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Graphene quantum dot-gold hybrid nanoparticles integrated aptasensor for ultra-sensitive detection of vitamin D3 towards point-of-care application
摘要： Vitamin D is a sunshine vitamin required by the body for various physiological activities. Deficiency of vitamin D (≤ 29 ng mL-1) can cause dental diseases, sarcopenia, osteoporosis, depression, type 2 diabetes, cancer, etc. Additionally, elevated levels of vitamin D (>150 ng mL-1) can result in numerous infirmities such as anorexia, irregular heartbeat, hypercalcemia, fatigue, etc. Hence, periodic detection can help maintain an appropriate level (≥ 30 ng mL-1) of vitamin D in blood serum. Conventional techniques used for the detection of vitamin D are expensive, time consuming, require skilled work force and a specialised laboratory. Herein, we report a portable electrochemical aptasensor for the detection of vitamin D3 using graphene quantum dot-gold (GQD-Au) hybrid nanoparticles. The developed aptasensor has a linear range of 1 nM – 500 nM, limit of detection (LOD) of 0.70 nM (0.28 ng mL-1), limit of quantification (LOQ) of 2.09 nM (0.84 ng mL-1), sensitivity of 0.90 Ω nM-1 mm-2 and a response time <1 minute. The sensor shows high specificity towards vitamin D3, a good stability, shelf life of over 35 days and nearly 98% recovery with serum samples. The developed sensor has been integrated with controlled electronics, thus establishing a portable prototype.
关键词： electrochemical,graphene quantum dot-gold hybrid,aptasensor,Vitamin D3,nanoparticles
更新于2025-09-23 15:21:01
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Evaluation of a Ultraviolet B Light Emitting Diode (LED) for Producing Vitamin D <sub/>3</sub> in Human Skin
摘要： Aim: A commercially available light emitting diode (LED) that transmitted narrow band ultraviolet B (UVB) radiation was evaluated for its efficacy and efficiency to produce vitamin D3 in human skin. Materials and Methods: Human skin samples were obtained from surgical procedures. The LED had peak emission wavelength of 295 nm. Skin samples were exposed to the UVB-LED for varying times and then were analyzed by high-pressure liquid chromatography (HPLC) to determine the vitamin D3 content. Results: There was a statistically significant time- and dose- dependent increase in the percent of 7-dehydrocholesterol that was converted to vitamin D3 in the skin type II samples; 1.3%±0.5, 2.3%±0.6 and 4.5%±1.67 after exposure to 0.75 (11.7 mJ/cm2), 1.5 (23.4 mJ/cm2) and 3 (46.8 mJ/cm2) minimal erythemal doses (MEDs), respectively. Conclusion: The UVB-LED was effective and efficient in generating vitamin D3 in human skin, in vitro. The amount of vitamin D3 production increased in a dose-dependent fashion with increased UVB energy. UVB-LEDs can be developed for devices that can efficiently produce vitamin D3 in human skin.
关键词： vitamin D3,human skin,ultraviolet B radiation,Previtamin D3,ultraviolet radiation light emitting diode (LED)
更新于2025-09-23 15:21:01