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Near-Infrared, Light-Triggered, On-Demand Anti-inflammatories and Antibiotics Release by Graphene Oxide/Elecrospun PCL Patch for Wound Healing
摘要： Very recently, significant attention has been focused on the adsorption and cell adhesion properties of graphene oxide (GO), because it is expected to allow high drug loading and controlled drug release, as well as the promotion of cell adhesion and proliferation. This is particularly interesting in the promotion of wound healing, where antibiotics and anti-inflammatories should be locally released for a prolonged time to allow fibroblast proliferation. Here, we designed an implantable patch consisting of poly(caprolactone) electrospun covered with GO, henceforth named GO–PCL, endowed with high ibuprofen (5.85 mg cm?2) loading, used as anti-inflammatory and antibiotic models respectively, and capable of responding to near infrared (NIR)-light stimuli in order to promptly release the payload on-demand beyond three days. Furthermore, we demonstrated the GO is able to promote fibroblast adhesion, a key characteristic to potentially provide wound healing in vivo.
关键词： polycaprolactone,plasma,wound healing,graphene oxide,vancomycin,on-demand drug release
更新于2025-09-19 17:13:59
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Randomized, Controlled Early Intervention of Dynamic Mode Fractional Ablative CO <sub/>2</sub> Laser on Acute Burn Injuries for Prevention of Pathological Scarring
摘要： This study challenges the currently accepted treatment parameters of waiting 1 year following burn injury to treat the resultant scarring. It assesses the impact of treating burn and trauma wounds at less than 3 months of injury on scar formation, providing a new temporal paradigm to patients in the prevention and minimization of scar formation. This randomized clinical trial included 19 patients with moderate‐to‐severe acute burn injuries less than 3 months from injury. The treatment areas underwent three fractional ablative CO2 laser treatments at 8‐week intervals. The primary outcomes were improvement in the Manchester Scar Scale (MSS) and photography. Secondary outcomes were optical coherence tomography (OCT), treatment time, immediate post‐treatment response, and histology. Upon blinded evaluation, 100% of treatment areas were correctly identified when compared with control. MSS scores before and after treatment were totaled with an averaged sum of 13.33 per scar at baseline to 8.39 after treatment, a 63% average improvement versus control (P < 0.001). Histology and OCT demonstrated a re‐organization of the underlying skin structure. The average treatment time was 18 minutes. Immediate post‐treatment responses were limited to mild‐to‐moderate erythema. Early intervention with ablative fractional laser on acute traumatic wounds resulted in significant decrease of scar formation compared with untreated areas on the same wounds, providing an impactful new time course to treat severe burn and trauma injuries.
关键词： scar prevention,fractional ablative laser,burn scars,scars,wound healing
更新于2025-09-19 17:13:59
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Blend biopolymeric nanofibrous scaffolds of cellulose acetate/?μ-polycaprolactone containing metallic nanoparticles prepared by laser ablation for wound disinfection applications
摘要： Laser ablation technique was utilized to synthesize versatile metallic nanoparticles including ZnO, Ag and CuO which were incorporated into the blend matrix of cellulose Acetate (CA) and ε-polycaprolactone (PCL) nano?brous scaffold. The compositional, microstructural and morphological behaviors for the obtained nano?bers were investigated using X-ray diffraction (XRD), Fourier Transformed Infrared, Transmission Electron Microscope (TEM) and Field Emission-Scanning Electron Microscope (FESEM). The cell viability and antibacterial activity were investigated against Staphylococcus aureus (S. aureus) and Escherichia coli (E-coli). TEM micrographs refer that while CuONPs were involved in the middle of CA/PCL ?brous scaffold with diameters around 160 nm. The morphological investigations indicated the scaffolds were con?gured in a non-oriented form with diameters 0.45–0.9 μm in the case of ZnONPs involved in blend matrix ?bers. The ratio of viable cells displays that compositions are biocompatible, while the antibacterial activity of both AgNPs and CuONPs showed an inhibition zone around 11.2.3 ± 2.2 mm and 9.4 ± 1.2 mm respectively. Bio-blend polymers matrices carrying nanoparticles could be tailored for a plethora of biomedical applications upon their compositions.
关键词： Laser ablation,CuO,Antibacterial,Ag nanoparticles,Wound healing,Blend polymer
更新于2025-09-19 17:13:59
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Development of Narrow Band LED Sources and Their Application to Dermatology
摘要： 半導体を用いた LED を光源とし，バンドパスフィルタを用いて超狭帯域光を得る技術を開発した．この光源をヒトの毛髪再生に応用し，有効な臨床結果を得ることができた．また超狭帯域 LED 照射が人の毛乳頭細胞を刺激し，mRNA の発現や蛋白生成が生じる事が分子生物学的に示された．更に皮膚深達度が高い超狭帯域赤色 LED 光照射が毛成長に有効であることがマウスの実験や，又培養ヒト毛乳頭細胞を用いた実験から分子メカニズムモデルが示された．逆に皮膚深達度が浅い超狭帯域緑色 LED 光照射は，創傷治癒に有効であった．このように，LED を光源とした超狭帯域光は，更に上述の研究を深化させるとともに，新たな光医学融合分野を発展させ新産業を開拓していくものと期待している．
关键词： Hair growth,Laser diode (LD),Human dermal papilla cells,Narrow-band LED (NB-LED),Light source,m-RNA,Wound healing,Band-pass filter
更新于2025-09-19 17:13:59
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Light-emitting diodes in dermatology: stimulation of wound healing
摘要： Low-level light therapy (LLLT), which is sometimes included in phototherapy, is an effective therapeutic strategy to improve wound healing and reduce pain, inflammation and swelling. Nowadays, new sources of light, such as light-emitting diodes (LEDs) with a broad range of wavelengths, are widely available. The biological effects promoted by LEDs are dependent on irradiation parameters, mainly wavelength and dose. This review article focuses on recent clinical trials using light-emitting diode low-level light therapy (LED-LLLT) for enhancing wound healing. In this article, we also cover the mechanisms of action of LLLT on cells and tissues and highlight the importance of defining optimum LLLT parameters for stimulation of wound healing.
关键词： phototherapy,light-emitting diode (LED),wound healing
更新于2025-09-19 17:13:59
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Fluorescence Lifetime Imaging Ophthalmoscopy of the Retinal Pigment Epithelium During Wound Healing After Laser Irradiation
摘要： Purpose: To investigate the change in fluorescence lifetime of retinal pigment epithelium (RPE) after laser irradiation by using an organ culture model. Methods: Porcine RPE-choroid-sclera explants were irradiated with selective retina treatment laser (wavelength: 527 nm, beam diameter: 200 lm, energy: 80–150 lJ). At 24 and 72 hours after irradiation, the mean fluorescence lifetime (sm) was measured with fluorescence lifetime imaging ophthalmoscopy (FLIO) (excitation wavelength: 473 nm, emission: short spectral channel: 498-560 nm, long spectral channel: 560–720 nm). For every laser spot, central damaged zone (zone 1: 120 3 120 lm), area including wound rim (280 3 280 lm except zone 1), and environmental zone (440 3 440 lm except zone 1 and 2) were analyzed. Peripheral zone at a distance from laser spots longer than 2000 lm was examined for comparison. Cell viability was evaluated with calcein-acetoxymethyl ester and morphology with fluorescence microscopy for filamentous-actin. Results: The RPE defect after selective retina treatment was mostly closed within 72 hours. FLIO clearly demarcated the irradiated region, with prolonged sm at the center of the defect decreasing with eccentricity. In short spectral channel, but not in long spectral channel, sm in the environmental zone after 72 hours was still significantly longer than in the peripheral zone. Conclusions: FLIO may clearly demarcate the RPE defect, demonstrate its closure, and, moreover, indicate the induced metabolic changes of surrounding cells during wound healing. Translational Relevance: This ex vivo study showed that FLIO may be used to evaluate the extent and quality of restoration of the damaged RPE and to detect its metabolic change in human fundus noninvasively.
关键词： selective retina therapy,retinal pigment epithelium,fluorescence lifetime,energy metabolism,wound healing
更新于2025-09-16 10:30:52
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ToF-SIMS and TIRF microscopy investigation on the effects of HEMA copolymer surface chemistry on spatial localization, surface intensity, and release of fluorescently labeled keratinocyte growth factor
摘要： The need for direct biomaterial-based delivery of growth factors to wound surfaces to aid in wound healing emphasizes the importance of interfacial interactions between the biomaterial and the wound surface. These interactions include the spatial localization of growth factor, the surface intensity of growth factor in contact with the wound, and the release profile of growth factor to the wound surface. The authors report the use of time-of-flight secondary ion mass spectrometry to determine the relationship between biomaterial surface chemistry and the spatial localization of growth factor. They have implemented a novel application of total internal reflectance fluorescence (TIRF) microscopy to measure the surface intensity and release of growth factor in contact with a glass substrate that has been used to model a wound surface. Detailed information regarding TIRF experiments has been included to aid in future studies regarding the biomaterial delivery to interfaces. The authors have evaluated the effects of (hydroxyethyl)methacrylate (HEMA) homopolymer, 5.89% methyl methacrylate/HEMA, and 5.89% methacrylic acid/HEMA surface chemistry on the spatial localization of AlexaFluor 488-labeled keratinocyte growth factor (AF488-KGF), AF488-KGF surface intensity at the copolymer surface, and release to a glass substrate. KGF is known to promote re-epithelialization in wound healing. The results show that the two copolymers allow for increased surface coverage, surface intensity, and release of AF488-KGF in comparison to the homopolymer. It is likely that differences in these three aspects could have a profound effect on the wound healing response.
关键词： ToF-SIMS,TIRF microscopy,HEMA copolymer,keratinocyte growth factor,wound healing
更新于2025-09-16 10:30:52
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FAILED LASER RETINOPEXY IN A PATIENT UNDERGOING PAZOPANIB THERAPY
摘要： To report a case of failed endolaser retinopexy in patient taking pazopanib and possible association with impaired retinal remodeling. Methods: Case report. Results: A 68-year-old man with a history of radiation-induced high-grade metastatic mediastinal leiomyosarcoma, on pazopanib 600 mg daily, presented with a superior retinal tear 2 months after initiating pazopanib. The patient was treated with pars plana vitrectomy and endolaser retinopexy without any immediate complications. One month postoperatively, the patient was found to have a new superior macula-sparing rhegmatogenous retinal detachment extending through the laser barricade nasally. A large proportion of the laser lesions in this area had notably remained chalk-white. The patient underwent pars plana vitrectomy with scleral buckling, and repeat endolaser retinopexy. Pazopanib therapy was stopped 1 week later in consultation with the oncology team and was reinitiated after the retina seemed stable under silicone oil for several weeks. The scars from the patient’s repeat endolaser retinopexy remained pale white at his clinic visit 2 months after the procedure, and at that time, his initial laser scars had only just begun to darken with pigment. Four and a half months after the initial vitrectomy and endolaser retinopexy, and three and a half months after his repeat vitrectomy and endolaser retinopexy, all the patient’s laser scars have developed increased pigment, and his retina remains completely flat under silicone oil tamponade. Conclusion: Pazopanib may have had an adverse effect on retinal wound healing after vitrectomy with endolaser retinopexy, leading to persistence of pale laser lesions many weeks after laser application. It is possible that this may have impaired the typical development of chorioretinal laser scar formation and led to subsequent retinal detachment.
关键词： pazopanib,retinal remodeling,scar formation,retinal detachment,retinopexy,retinal laser photocoagulation,wound healing
更新于2025-09-12 10:27:22
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Blinded, Randomized, Controlled Trial Evaluating the Effects of Light-Emitting Diode Photomodulation on Lower Extremity Wounds Left to Heal by Secondary Intention
摘要： BACKGROUND Light-emitting diode (LED) has been used for wound healing because of its stimulatory effects on fibroblast proliferation, matrix synthesis, angiogenesis, and downmodulation of inflammatory reactions. OBJECTIVE The aim of the authors’ study was to investigate the effects of red LED (wavelength 633 nm) photomodulation on lower extremity surgical defects left to heal by secondary intention. MATERIALS AND METHODS Fourteen subjects with surgical defects of the lower leg were irradiated with a 633 ± 3-nm light source for 20 minutes (105 mW/cm2, 126 J/cm2) at 4 weekly sessions. RESULTS The number of days required for wounds to heal was greater in the treatment group (63.2 ± 12.2 days) than in the control group (48.67 ± 11.1 days), although this difference was not statistically significant (p = .07). The percentage of the original wound remaining was not statistically different between treatment and control groups between Weeks 1 and 2 (p = .71) and Weeks 3 and 4 (p = .56). It was significant between Weeks 2 and 3 (p = .01). CONCLUSION This study revealed that red LED photomodulation at a wavelength of 633 nm did not result in clinical improvement in wound healing of surgical defects on the lower extremities.
关键词： lower extremity,secondary intention,photomodulation,wound healing,Light-emitting diode
更新于2025-09-12 10:27:22
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Sandwich-structure transferable free-form OLEDs for wearable and disposable skin wound photomedicine
摘要： Free-form optoelectronic devices can provide hyper-connectivity over space and time. However, most conformable optoelectronic devices can only be fabricated on flat polymeric materials using low-temperature processes, limiting their application and forms. This paper presents free-form optoelectronic devices that are not dependent on the shape or material. For medical applications, the transferable OLED (10 μm) is formed in a sandwich structure with an ultra-thin transferable barrier (4.8 μm). The results showed that the fabricated sandwich-structure transferable OLED (STOLED) exhibit the same high-efficiency performance on cylindrical-shaped materials and on materials such as textile and paper. Because the neutral axis is freely adjustable using the sandwich structure, the textile-based OLED achieved both folding reliability and washing reliability, as well as a long operating life (>150 h). When keratinocytes were irradiated with red STOLED light, cell proliferation and cell migration increased by 26 and 32%, respectively. In the skin equivalent model, the epidermis thickness was increased by 39%; additionally, in organ culture, not only was the skin area increased by 14%, but also, re-epithelialization was highly induced. Based on the results, the STOLED is expected to be applicable in various wearable and disposable photomedical devices.
关键词： Keratinocytes,Wearable photomedicine,Skin wound healing,Cell migration,Epidermis thickness,Re-epithelialization,Free-form optoelectronic devices,Sandwich-structure transferable OLED,Cell proliferation
更新于2025-09-12 10:27:22