- 标题
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- 关键词
- 实验方案
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Graphene quantum dots enhanced ToF-SIMS for single-cell imaging
摘要： Time-of-flight secondary ion mass spectrometry (ToF-SIMS) has shown promising applications in single-cell analysis owing to its high spatial resolution molecular imaging capability. One of the main drawbacks hindering progress in this field is the relatively low ionization efficiency for biological systems. The complex chemical micro-environment in single cells typically causes severe matrix effects, leading to significant signal suppression of biomolecules. In this work, we investigated the signal enhancement effect of graphene quantum dots (GE QDs) in ToF-SIMS analysis. A × 160 magnification of ToF-SIMS signal for amiodarone casted on glass slide was observed by adding amino-functionalized GE QDs (amino-GE QDs), which was significantly higher than adding previously reported signal enhancement materials and hydroxyl group-functionalized GE QDs (hydroxyl-GE QDs). A possible mechanism for GE QD-induced signal enhancement was proposed. Further, effects of amino-GE QDs and hydroxyl-GE QDs on amiodarone-treated breast cancer cells were compared. A significant signal improvement for lipids and amiodarone was achieved using both types of GE QDs, especially for amino-GE QDs. In addition, ToF-SIMS chemical mapping of single cells with better quality was obtained after signal enhancement. Our strategy for effective ToF-SIMS signal enhancement holds great potential for further investigation of drug metabolism pathways and the interactions between the cell and micro-environment.
关键词： Signal enhancement,Single-cell analysis,Graphene quantum dots,Time-of-flight secondary ion mass spectrometry
更新于2025-11-14 15:32:45
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Thermal atomic layer deposition of AlOxNy thin films for surface passivation of nano-textured flexible silicon
摘要： Aluminum oxynitride (AlOxNy) films with different nitrogen concentration are prepared by thermal atomic layer deposition (ALD) for flexible nano-textured silicon (NT-Si) surface passivation. The AlOxNy films are shown to exhibit a homogeneous nitrogen-doping profile and the presence of an adequate amount of hydrogen, which is investigated by Time-of-Flight Elastic Recoil Detection Analysis (ToF-ERDA). The effective minority carrier lifetimes are measured after the NT-Si surface passivation; the minimum surface recombination velocity (SRV) of 5 cm-s-1 is achieved with the AlOxNy film in comparison to the Al2O3 and AlN films (SRV of 7–9 cm-s-1). The better SRV with AlOxNy film is due to the collective effect of field-effect passivation by the presence of fixed negative charges, and chemical passivation by the presence of hydrogen within the film. The capacitance-voltage, and conductance measurements also are carried out using metal-oxide-semiconductor structure to determine the fixed negative charge density (Nf,ox), and defect density of states (Dit) in the AlOxNy films. The better surface passivation is attributed to unusually large Nf,ox of ~6.07 × 1012 cm-2, and minimal Dit of ~1.01 × 1011 cm-2-eV-1 owing to the saturation of Si dangling bonds by the hydrogen within the AlOxNy film matrix after the annealing step.
关键词： Surface passivation,Time-of-flight elastic recoil detection analysis (ToF-ERDA),Aluminum oxynitride,Thermal atomic layer deposition,Black flexible silicon
更新于2025-10-22 19:40:53
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[IEEE 2017 IEEE Nuclear Science Symposium and Medical Imaging Conference (NSS/MIC) - Atlanta, GA (2017.10.21-2017.10.28)] 2017 IEEE Nuclear Science Symposium and Medical Imaging Conference (NSS/MIC) - Axial Fourier Rebinning for Time-of-Flight PET
摘要： Fully 3D time-of-flight (TOF) PET scanners offer the potential of substantially improved image quality in clinical PET imaging. The main challenges of 3D TOF PET imaging are the data storage with either list-mode or binned formats, and the reconstruction time using iterative algorithms. Previously, we derived the Fourier rebinning and consistency equations (FORCEs), and showed 3D TOF data can be fully characterized by two consistency equations. In this work, we present an exact Fourier rebinning for 3D TOF data based on the axial consistency equation to dramatically reduce the data storage and the reconstruction time. Starting from pre-corrected 3D TOF data, the axial Fourier rebinning algorithm estimates a 2D TOF sinogram for each transverse slice without information loss. Since the 3D TOF data are axially truncated, we provide a solution to estimate the missing portion in the oblique TOF projection data. The proposed axial Fourier rebinning for TOF data (axFRT) can take advantage of all the 3D TOF data statistics, and the rebinned 2D TOF data can then be reconstructed using any algorithms for 2D or 2.5D TOF reconstructions. The axFRT algorithm allows the axial data sets being rebinned independently, and there are tens of thousands of such data sets which can naturally take advantage of the massively parallel processors to dramatically speedup the rebinning. We show numerical simulations to demonstrate that axFRT produces accurate and unbiased rebinned sinograms even for TOF PET with large axial acceptance angle. The axFRT will be particularly useful for 3D TOF PET with large axial field of view for PET imaging applications including dynamic, whole- or total-body imaging.
关键词： image reconstruction,positron emission tomography (PET),time-of-flight,Axial Fourier rebinning
更新于2025-09-23 15:23:52
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Compact Real-Time Inter-Frame Histogram Builder for 15Bits High-Speed ToF-Imagers based on Single-Photon Detection
摘要： Time-of-flight image sensors based on single-photon detection, i.e. SPADs, require some filtering of pixel readings. Accurate depth measurements are only possible if the jitter of the detector is mitigated. Moreover, the time stamp needs to be effectively separated from uncorrelated noise such as dark counts and background illumination. A powerful tool for this is building a histogram of a number of pixel readings. Future generation of ToF imagers are seeking to increase spatial and temporal resolution along with the dynamic range and frame rate. Under these circumstances, storing the complete histogram for every pixel becomes practically impossible. Considering that most of the information contained by the histogram represents noise, we propose a highly efficient method to store just the relevant data required for ToF computation. This method makes use of the shifted inter-frame histogram (SifH). It requires a memory as low as 128 times smaller than storing the complete histogram if the pixel values are coded on up to 15 bits. Moreover, a fixed 28 words memory is enough to process histograms containing up to 215 bins. In exchange, the overall frame rate only decreases to one half. The hardware implementation of this algorithm is presented. Its remarkable robustness for a low SNR of the ToF estimation is demonstrated by Matlab simulations and FPGA implementation using input data from a SPAD camera prototype.
关键词： shifted inter-frame histogram (SifH),single-photon avalanche-diode (SPAD),real-time time-of-flight (ToF) estimation,ToF image sensor
更新于2025-09-23 15:22:29
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Hand Gesture Recognition in Automotive Human–Machine Interaction Using Depth Cameras
摘要： In this review, we describe current Machine Learning approaches to hand gesture recognition with depth data from time-of-flight sensors. In particular, we summarise the achievements on a line of research at the Computational Neuroscience laboratory at the Ruhr West University of Applied Sciences. Relating our results to the work of others in this field, we confirm that Convolutional Neural Networks and Long Short-Term Memory yield most reliable results. We investigated several sensor data fusion techniques in a deep learning framework and performed user studies to evaluate our system in practice. During our course of research, we gathered and published our data in a novel benchmark dataset (REHAP), containing over a million unique three-dimensional hand posture samples.
关键词： time-of-flight sensors,hand gesture recognition,automotive,human–machine interaction,neural networks
更新于2025-09-23 15:22:29
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Background Light Rejection in SPAD-Based LiDAR Sensors by Adaptive Photon Coincidence Detection
摘要： Light detection and ranging (LiDAR) systems based on silicon single-photon avalanche diodes (SPAD) offer several advantages, like the fabrication of system-on-chips with a co-integrated detector and dedicated electronics, as well as low cost and high durability due to well-established CMOS technology. On the other hand, silicon-based detectors suffer from high background light in outdoor applications, like advanced driver assistance systems or autonomous driving, due to the limited wavelength range in the infrared spectrum. In this paper we present a novel method based on the adaptive adjustment of photon coincidence detection to suppress the background light and simultaneously improve the dynamic range. A major disadvantage of fixed parameter coincidence detection is the increased dynamic range of the resulting event rate, allowing good measurement performance only at a specific target reflectance. To overcome this limitation we have implemented adaptive photon coincidence detection. In this technique the parameters of the photon coincidence detection are adjusted to the actual measured background light intensity, giving a reduction of the event rate dynamic range and allowing the perception of high dynamic scenes. We present a 192 × 2 pixel CMOS SPAD-based LiDAR sensor utilizing this technique and accompanying outdoor measurements showing the capability of it. In this sensor adaptive photon coincidence detection improves the dynamic range of the measureable target reflectance by over 40 dB.
关键词： system-on-chip (SoC),single-photon avalanche diode (SPAD),CMOS,light detection and ranging (LiDAR),time-of-flight (TOF),background light rejection
更新于2025-09-23 15:22:29
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[IEEE 2018 IEEE International Conference on Imaging Systems and Techniques (IST) - Krakow (2018.10.16-2018.10.18)] 2018 IEEE International Conference on Imaging Systems and Techniques (IST) - Assessment of a Markerless Optical Tracking Technique for Computer-Assisted Hip Surgery
摘要： This article presents an evaluation of a prototype optical tracking system intended for use in computer-assisted orthopedic surgery. The device is based on a smartphone with a built-in video camera, and an external miniature time-of-flight camera. It is able to automatically recognize a surgical instrument based on its shape and appearance, and to determine its orientation relative to an array of light-reflective markers. The experiments were done in a measurement configuration and with the instruments that are specific to computer-assisted implantation of the acetabular component in total hip replacement surgery. The evaluation included the recognition rate of the instruments, and the angular accuracy of the system. The results show, that the accuracy is comparable to that obtained by conventional tracking devices in clinical settings, and partially meets the requirements specified in the literature for hip surgery.
关键词： optical tracking,surgical navigation,accuracy,computer assisted surgery,time of flight camera
更新于2025-09-23 15:22:29
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[IEEE 2018 International Conference on Indoor Positioning and Indoor Navigation (IPIN) - Nantes, France (2018.9.24-2018.9.27)] 2018 International Conference on Indoor Positioning and Indoor Navigation (IPIN) - Bluetooth-Based Indoor Positioning Through ToF and RSSI Data Fusion
摘要： After several decades of both market and scientific interest, indoor positioning is still a hot and not completely solved topic, fostered by the advancement of technology, pervasive market penetration of mobile devices and novel communication standards. In this work, we propose a two-step model-based indoor positioning algorithm based on Bluetooth Low-Energy, a pervasive and energy efficient standard protocol. In the first (i.e. ranging) step a Kalman Filter (KF) performs the fusion of both RSSI and Time-of-Flight measurement data. Thus, we demonstrate the benefit of not relying only on RSSI, comparing ranging performed with or without the help of ToF. In the second (i.e. positioning) step, the distance estimates from multiple anchors are combined into a quadratic cost function, which is minimized to determine the coordinates of the target node in a planar reference frame. The proposed solution is tailored to reduce the computational effort and target real-time execution on an embedded platform, demonstrating a limited loss of performance. The paper presents an experimental setup and discusses meaningful results, demonstrating a robust BLE-based indoor positioning solution for embedded systems.
关键词： Time-of-Flight (ToF),Bluetooth Low Energy,Indoor positioning,data fusion,Kalman filter
更新于2025-09-23 15:21:21
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[IEEE 2018 9th International Conference on Ultrawideband and Ultrashort Impulse Signals (UWBUSIS) - Odessa, Ukraine (2018.9.4-2018.9.7)] 2018 9th International Conference on Ultrawideband and Ultrashort Impulse Signals (UWBUSIS) - Criteria for Selecting Object Coordinates at Probing by the Impulse UWB GPR with the “1Tx + 4Rx” Antenna System
摘要： An impulse UWB GPR antenna system consisting of one transmitter and four receivers has been designed for rapid detection of objects on, and under, the ground surface. The measured two-way times-of-flight for the reflected probing signals (from the radiator to the object and back to the four receivers) are used to calculate the (x, y, z) coordinates of the reflecting object. For cases where several reflected times-of-flight (due e.g. to reflections from the vehicle or other noise) are recorded, criteria enabling selection of unique coordinates that correspond to the true target have been determined and verified.
关键词： detection of object,UWB radar,time-of-flight,antenna system
更新于2025-09-23 15:21:21
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Low temperature platinum chemical vapor deposition on functionalized self-assembled monolayers
摘要： The reaction pathways of Pt CVD using (COD)PtMe2 – xClx (x = 0, 1, 2) have been investigated on functionalized self-assembled monolayers (SAMs) as models for organic substrates. Residual gas analysis for (COD)PtMe2 and (COD)PtMeCl is consistent with the loss of methyl radicals as the initial step in deposition, while for (COD)PtCl2, the first step is the loss of a chlorine radical. It is further shown using x-ray photoelectron spectroscopy and time-of-flight secondary ion mass spectrometry that the deposition process leads to chemical damage of the SAM layer and little Pt deposition. Using this understanding, it is demonstrated that the Pt CVD rate can be controlled using a radical trap. In the presence of 1,4-cyclohexadiene, a well-known alkyl radical trap, Pt deposition was increased by 5× to 10×, creating a room-temperature effective Pt CVD process.
关键词： time-of-flight secondary ion mass spectrometry,x-ray photoelectron spectroscopy,Pt CVD,chemical vapor deposition,self-assembled monolayers,radical trap
更新于2025-09-23 15:21:01