting beneath the surface figure. The underlying image – the head and shoulders of a man wearing a cloak – is oriented 180 degrees from the upper image and appears to be fairly complete. Scanning macro x-ray fluorescence (XRF) spectroscopy reveals the face is painted with lead white and a mercury-(likely vermilion), and the cloak is painted with a copper-containing pigment. Following the revelation and digital color reconstruction of the underlying figure, a number of questions still remained. Here, through the use of infrared reflectance imaging spectroscopy (i.e., hyperspectral imaging) and macro-XRF imaging spectroscopy, together with cross-sections taken from targeted areas, the sequence of painting in both compositions was explored. Of particular interest was the discovery of evidence of multiple attempts to situate the lower figure, and the subsequent application of a blocking-out layer over the lower figure before the artist rotated the panel and executed the upper figure. In addition, examination of the placement of the two images on the panel adds to our understanding of the subtle complexities of Rembrandt’s working process.
关键词： re-use of painting supports,Early Rembrandt,infrared reflectance imaging spectroscopy,X-ray fluorescence imaging spectroscopy
更新于2025-09-04 15:30:14