A water-soluble benzoxazole-based probe: real-time monitoring PPi via situ reaction by two-photon cells imaging

摘要： Pyrophosphate (PPi) played crucial roles in various fundamental physiological processes. Herein, a two-photon absorption (TPA) “On-Off-On” type benzoxazole-based fluorescence probe BN was designed and synthesized, which detected PPi through Cu2+ displacing method in situ system in aqueous medium. The on-off-on process of BN recognizing PPi was verified by mass spectra and theoretical calculations, which was successfully applied in TPA cells imaging.