- 标题
- 摘要
- 关键词
- 实验方案
- 产品
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A robust and versatile platform for image scanning microscopy enabling super-resolution FLIM
摘要： Image scanning microscopy (ISM) can improve the effective spatial resolution of confocal microscopy to its theoretical limit. However, current implementations are not robust or versatile, and are incompatible with fluorescence lifetime imaging (FLIM). We describe an implementation of ISM based on a single-photon detector array that enables super-resolution FLIM and improves multicolor, live-cell and in-depth imaging, thereby paving the way for a massive transition from confocal microscopy to ISM.
关键词： super-resolution,SPAD array,fluorescence lifetime imaging,confocal microscopy,image scanning microscopy
更新于2025-09-23 15:23:52
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An 80 x 25 Pixel CMOS Single-Photon Sensor With Flexible On-Chip Time Gating of 40 Subarrays for Solid-State 3-D Range Imaging
摘要： A CMOS solid-state 3-D range imager that uses short (～110 ps) laser pulses and a novel flexible time gating scheme for a single-photon avalanche diode (SPAD) array is presented. The array is divided into 40 subarrays for which a narrow (<0.8 ns) time gating position can be set and scanned independently. The imager can be set to measure multiple regions of interest by means of the flexible time gating of the subarrays. The time gating for each of the subarrays is selected separately with an on-chip delay-locked loop (DLL) block that has 240 outputs and a delay grid of ～100 ps. The prototype has 80 × 25 pixels overall with 10 × 5 pixel subarrays. The fill factor of the sensor area is 32%. A 3-D range image is demonstrated at ～10 frames/s with centimeter-level precision in the case of passive targets within a range of ～4 m and a field of view of 18° × 28°, requiring an average active illumination power of only 0.1 mW.
关键词： time gating,3-D imager,SPAD array,single-photon avalanche diode (SPAD),time-of-flight (TOF)
更新于2025-09-19 17:15:36