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Discrimination of wood species using laser-induced breakdown spectroscopy and near-infrared reflectance spectroscopy
摘要： A new method is proposed for the discrimination of wood species by combining near-infrared reflectance spectroscopy (NIRS) and laser-induced breakdown spectroscopy (LIBS) and using chemometrics for data analysis. The method was applied to the analysis of 42 samples from six different species: Amburana cearensis, Copaifera lucens, Phyllocarpus riedelii, Cariniana legalis, Bowdichia virgilioides, and Aspidosperma pyricollum. The spectra from both techniques were merged on a single data matrix and pretreated by standard normal variate (SNV) and Savitzky– Golay first derivative with smoothing. Principal component analysis was applied to the exploratory data analysis and showed a clear formation of sample groups according to the wood species only when the data from both analytical techniques and the data pretreatment were used. Sample discrimination using partial least squares discriminant analysis was proved possible, but with an average misclassification of about 10%. Sample grouping and discrimination were shown to be probably related to different concentrations of iron, copper, zinc, and/or sodium (affecting the LIBS spectra) and lignin, water, cellulose, and/or hemicellulose (affecting the NIRS spectra).
关键词： wood species discrimination,laser-induced breakdown spectroscopy,near-infrared reflectance spectroscopy,chemometrics
更新于2025-09-12 10:27:22
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Short-Term Ultraviolet (UV)-A Light-Emitting Diode (LED) Radiation Improves Biomass and Bioactive Compounds of Kale
摘要： The aim of this study was to determine the influence of two types of UV-A LEDs on the growth and accumulation of phytochemicals in kale (Brassica oleracea var. acephala). Fourteen-day-old kale seedlings were transferred to a growth chamber and cultivated for 3 weeks. The kale plants were subsequently subjected to two types of UV-A LEDs (370 and 385 nm) of 30 W/m2 for 5 days. Growth characteristics were all significantly increased in plants exposed to UV-A LEDs, especially at the 385 nm level, for which dry weight of shoots and roots were significantly increased by 2.22 and 2.5 times, respectively, at 5 days of treatment. Maximum quantum efficiency of photosystem II photochemistry (Fv/Fm ratio) began to decrease after 3 h of treatment compared to the control. The total phenolic content of plants exposed to the two types of UV-A LEDs increased by 25% at 370 nm and 42% at 385 nm at 5 days of treatment, and antioxidant capacity also increased. The two types of UV-A LEDs also induced increasing contents of caffeic acid, ferulic acid, and kaempferol. The reactive oxygen species (ROS) temporarily increased in plants exposed to the two types of UV-A LEDs after 3 h of treatment. Moreover, transcript levels of phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), and flavanone 3-hydroxylase (F3H) genes and PAL enzyme activity were higher in plants treated with UV-A LEDs. Our results suggested that short-term UV-A LEDs were effective in increasing growth and improving antioxidant phenolic compounds in kale, thereby representing a potentially effective strategy for enhancing the production of phytochemicals.
关键词： transcript level,reactive oxygen species,phenolic compound,UV-A LEDs,kale,antioxidant capacity
更新于2025-09-11 14:15:04
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[Advances in Ecological Research] Mechanisms underlying the relationship between biodiversity and ecosystem function Volume 61 || Terrestrial laser scanning reveals temporal changes in biodiversity mechanisms driving grassland productivity
摘要： Biodiversity often enhances ecosystem functioning likely due to multiple, often temporarily separated drivers. Yet, most studies are based on one or two snapshot measurements per year. We estimated productivity using bi-weekly estimates of high-resolution canopy height in 2 years with terrestrial laser scanning (TLS) in a grassland diversity experiment. We measured how different facets of plant diversity (functional dispersion [FDis], functional identity [PCA species scores], and species richness [SR]) predict aboveground biomass over time. We found strong intra- and inter-annual variability in the relative importance of different mechanisms underlying the diversity effects on mean canopy height, i.e., resource partitioning (via FDis) and identity effects (via species scores), respectively. TLS is a promising tool to quantify community development non-destructively and to unravel the temporal dynamics of biodiversity-ecosystem functioning mechanisms. Our results show that harvesting at estimated peak biomass—as done in most grassland experiments—may miss important variation in underlying mechanisms driving cumulative biomass production.
关键词： functional diversity,functional identity,ecosystem functioning,Biodiversity,species richness,grassland,terrestrial laser scanning
更新于2025-09-11 14:15:04
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Smart Plasmonic Nanozyme Enhances Combined Chemo-Photothermal Cancer Therapy and Reveals Tryptophan Metabolic Apoptotic Pathway
摘要： The tumor microenvironment regulation is considered as an intelligent strategy for cancer therapeutics, but the related metabolic pathways of cell apoptosis still remains a great challenge. Herein, by applying multifunctional carbon dot-decorated Ag/Au bimetallic nanoshells (CDs-Ag/Au NSs, CAANSs) nanoprobes as smart plasmonic nanozymes for combined chemo-photothermal cancer therapy, we achieved a high efficiency in cancer cell therapy and revealed a tryptophan metabolic apoptotic pathway. In addition to high photothermal conversion efficiency, the CAANSs can act as a smart nanozyme to catalyze intracellular H2O2 to the cytotoxic reactive oxygen species (ROS) of superoxide anion (·O2-) in response to mild acidic cancerous cell microenvironment to damage cellular DNA. More importantly, the tryptophan metabolic pathway during the combined chemo-photothermal therapy has been revealed that the tryptophan participates in oxidative stress process, which can be decomposed to produce H2O2 and further formed into superoxide anion to kill cells under the catalytic nanomedicine process. The current work provides an effective platform for cancer therapeutics and is promising for cancer-related molecular biology studies.
关键词： Tryptophan Metabolic Apoptotic Pathway,Smart Plasmonic Nanozyme,Tumor Microenvironment,Chemo-Photothermal Cancer Therapy,Reactive Oxygen Species
更新于2025-09-11 14:15:04
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Spatial Heterogeneity of Cadmium Effects on Salvia sclarea Leaves Revealed by Chlorophyll Fluorescence Imaging Analysis and Laser Ablation Inductively Coupled Plasma Mass Spectrometry
摘要： In this study, for a first time (according to our knowledge), we couple the methodologies of chlorophyll fluorescence imaging analysis (CF-IA) and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS), in order to investigate the effects of cadmium (Cd) accumulation on photosystem II (PSII) photochemistry. We used as plant material Salvia sclarea that grew hydroponically with or without (control) 100 μM Cd for five days. The spatial heterogeneity of a decreased effective quantum yield of electron transport (ΦPSII) that was observed after exposure to Cd was linked to the spatial pattern of high Cd accumulation. However, the high increase of non-photochemical quenching (NPQ), at the leaf part with the high Cd accumulation, resulted in the decrease of the quantum yield of non-regulated energy loss (ΦNO) even more than that of control leaves. Thus, S. sclarea leaves exposed to 100 μM Cd exhibited lower reactive oxygen species (ROS) production as singlet oxygen (1O2). In addition, the increased photoprotective heat dissipation (NPQ) in the whole leaf under Cd exposure was sufficient enough to retain the same fraction of open reaction centers (qp) with control leaves. Our results demonstrated that CF-IA and LA-ICP-MS could be successfully combined to monitor heavy metal effects and plant tolerance mechanisms.
关键词： effective quantum yield (ΦPSII),non-photochemical quenching (NPQ),photoprotective mechanism,photochemical quenching (qp),photosynthetic heterogeneity,phytoremediation,reactive oxygen species (ROS),bioimaging,singlet oxygen (1O2),clary sage
更新于2025-09-11 14:15:04
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Mode-locked Tm-doped fiber laser with large modulation depth ReS <sub/>1.02</sub> Se <sub/>0.98</sub> nanosheet saturable absorber
摘要： Microcystis blooms and their associated microcystins pose a significant health risk to humans. Microcystis normally occurs as colonies in eutrophic water bodies, and its physiological tolerance to algaecides is dissimilar to that of unicellular forms. However, the differences of physiological response to algaecides between unicellular and colonial Microcystis have been poorly explored. The current study investigated the effects of hexane extract of Acorus calamus rhizome (HEACR) on the physiological and photosynthetic mechanisms of unicellular and colonial M. aeruginosa in the laboratory. We analyzed the cell density, reactive oxygen species (ROS) level, malonaldehyde (MDA) content, photosynthetic pigments, capsular polysaccharide (CPS), and photosystem (PS II) parameters of the two morphological forms of Microcystis. Our results show that HEACR suppresses the growth of both unicellular and colonial M. aeruginosa, increases the intracellular ROS level and cause lipid peroxidation, as well as exerting a detrimental effect on chlorophyll a (chl a) content and photosynthetic efficiency. Almost 100% inhibition was observed for unicellular and colonial M. aeruginosa after 3 d exposure to 50 and 100 mg L?1 HEACR, respectively. The ROS level increase, MDA accumulation, the chl a decrease and carotenoid increase in unicellular M. aeruginosa were all more obvious than that in colonial cells. The fall in photosynthetic efficiency of unicellular M. aeruginosa were also more significant than that of colonial cells. After 3d exposure, the maximum quantum yield of PS II photochemistry (Fv/Fm), effective quantum yield of PS II photochemistry (Fv’/Fm’) and effective quantum yield of photochemical energy conversion in PS II (YII) of unicellular M. aeruginosa was almost totally inhibited by 20 mg L?1 HEACR, while the Fv/Fm, Fv’/Fm’ and YII of colonial M. aeruginosa decreased by 43%, 26% and 66% for 100 mg L?1 of HEACR, respectively. Comparing the two morphological forms of Microcystis, colonies show a greater increase in CPS level to more effectively resist the stress of HEACR and to mitigate ROS generation thereby better defending against oxidative damage. Furthermore, colonial M. aeruginosa shows better photoprotection ability than the unicellular form when exposed to HEACR. The colonies also sustain their maximum electron transport rate, increase their tolerance to strong light, and maintain a higher ability to disperse excess energy. These results demonstrated that HEACR can significantly interfere with the growth and physiological processes of both unicellular and colonial M. aeruginosa, but that colonial M. aeruginosa has a greater ability to adjust physiological tolerance to resist the stresses of HEACR.
关键词： Reactive oxygen species,Acorus calamusrhizome extract,Microcystis aeruginosa,Physiological tolerance,Capsular polysaccharide,Chlorophyll fluorescence parameters
更新于2025-09-11 14:15:04
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Effect of light-emitting diodes (LEDs) on snow crab catch rates in the Barents Sea pot fishery
摘要： Snow crab (Chionoecetes opilio) has become an important species for the Norwegian seafood industry since its first commercial harvest in 2012. However, periodically catch rates can be low, causing a financial strain on the fishery. Thus, improving the catch rate of existing pot designs has the potential to significantly improve the profitability of fishing enterprises. In this study, we investigated whether the addition of low-powered purple and white light-emitting diode (LED) fishing lights inside the pots could improve catch rates of snow crab in the Barents Sea. Results showed that pots with purple lights harvested a 12.8% higher catch per unit effort (CPUE; number of crab per pot) of legal-sized crab, which was significantly more than the control pots (p ? 0.035); pots with white lights did not catch significantly more crab (p > 0.05). Pots equipped with only light (no bait) caught very few crabs and were not considered a viable alternative. Although purple LEDs increased snow crab capture, the economic benefits of using underwater lights in pots remains unclear given the high capital investment required.
关键词： underwater fishing light,Barents Sea snow crab fishery,catchability,invasive species,LEDs
更新于2025-09-11 14:15:04
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Improving LiDAR-based tree species mapping in Central European mixed forests using multi-temporal digital aerial colour-infrared photographs
摘要： Digital colour-infrared (CIR) aerial photographs, which have been collected routinely in many parts of the world, are an invaluable data source for the monitoring and assessment of forest resources. Yet, the potential of these data for automated individual tree species mapping remains largely unexplored. One way to maximize the usefulness of digital CIR aerial photographs for individual tree species mapping is to integrate them with modern and complementary remote sensing technologies such as the light detection and ranging (LiDAR) system and 3D segmentation algorithms. In this study, we examined whether multi-temporal digital CIR orthophotos could be used to further increase the accuracy of airborne LiDAR-based individual tree species mapping for a temperate mixed forest in eastern Germany. Our results showed that the texture features captured by multi-temporal digital CIR orthophotos under different view-illumination conditions were species-specific. As a consequence, combining these texture features with LiDAR metrics significantly improved tree species mapping accuracy (overall accuracy: 77.4%, kappa: 0.68) compared to using LiDAR data alone (overall accuracy: 69.3%, kappa: 0.58). Among various texture features, the average gray level in the near-infrared band was found to contribute most to the classification. Our results suggest that the synergic use of multi-temporal digital aerial photographs and airborne LiDAR data has the potential to accurately classify individual tree species in Central European mixed forests.
关键词： Digital CIR orthophotos,Texture features,Multi-temporal,Tree species,Airborne LiDAR
更新于2025-09-11 14:15:04
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Kinetics and modeling of artificial sweeteners degradation in wastewater by the UV/persulfate process
摘要： The frequent detection of artificial sweeteners (ASs) in wastewater and surface water gives rise to concerns about their removal, Acesulfame (ACE) and sucralose (SUC) are two ASs that are difficult to remove. The ultraviolet/persulfate (UV/PS) advanced oxidation technology (AOT) is being considered as an effective process for the degradation of micropollutants in wastewater. However, the study of the degradation of ASs in real wastewater by the UV/PS is minimal. This study investigated the kinetics and modeling of ACE and SUC degradation in wastewater by the UV/PS process. Both ACE and SUC could be degraded effectively using this process. The degradation of ACE was mainly attributed to UV photolysis (51%), HO· (26%) and SO4·- (16%), while that of SUC was mainly attributed to HO· (68%) and SO4·- (27%). The second-order rate constants of ASs with SO4·- were significantly lower than that with HO·. Three major transformation products (TPs) of ACE and four major TPs of SUC were identified. Additionally, the effects and mechanisms of the water matrices, such as HCO3-, Cl-, NO3- and natural organic matter (NOM), on ASs degradation were investigated through response surface methodology (RSM). NOM and Cl- significantly inhibited the degradation of ACE in the UV/PS system, whereas NOM and HCO3- played a main inhibition role on the degradation of SUC. A water matrices parameter model for predicting ASs degradation in real wastewater was established by RSM for the first time, and the removal of ACE and SUC was well predicted by the model.
关键词： reactive species,UV/persulfate,real wastewater,predicting model based on water matrices,artificial sweeteners
更新于2025-09-11 14:15:04
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Polyacrylamide-Based Biocompatible Nanoplatform Enhances the Tumor Uptake, PET/fluorescence Imaging and Anticancer Activity of a Chlorophyll Analog
摘要： In this report we demonstrate the outstanding advantages of multifunctional nanoplatforms for cancer-imaging and therapy. The non-toxic polyacrylamide (PAA) nanoparticles (size:18-25 nm) formulation drastically changed the pharmacokinetic profile of the 124I- labeled chlorophyll-a derivative (formulated in 10% ethanol in PBS) with a remarkable enhancement in tumor uptake, and significantly reduced uptake in spleen and liver. Among the various nanoformulations investigated, the 124I- labeled photosensitizer (dose: 0.6142 MBq), and the cyanine dye-nanoparticles (CD-NP) conjugate (dose 0.3 μmol/kg) in combination showed great potential for tumor imaging (PET/NIR fluorescence) in BALB/c mice bearing Colon26 tumors. Compared to free non-labeled photosensitizer, the corresponding PAA nanoformulation under similar treatment parameters showed a remarkable enhancement in long-term tumor cure by PDT (photodynamic therapy) and provides an opportunity to develop a single nanoplatform for tumor-imaging (PET/fluorescence) and phototherapy, a practical “See and Treat” approach.
关键词： Positron Imaging Tomography,Nanoparticles,photodynamic therapy,Reactive Oxygen species
更新于2025-09-10 09:29:36