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[IEEE 2018 40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) - Honolulu, HI (2018.7.18-2018.7.21)] 2018 40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) - Non-invasive method to monitor molecular changes in human stratum corneum during acute barrier disruption using reflectance NIR spectroscopy
摘要： Stratum corneum is the outer most part of skin for barrier function. Disorder in stratum corneum is related with many skin diseases including acne, atopic dermatitis and psoriasis. In developed countries, about 20% of the population has disorder in the barrier function of stratum corneum. Adhesive tape stripping is a method to disrupt skin barrier function in studying disorder in stratum corneum. In this study, we obtained NIR (Near-Infrared) spectrum of human skin after tape stripping. Changes in skin spectra after barrier disruption were investigated through principal component analysis (PCA) of spectrum. PCA analysis revealed that peaks for –NH stretching and –CH vibration mainly contributed to the spectral variation caused by barrier disruption. Furthermore, second derivative of spectrum revealed that acute barrier disruption contributes to spectral changes in the region related with secondary structure of protein, lipid and water associated with lipid in stratum corneum. We demonstrated that acute barrier disruption affected features in NIR spectrum. These spectral changes revealed that acute barrier disruption affected keratin protein and ceramide in human stratum corneum. These results suggest that NIR spectroscopy can be used to monitor changes in filamentous network and lamellar structure in stratum corneum. NIR spectroscopy can provide non-invasive method to investigate skin disease related with barrier disruption by monitoring disturbance in protein and lipid structure in stratum corneum.
关键词： protein,barrier disruption,Stratum corneum,tape stripping,water,NIR spectroscopy,lipid
更新于2025-11-14 15:14:40
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Development of laser speckle blood flowmeter for evaluating the physiological function of skin
摘要： Objective: We developed and demonstrated laser speckle ?owgraphy (LSFG) for two-dimensional (2D) skin blood ?ow (SBF) measurements to facilitate the noninvasive comparisons of SBF between individuals. Approach: By using morphing technology with a face mesh to compare SBF spatial distributions among individuals, we examined the practicability of SBF measurement with LSFG. Main results: (1) The uniformity of SBF measurement was demonstrated by examinations at different distances and angles for healthy subjects. (2) Mean blur rate (MBR)—a blood ?ow index of LSFG—exhibited signi?cant correlation with the thermal diffusion method (TDM)—an established blood ?ow measurement method—suggesting that MBR is an effective index of SBF. (3) Blowout time, the half-width duration/duration of one cardiac cycle, exhibited signi?cant negative correlation with age and positive correlation with stratum corneum hydration. Signi?cance: These results suggest that LSFG is useful for evaluating SBF-related skin properties, and it has signi?cant potential in medicine and cosmetology.
关键词： aging,stratum corneum hydration,skin blood ?ow,laser speckle ?owgraphy,morphing,strain-gauge plethysmography,thermal diffusion
更新于2025-09-23 15:19:57
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Characterization and validation of an in vivo confocal Raman spectroscopy led tri‐method approach in the evaluation of the lip barrier
摘要： Background/Aim: It was the aim to establish and validate in vivo confocal Raman spectroscopy for characterization of the lip barrier in conjunction with transepidermal water loss (TEWL) and skin capacitance assessments. For the first time in vivo, barrier-relevant components of the lip (derived, natural moisturizing factors (NMFs) and ceramides are described. Methods: In 32 healthy volunteers, a dental tongue fixation device was inserted to prevent both voluntary and involuntary lip moisturization during measurements. Seventeen individual parameters relating to water, ceramide, and NMF content were assessed via Raman spectroscopy. Additionally, corneometry and TEWL were measured. To give a guidance for the required volunteer group size of future lip barrier studies for all test parameters, coefficients of variation (CV) were calculated and plots showing the required sample size for a given percentage treatment effect. Results: Raman spectroscopy assessed parameters on the lower lip comprehensively characterized the state of the lip barrier. Parameter variability was sufficiently low to corroborate changes in most parameters using relatively small study populations. Conclusions: Lip skin is comparatively well hydrated. Biophysical measurement of the lip barrier function is a challenge, as unconscious licking of the lower lip has to be prevented. In vivo confocal Raman spectroscopy provides insightful parameters for the characterization of the lip barrier and sufficiently low inter-individual variability to assess relatively small parameter changes employing relatively few study subjects. Differences at the molecular level and at a high spatial resolution are detectable, and these insights might provide a breakthrough in the evaluation of lip barrier function and developing solutions for lip care.
关键词： lip skin measurements,closed chamber transepidermal water loss,lip stratum corneum,skin hydration capacitance,Raman spectroscopy
更新于2025-09-12 10:27:22