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Utility of surface plasmon resonance response of silver nanoparticles for assay of Teicoplanin in human plasma using spectrofluorimetric technique
摘要： Teicoplanin (TEIC) is a glycopeptide antimicrobial medication for management of several bacterial infectious diseases caused by gram-positive bacteria including methicillin-resistant Staphylococcus aureus and Enterococcus faecalis. Novel, very simple, fast and cost-effective two spectrofluorimetric methods were developed for the ultra-trace determination of TEIC in pharmaceutical vials and human plasma. The investigated methods based on measuring the fluorescence of TEIC in methanol (method A) and enhancing its fluorescence by 10 folds using silver nanoparticles (AgNPs) without any solvent extraction (method B). The fluorescence of TEIC was investigated at 385nm (excitation at 335 nm) with calibration ranged from 1 to 25 ng mL?1 and from 0.6 to 30 ng mL?1 with Limit of detection (LOD) of 280 and 160 pg mL?1 for method A and B respectively. The established methods were optimized, validated and bio-analytically validated via ICH and US-FDA guidelines. The performed methods were used to determine TEIC in human plasma with high percentage recovery of 98.8 ± 1.75. Further, the proposed methods were applied to study the stability of TEIC after exposure to various degradation stress conditions and kinetic degradations.
关键词： Spectrofluorimetry,Teicoplanin,Silver nanoparticles,Metal-enhanced fluorescence,Human plasma analysis
更新于2025-09-23 15:23:52
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Development and Validation of Spectrophotometric and Spectrofluorimetric Methods for the Determination of Cyclobenzaprine HCl
摘要： Five simple and sensitive methods were developed for the determination of cyclobenzapirine hcl (CB) in presence of its degradation product anthraquinone (AQ). Method A dual wavelength spectrophotometry (DW); where two wavelengths were selected for the drug 283 and 306 nm in such a way that the difference in absorbance was zero for its degradation. Method B ratio difference spectrophotometry (RD) was depended on measuring the ratio difference between 290 and 305 nm. Method C was depended on measuring the peak amplitude of the first derivative of the ratio spectra (1DD) at 282 and 306 nm. Method D Isoabsorptive Point (ISO) at 280 nm Coupled with Second Derivative (2D). Method E depending on spectrofluorimetric determination of cyclobenzapirine HCl through quenching of uranyl acetate with ?exi 228 nm and ?em at 458 nm. Linearties were obtained in concentration range 5 μg/ml – 30 μg/ml in case of methods A, B, C and D, while in case of methods E linearity was obtained in concentration range of 1 μg/ml –10 μg/ml. The five methods were found to be specific for CB in presence of different concentration % of its degradation product. The five proposed methods were successfully applied for the determination of CB in Multirelax tablets. Statistical comparison between the results obtained by the proposed methods and that obtained by the official one for the determination of the drug was done, founding that there were no significant differences between them.
关键词： (1DD) Derivative ratio,Dual wavelength (DW),Isoabsorptive point (ISO),Spectrofluorimetry,Ratio difference (RD),Uranyl acetate,Cyclobenzapirine HCl (CB),Anthraquinone (AQ)
更新于2025-09-23 15:21:21
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A new spectrofluorimetric assay method for vandetanib in tablets, plasma and urine
摘要： Purpose: To develop a simple and sensitive spectrofluorimetric method for the determination of vandetanib (VDB) in tablets (containing 100 mg of the drug) and biological fluids (spiked human plasma and urine). Methods: The proposed method is based on examining the intrinsic fluorescence intensity of VDB in acetonitrile at 480 nm after excitation at 330 nm. Factors affecting fluorescence intensity of the cited drug (VDB), including the influence of pH, diluting solvent and time, were studied and optimized by one factor at a time approach. A calibration curve was constructed by plotting VDB fluorescence intensity at 480 nm versus VDB concentrations in ng mL-1. The method was validated according to the recommendations of International Conference on Harmonisation (ICH) for validation of the analytical procedures Results: The linearity range of the method was 20 – 600 ng mL-1, with limits of quantification (LOQ) and of detection (LOD) of 30.45 and 10.05 ng mL-1, respectively. The adopted method was applied successfully to the quantitation of VDB in pure powder form (100.90 ± 0.91 %), laboratory prepared tablets (97.86 ± 1.42 %), spiked human plasma (97.97 ± 2.36 %) and urine (97.59 ± 0.87 %). Comparison of the proposed method with that of liquid chromatography-tandem mass spectrometry showed that there was no significant difference (p < 0.05) between the two methods in terms of accuracy and precision. Conclusion: The proposed method is simple and highly sensitive and, consequently, can be applied to assay VDB in biological samples as well as in dosage form.
关键词： Human plasma,Dosage forms,Validation,Human urine,Assay,Spectrofluorimetry,Vandetanib
更新于2025-09-23 15:19:57
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Application of derivative emission fluorescence spectroscopy for determination of ibuprofen and phenylephrine simultaneously in tablets and biological fluids
摘要： Two sensitive, rapid, and accurate derivative emission spectrofluorimetric methods applying zero crossing techniques were developed for simultaneous determination of binary mixtures of ibuprofen (IBU) and phenylephrine hydrochloride (PHE) in pure powder, synthetic mixture and combined tablets. The proposed methods were performed via measuring the intersected drug derivative amplitude of one drug at the zero crossing points for the other one and vice versa. The two methods rely on the measurement of the combined drugs native fluorescence after excitation at 270 nm in methanol directly, followed by differentiation using first (D1) and second derivative (D2) techniques. Applying the D1, IBU was measured quantitatively at 293.1 nm at zero crossing of PHE, on the other side; PHE was measured quantitatively at 300.7 nm at zero crossing of IBU. By the same way, applying the D2, the wavelengths selected were 303.5 nm for IBU and 312.9 nm for PHE. The concentration plots of derivative fluorescence intensity were rectilinear over the range of 0.5- 10 μg/mL and 0.025- 0.5 μg/mL for IBU and PHE, respectively. The results obtained with average % recoveries ± RSD are 99.73 ± 0.72 (IBU, D1), 99.49 ± 0.95 (PHE, D1), 99.79 ± 0.47 (IBU, D2), and 99.88 ± 0.34 (PHE, D2) were in good agreement with the comparison method. The proposed methods offer high sensitivity that enable direct analysis of IBU and PHE in spiked human plasma. The proposed methods were entirely validated in terms of ICH guidelines.
关键词： phenylephrine (PHE),ibuprofen (IBU),spiked plasma,derivative emission,Spectrofluorimetry,tablets
更新于2025-09-11 14:15:04
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Stability-indicating micellar enhanced spectro-fluorometric determination of Daclatasvir in its tablet and spiked human plasma
摘要： A fast, simple and sensitive micellar enhanced spectrofluorimetric method is performed for the determination of Daclatasvir dihydrochloride (DAC) in its pharmaceutical dosage form and in spiked human plasma. The fluorescence intensity (FI) was measured at 367 nm after excitation at 300 nm. In aqueous solution, the FI of DAC was greatly enhanced by more than 110% in the presence of sodium dodecyl sulphate (SDS). The detection method was linear over the range of 12.93 to 161.60 ng/mL, with a limit of detection of 1.75 ng/mL. The proposed method was successfully applied to the determination of DAC in its pharmaceutical dosage form and the mean % recovery of DAC in spiked human plasma was 95.42 + 2.52. The developed methodology was also extended to stress studies of DAC after exposure to different forced degradation conditions including acidic, alkaline, photolytic, thermal and oxidative environments.
关键词： Spectrofluorimetry,Daclatasvir dihydrochloride (DAC) detection,spiked human plasma
更新于2025-09-10 09:29:36
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Determination of Ondansetron by Spectrofluorimetry: Application to Forced Degradation Study, Pharmaceuticals and Human Plasma
摘要： The current manuscript describes a validated, responsive and rapid spectrofluorimetric method for quantifying ondansetron (OND) in authentic form, spiked human plasma and dosage forms. This is the first reported fluorescence study of Ondansetron in Triton X 100 system. Various variables affecting fluorescence response were studied precisely and optimised. The described method involved the fluorescence measurement in Triton X 100 system at λem/λex 354/317 nm. The calibration plot attained linearity over concentration range of 0.2 – 2 μg/mL. The developed method has been extensively applied to degradation studies of OND as per International Conference on Harmonisation (ICH) guidelines by exposing to oxidative, thermal, photo, acidic and alkaline conditions and also the degradation pathway has been proposed.
关键词： Pharmaceutical dosage forms,Stability studies,Spiked human plasma,Ondansetron,Spectrofluorimetry
更新于2025-09-09 09:28:46
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Spectrofluorimetric and Spectrophotometric Determination of Troxerutin in Pharmaceutical Preparations
摘要： Two new, sensitive and simple spectrofluorimetric and spectrophotometric methods have been developed for the determination of troxerutin in pharmaceutical preparations. Troxerutin inhibits the activity of hemoglobin on the catalyzed reaction of H2O2 with rhodamine B, which is a highly sensitive fluorogenic and chromogenic reagent used in many investigations. The reaction product was measured by spectrofluorimetry at 575 nm after excitation at 550 nm. The percentage inhibition was directly proportional to the concentration over the range 0.04-1.5 μg/mL for the spectrofluorimetric method. The relation between the percentage inhibition of the absorbance at 550 nm and the concentration is rectilinear over the range 0.2-30 μg/mL. This method was applied successfully to the determination of troxerutin in pharmaceutical dosage form.
关键词： Spectrophotometry,Troxerutin,Spectrofluorimetry
更新于2025-09-04 15:30:14