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The Influence of Ceramic Far-Infrared Ray (cFIR) Irradiation on Water Hydrogen Bonding and its Related Chemo-physical Properties
摘要： The property of water is highly related to the earth's environment and climate change. The fundamental dynamical process of water is include formation and breaking of hydrogen bonds. This dynamic process, so far, is still poorly understood. We investigated weakening of the hydrogen bonds of water after ceramic Far-Infrared Ray (cFIR) irradiation and the resulting effects on physical and chemical properties of water. In this study, the Fourier transform infrared spectroscopy (FT-IR) was used to explore hydrogen bonding change of cFIR-irradiated water; in addition, capillary viscometers, Gas Chromatographs (GC), Differential Scanning Calorimetry (DSC), contact angles, Franz cells, High-Performance Liquid Chromatography (HPLC), and capillary electrophoresis analysis were used to evaluate its physical characteristics, such as viscosity, volatility, temperatures of water crystallization, surface tension, diffusion, hydrogen peroxide dissociation, solubility of solid particles, and changes in pH of acetic acid. The cFIR treated water decreased in viscosity and surface tension (contact angles), but increased in the solubility of solid particles, hydrogen peroxide dissociation, temperatures of water crystallization, and acidity of acetic acid. The weakening of water hydrogen bonds caused by cFIR irradiation is correspondent with our previous medical-biological studies on cFIR.
关键词： Hydrogen bonds,Fourier transform infrared spectroscopy (FT-IR),Contact angle,Ceramic far infrared ray (cFIR),Irradiation water,Solubility,Volatility
更新于2025-09-23 15:23:52
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High-Performance Lignin-based Water-Soluble Macromolecular Photoinitiator for the Fabrication of Hybrid Hydrogel
摘要： Developing low-migration macromolecular photoinitiators (macro PIs) is significant to achieve the sufficient biosafety of photopolymers. In this study, for the first time lignin was introduced into photoinitiating systems. A novel lignin-based water-soluble macro PI (L-PEG-2959) was designed and synthesized by introducing water-soluble PEG chain and photoinitiating moiety into lignin backbone simultaneously. A series of characterizations were performed, including 1H NMR, FTIR, UV-Vis, GPC and TGA analysis, which indicated that L-PEG-2959 has excellent light absorption property within 200–400 nm, high initiating efficiency and superior water-solubility. Additionally, L-PEG-2959 could be compatible well with glycidyl methacrylate modified gelatin (Gel-GMA) and initiate the photopolymerization of Gel-GMA quickly under UV irradiation to form a chemical crosslinking hybrid hydrogel. Compared with the neat hydrogel made by Gel-GMA initiated with Irgacure2959, a commercial small molecular PI, the hybrid hydrogels presented controllable swelling property, increased mechanical strength, remarkably reduced precipitates and enhanced biosafety. The results indicated the great potential of lignin-based macro PIs in preparing biosafety photopolymers.
关键词： macromolecular photoinitiator,Lignin,hybrid hydrogel,low migration,water solubility
更新于2025-09-23 15:22:29
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Synthesis of Water Soluble CuGaS2/ZnS Quantum Dots for Ultrasensitive Fluorescent Detection of Alkaline Phosphatase Based on Inner Filter Effect
摘要： Developing monitoring technique for alkaline phosphatase (ALP) is crucial due to the important role it plays in living cells. Here, a kind of biocompatible glutathione-modified CuGaS2/ZnS quantum dots (GSH-CGS/ZnS QDs) was used as a fluorescent substance and then fabricated “turn-off” fluorescent biosensor for detection of ALP by help of inner filter effect (IFE). Firstly, we prepared CuGaS2/ZnS (CGS/ZnS) QDs using solvothermal method and explored the efficient ligand (GSH) exchanges strategy for transferring oil-soluble CGS/ZnS QDs to aqueous phase. More importantly, we also explored the potential biological applications of the nanohybrid QDs. The obtained GSH-CGS/ZnS QDs emitted strong yellow fluorescence with the maximum excitation (400 nm) and emission (601 nm). Then, GSH-CGS/ZnS QDs were mixed with p-nitrophenylphosphate (PNPP) and ALP. PNPP could be hydrolyzed to p-nitrophenol (PNP) by help of catalysis of ALP, and the excitation spectrum of the GSH-CGS/ZnS QDs overlapped well with the absorption spectrum of PNP, so the fluorescence of GSH-CGS/ZnS QDs was initially quenched via the so-called “IFE”. Finally, a novel “turn-off” biosensor for sensitive detection of ALP in the range of 0.05-10 U L -1(R2 = 0.98) with a detection limit of 0.01 U L-1 was successfully obtained. Results indicated that I-III-VI2 nanocrystals have great potential for their promising biomedical application.
关键词： Inner filter effect,Alkaline phosphatase,GSH-CuGaS2/ZnS QDs,Fluorescent detection,Water solubility and biocompatibility
更新于2025-09-23 15:19:57
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Synthesis of water soluble silicon phthacyanine, naphthalocyanine bearing pyridine groups and investigation of their DNA interaction, topoisomerase inhibition, cytotoxic effects and cell cycle arrest properties
摘要： In this work, axially [3,5-bis(3-pyridin-4-ylpropoxy)phenyl]methoxy groups substituted silicon (IV) phthalocyanine 2, napthalocyanine 3 and their water soluble derivatives 2a, 3a were synthesized and DNA interaction, topoisomerases inhibitory properties, cytotoxicity against five carcinoma cell lines of water soluble derivatives were examined. The calf thymus DNA (CT-DNA) binding properties of 2a and 3a were monitored using UV-Vis spectroscopy and agarose gel electrophoresis. The results showed that the compounds interacted to CT-DNA via intercalation with Kb values of 3.94 ± (0.15) ×106 and 1.08 ± (0.10) ×105 M-1. In DNA cleavage studies, the compounds cleaved to supercoiled pBR322 plasmid DNA significantly with irradiation as compared to without irradiation. The results of topoisomerases studies claimed that both compounds had inhibitory effects against topoisomerases. In the cytotoxicity studies, the CC50 values of the compounds were found in range of 2.15-52.97 μM against to five carcinoma cell lines. The SI values of 3a were found as 4.51, 1.43 and 3.66 against A549, BT20 and SK-MEL 128 cell lines. The cell cycle arrest was investigated against A549 cells by flow cytometry and obtained results indicated that both compounds induce cell cycle arrest at G0/G1 phase. All of obtained results demonstrated that 3a had lead anticancer agent toward lung, breast and melanoma due to its high selectivity to cancer cell lines.
关键词： Water solubility,Cytotoxicity,Topoisomerases.,DNA,Phthalocyanine,Cell cycle arrest
更新于2025-09-19 17:15:36
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A efficient light-controlled nitric oxide releaser in aqueous solution and its red fluorescence imaging in lysosome
摘要： In this work, a sensitive light-controlled turn on nitric oxide releaser, chromenylium-aniline-N-nitroso (CA-NO), was developed, which could release nitric oxide in pure water media and be applied for the red fluorescence imaging in lysosome. Upon the irradiation, fluorescence of the nitric oxide releaser enhanced quickly due to the light-controlled CA-NO being decomposed into a free radical nitric oxide and the fluorescent group CA, which destroyed of ICT structure. The mechanism was supported by the capture of nitric oxide by the commercial nitric oxide fluorescent probe DAN and the HRMS spectra peak (HRMS calcd for C30H34N3O4+: 526.2700, found: 526.2700.) belonging to CA. The fluorescence quantum yield (ΦF) of CA-NO changed from 0.54% to 3.24%. The nitric oxide releasing yield was calculated to be 91.3%, which proved the superiority of CA-NO. With the irradiation, the color of the solution immediately changed from pink to blue purple in visible light which realized the naked-eyed monitoring of nitric oxide releasing. Obvious fluorescence changes and high cell viability above 80% encouraged us to apply CA-NO into living cells and the CA-NO successfully released nitric oxide in living cells through under irradiation. Nitric oxide could easily diffuse into lysosomes and react with related proteins, which prompts nitric oxide to regulate lysosomal catabolism and autophagy. Therefore, the co-location experiment of CA-NO and Lyso Tracker Green in living cells was carried out and the co-localization coefficient was calculated to be 0.842, which implied that morpholine group promoted the nitric oxide releaser to be aggregate in lysosome successfully. This work achieved the fixed-point and quantitative release of nitric oxide, which was helpful to understand the roles of nitric oxide and provided a good basis for our studying on related interaction between protein and nitric oxide in lysosome and cells.
关键词： Light control,Water solubility,Lysosome localization,Red emission
更新于2025-09-16 10:30:52
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A novel approach to coat silica on quantum dots: Forcing decomposition of tetraethyl orthosilicate in toluene at high temperature
摘要： The coating of silica (SiO2) on quantum dots (QDs) has been widely studied, because SiO2 can protect QDs from the damages of moisture, radiation, and heat. Conventional SiO2 coating methods for QDs are usually performed in aqueous or emulsion solutions, which require the addition of water for the hydrolysis of SiO2 precursors and lead to the photoluminescence (PL) quenching of QDs. To address this issue, a novel SiO2 coating approach on single particle level was developed by the thermally forcing decomposition of tetraethyl orthosilicate in toluene. The CdSe/CdS/ZnS:Al@SiO2 nanoparticles (NPs) were prepared without decreasing the original PL quantum yield (QY), which exhibited much better photo and thermal stability in comparison with uncoated CdSe/CdS/ZnS:Al QDs. Furthermore, due to the natural formation of silanol groups on the SiO2 shell, CdSe/CdS/ZnS:Al@SiO2 NPs present not only good solubility but also excellent room temperature stability in phosphate buffer saline solution for several months.
关键词： SiO2,TEOS,stability,core/shell quantum dots,water solubility
更新于2025-09-11 14:15:04
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Paracetamol micro-structure analysis by optical mapping
摘要： Domain structure of paracetamol - popular antipyretic analgesic - was investigated by infrared (IR) spectroscopy using synchrotron radiation. Absorbance and retardance maps reveal molecular orientation inside the micro-domains of the paracetamol form II which has a better water solubility and compressibility compared to the commercially used forms I. The developed method of analysis representing orientation of optical slow(fast)-axis is compared with azimuthal orientation of the absorbance at several specific IR bands using vector maps. High brightness of synchrotron radiation and hyper-spectral mapping of structural domains in paracetamol clearly reveals the domain boundaries and can potentially be used to observe in situ intra-phase transformations of paracetamol forms-I, II, III and melting, which are important for making medical tablets and powders by an industrial process.
关键词： Paracetamol,Water solubility,Retardance,Orientational anisotropy,Absorbance
更新于2025-09-04 15:30:14