Mitochondriaa??targeted Plasmonic Spiky Nanorods Augments the Clearance of Senescent Cell in Vivo

摘要： Cellular senescence is stress-induced irreversible growth arrest, and is thought to impair tissue function. The clearance of senescent cells can delay the features of senescence. Here, we report the development of plasmonic core–shell spiky nanorods (CSNRs) surface-modified with an anti- beta-2-microglobulin (aB2MG) antibody and triphenylphosphonium (TPP), to target the mitochondria in senescent cells. The results demonstrated that Near-infrared (NIR) light irradiated aB2MG–TPP@CSNRs selectively caused mitochondrial damage and cell-intrinsic apoptosis of senescent cells with relatively low NIR light power, and the ability of CSNRs to activate and amplify the immune response in vitro and in vivo was discovered. The mechanism was revealed that photo-induced reactive oxygen species (ROS) generation resulted in the senescent cell apoptosis and meanwhile immune adjuvant effect by CSNRs accelerated the clearance of senescent cells in mice. This study opens the way for the use of precisely regulated plasma nanostructures for immune adjuvant and photo-induced apoptosis for age-related senescence.