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Formation of a keratin layer with silk fibroin-polyethylene glycol composite hydrogel fabricated by digital light processing 3D printing
摘要： Silk fibroin (SF)-incorporated 4-arm polyethylene glycol acrylate (PEG4A) was used as a bioink for digital light processing three-dimensional printing. A visible range of light was adopted for the process to assure the safety of the cells in the bioink. Fibroblast cells were embedded in the printed hydrogels, and the keratinocyte cells were cultured at the surfaces of the hydrogels. The proliferation of cells with the bioink materials, PEG4A and SF-PEG4A, and the toxicity of these materials, were verified by live/dead and alamarBlue assays. The primary human keratinocytes on the SF-PEG4A hydrogel scaffold generated a keratin layer by the tissue culture at the air-liquid interface, promoting maturation and stratification. SF-polyethylene glycol hydrogels showed higher cell proliferation and cell spreading, and the thicker keratin layer was formed with SF-PEG4A hydrogels compared with PEG4A hydrogels.
关键词： Digital light processing,Silk fibroin,3D printing,4-arm polyethylene glycol acrylate,Keratin
更新于2025-09-23 15:23:52
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Silk Fibroin-Based Films Enhance Rhodamine 6G Emission in the Solid State: A Chemical-Physical Analysis of their Interactions for the Design of Highly Emissive Biomaterials
摘要： Described herein is the preparation of dye-doped films employing silk fibroin (SF) as a biomaterial, capable of preserving the optical properties of the monomeric dye in the solid state, a critical requisite for optical and biolaser applications. A comprehensive physical–chemical characterization is reported for SF films doped with Rhodamine 6G, an ideal candidate for photonics and optoelectronics. Fourier transform infrared spectroscopy (FTIR), circular dichroism (CD) and X-Ray diffraction (XRD) provide information on SF secondary conformation in the presence of rhodamine. UV–vis absorption spectra and exciton CD inform on the structure of encapsulated rhodamine, while changes in dye photophysical properties illuminate the molecular mechanism of the involved host–guest interactions. SF host environment inhibits rhodamine dimer formation, indicating that SF is an optimum matrix to keep rhodamine essentially monomeric at concentrations as high as 7 mm in the film. The relevant optical properties of these films and the easiness of their preparation, make these systems optimal candidates for innovative photonic technologies.
关键词： FTIR,silk fibroin,dye-doped biomaterials,exciton coupled CD,fluorescence
更新于2025-09-23 15:23:52
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Contents: (Adv. Funct. Mater. 36/2018)
摘要： In article number 1800802, Hyunjoo J. Lee and co-workers present a calcium-modified silk fibroin film as a strong adhesive for next-generation epidermal electronics. The modified silk film exhibits viscoelastic properties and can mechanically interlock a sensor and the skin surface ~20 times stronger than commercial alternatives. Additional interesting characteristics, such as good biocompatibility, reusability, stretchability, and conductivity show promise for widespread application.
关键词： epidermal electronics,biocompatibility,conductivity,silk fibroin,reusability,stretchability
更新于2025-09-23 15:21:01
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Fluorescent DTPA-Silk Fibroin Nanoparticles Radiolabeled with <sup>111</sup> In: A Dual Tool for Biodistribution and Stability Studies
摘要： This work aims to provide an effective and novel dual tool for the biodistribution studies of biopolimeric nanoparticles by using modified silk fibroin nanoparticles as a model. This is an indispensable step in the evaluation of the applicability of biopolymeric nanoparticles as drug delivery systems. In this paper, we report a new facile method for radiolabeling silk fibroin nanoparticles conjugated to the chelating agent diethylenetriamine pentaacetic acid (DTPA) and tagged with fluorescein isothiocyanate (FITC). Nanoparticles were characterized by means of dynamic light scattering (DLS), scanning electron microscopy (SEM), infrared and fluorescence spectroscopy. The in vitro studies included the stability in biological media and the evaluation of the cytotoxicity of the nanoparticles in a cell culture. The in vivo study was focused in the scintigraphic study over 24 h conducted on New Zealand rabbits, after intra-articular injection of [111In]In-nanoparticles containing 8.03 ± 0.42 MBq. Biodistribution of the nanoparticles was assessed also ex vivo by fluorescence microscopy of post mortem biopsied organs. This radiolabeling method was reproducible and robust with high radiolabeling efficiency (~80 %) and high specific activity suitable for the in vivo studies. Radiolabeled nanoparticles, having hydrodynamic radius of 113.2 ± 2.3 nm, a polydispersity index (PdI) of 0.101 ± 0.015 and Z-potential of -30.1 ± 2.0 mV, showed and optimum retention in the articular space, without activity clearance up to 24 h post injection. Thus, an easy and robust radiolabeling method has been developed, and its applicability is demonstrated in vitro and in vivo studies, showing its value for future investigation of silk fibroin nanoparticles as versatile and stable (steady) local drug delivery systems for consideration as a therapeutic option, particularly in the treatment of joint disorders.
关键词： biodistribution,theragnostic,radiolabeling,mesenchymal stem cells culture,FITC,silk fibroin nanoparticles,Indium-111,stability
更新于2025-09-23 15:19:57
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Photoluminescent functionalized carbon quantum dots loaded electroactive Silk fibroin/PLA nanofibrous bioactive scaffolds for cardiac tissue engineering and nursing care application
摘要： Tissue engineering and stem cell rehabilitation are the hopeful aspects that are being investigated for the management of Myocardial Infarction (MI); cardiac patches have been used to start myocardial rejuvenation. In this study, we engineered p-phenylenediamine surface functionalized (modif-CQD) into the Silk fibroin/PLA (SF/PLA) nanofibrous bioactive scaffolds with improved physico-chemical abilities, mechanical and cytocompatibility to cardiomyocytes. The micrograph results visualized the morphological improved spherical modif-CQD have been equivalently spread throughout the SF/PLA bioactive cardiac scaffolds. The fabricated CQD@SF/PLA nanofibrous bioactive scaffolds were highly porous with fully consistent pores; effectively improved young modulus and swelling asset for the suitability and effective implantation efficacy. The scaffolds were prepared with rat cardiomyocytes and cultured for up to 7 days, without electrical incentive. After 7 days of culture, the scaffold pores all over the construct volume were overflowing with cardiomyocytes. The metabolic activity and viability of the cardiomyocytes in CQD@SF/PLA scaffolds were significantly higher than cardiomyocytes in Silk fibroin /PLA scaffolds. The integration of CQD also influenced greatly and increases the expression of cardiac- marker genes. The results of the present investigations evidently recommended that well-organized cardiac nanofibrous scaffold with greater cardiac related mechanical abilities and biocompatibilities for cardiac tissue engineering and nursing care applications.
关键词： Scaffolds,PLA,Carbon Quantum Dots,Silk fibroin
更新于2025-09-16 10:30:52
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Silk fibroin nanoparticles for enhanced bio-macromolecule delivery to the retina
摘要： The aim of this study was to investigate intravitreal injection of silk fibroin nanoparticles (SFNs) encapsulating bio-macromolecules, achieving enhanced drug bioavailability and extended retention in retina. SFNs were prepared with regenerated silk fibroin (RSF) using desolvation method with fluorescein isothiocyanate labeled bovine serum albumin (FITC-BSA) as bio-macromolecular model drug encapsulated. In vitro physicochemical properties and in vitro drug release of FITC-BSA loaded SFNs (FITC-BSA-SFNs) were evaluated. Cytotoxicity, cellular uptake, and retention of FITC-BSA-SFNs were determined in human retinal pigment epithelial cell line (ARPE-19). In addition, in vivo distribution and safety of intravitreally administered FITC-BSA-SFNs were investigated in New Zealand white rabbits. The particle size of FITC-BSA-SFNs was 179.1±3.7 nm with polydispersity index (PDI) of 0.102±0.033 and the zeta potential was greater than -25 mV. FITC-BSA-SFNs exhibited excellent biocompatibility with no cytotoxicity observed within 24 h and 48 h in AREP-19 cells. Compared to FITC-BSA solution, FITC-BSA-SFNs showed enhanced cellular uptake and prolonged retention. Furthermore, FITC-BSA-SFNs achieved accumulated distribution and extended retention in retina in vivo following intravitreal injection compared to a single administration of free drug solution. Therefore, this bio-macromolecule delivery platform based on SFNs could have great potential in the treatment of posterior segment disorders.
关键词： bio-macromolecules,Silk fibroin nanoparticles,ocular drug delivery,posterior segment diseases,intravitreal injection
更新于2025-09-10 09:29:36
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Biomaterial-Based “Structured Opals” with Programmable Combination of Diffractive Optical Elements and Photonic Bandgap Effects
摘要： Naturally occurring iridescent systems produce brilliant color displays through multiscale, hierarchical assembly of structures that combine reflective, diffractive, diffusive, or absorbing domains. The fabrication of biopolymer-based, hierarchical 3D photonic crystals through the use of a topographical templating strategy that allows combined optical effects derived from the interplay of predesigned 2D and 3D geometries is reported here. This biomaterials-based approach generates 2D diffractive optics composed of 3D nanophotonic lattices that allow simultaneous control over the reflection (through the 3D photonic bandgap) and the transmission (through 2D diffractive structuring) of light with the additional utility of being constituted by a biocompatible, implantable, edible commodity textile material. The use of biopolymers allows additional degrees of freedom in photonic bandgap design through directed protein conformation modulation. Demonstrator structures are presented to illustrate the lattice multifunctionality, including tunable diffractive properties, increased angle of view of photonic crystals, color-mixing, and sensing applications.
关键词： 3D hierarchical photonic structures,silk fibroin,structural color,multifunctionality,diffusion/diffraction
更新于2025-09-09 09:28:46
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Fluorescent boron Schiff bases dyes for staining silk fibroin: Green synthesis, structural characterization, DFT, and photophysical properties
摘要： Five novel organoboron complexes were synthesized in just 15 min via microware irradiation, by one pot multicomponent reactions between diverse aryl aldehydes with benzoylhydrazide, or 4‐nitrobenzoylhidrazine and diphenyl boronic acid, in a 1:1:1 ratio in benzene. The products were characterized by 1H, 13C, 11B NMR, UV, IR, spectroscopy and high‐resolution mass spectrometry (HRMS). The molecular structure was also determined by single‐crystal X‐ray diffraction for two complexes, which showed the tetra‐coordination of the boron atoms giving rise to distorted tetrahedral molecular geometry with a strong intermolecular C‐H···π interactions. In spite of the low quantum yields exhibited by the series in solution, some complexes stained uniformly the silk fibroins emitting enough fluorescence to allow its characterization by confocal microscopy. Boron as chelate center of the five complexes resulted not to be toxic for B16F10 cells, these compounds are appropriate for their used in medical applications.
关键词： organoboron,silk fibroin,cytotoxicity,confocal microscopy,fluorescent
更新于2025-09-09 09:28:46