- 标题
- 摘要
- 关键词
- 实验方案
- 产品
-
<b> Comparative study of Low-level laser therapy and microcurrent on the healing of skin burns in rats</b>
摘要： This study investigated and compared the effects of low-level laser therapy (LLLT) and microcurrent in the burn healing process in Wistar rats. We conducted a randomized controlled study with 30 rats divided into 3 groups (n = 10); control group (CG), laser group (LG) and microcurrent group (MG). After thermal damage, 10 applications of 660 nm diode laser were performed in GL and 10 applications of 60 Hz microcurrent (160 μA) in MG. The semi-quantitative histological analysis was done using scores (0–3), in sections stained by hematoxylin and eosin and Masson’s trichrome. The results indicated a significant improvement in the fibroblasts proliferation, collagen fibers deposition, neoangiogenesis, and cutaneous appendages regeneration in MG and LG. When microcurrent and LLLT were compared, no difference was detected, except the regeneration and formation of new cutaneous appendages, observed in MG. Despite the similar effects, GM showed faster tissue repair with the formation of skin appendages.
关键词： low-level laser therapy,electric stimulation therapy,wound healing
更新于2025-09-12 10:27:22
-
A light-triggerable nanoparticle library for the controlled release of non-coding RNAs
摘要： RNA-based therapies offer a wide range of therapeutic interventions including for the treatment of skin diseases; however, the strategies to deliver efficiently these biomolecules are still limited due to obstacles related to the cellular uptake and cytoplasmic delivery. Herein, we synthesized a triggerable polymeric nanoparticle (NP) library composed by 160 formulations, presenting physico-chemical diversity and differential responsiveness to light. Six formulations were more efficient (up to 500%) than commercial Lipofectamine in gene knockdown activity. These formulations had differential internalization by skin cells and the endosomal escape was rapid (minutes range) as shown by the recruitment of galectin-8. The NPs were effective in the release of siRNA and miRNA. Acute skin wounds treated with the top hit NP complexed with miRNA-150-5p healed faster than wounds treated with scramble miRNA. Light-activatable NPs offer a new strategy to deliver topically non-coding RNAs.
关键词： gene knockdown,wound healing,light-triggerable nanoparticles,endosomal escape,RNA-based therapies
更新于2025-09-12 10:27:22
-
Ablative Fractional Laser Resurfacing
摘要： Normal wound healing is a well-orchestrated process that occurs in 3 characteristic overlapping phases after injury: in?ammation, proliferation, and remodeling/maturation. During the proliferation phase of healing, granulation tissue grows from the base of the wound, ?lling the volume de?cit of the wound, and facilitating epithelialization and wound closure. Aberrantly prolonged stimulation of ?broplasia and angiogenesis, believed to be caused by an imbalance of matrix metalloproteinases, results in hyper-granulation, or “proud ?esh.” Risk factors contributing to the development of hypergranulation include wounds that heal by secondary intention, a prolonged (>1 week) in?ammatory phase of healing, or exposure to excessive external friction or moisture. Hypergranulation is clinically identi?able as red, friable, exophytic tissue that spills over the edges of the wound. Its presence impedes normal wound healing, resulting in persistent wounds that diminish quality of life (pain, bleeding, or malodor), contributing to development of contractures in burn wounds and grafts, and increasing risk of subsequent infection. Treatment is often dif?cult, and multiple modalities have been reported in the literature with varying success including chemical cautery with silver nitrate or tricholoracetic acid, both intralesional or topical corticosteroids, hydrocolloid dressings, surgical excision, pulsed dye laser (PDL), and potassium titanyl phosphate (KTP) laser.1,2 Treatment of surgical, traumatic, and burn scars with the ablative fractional CO2 laser is a recent, highly successful development.3 This infrared laser targets water and vaporizes vertical microcolumns of tissue, creating a zone of coagulation surrounding the ablated microcolumns. After this controlled microscopic injury, a sequential and predictable induction of growth factors and cytokines occurs, leading to a coordinated expression of matrix metalloproteinases, growth factors, and heat-shock proteins.4 Three recently reported case series detail the successful treatment of 10 patients with chronic ulcers with ablative fractional laser resurfacing.3–5 In this report, we describe the successful treatment of 2 patients with hypergranulation tissue using ablative fractional laser resurfacing. To the best of our knowledge, this has not previously been described in the literature.
关键词： Ablative Fractional Laser Resurfacing,Pulsed Dye Laser,CO2 Laser,Hypergranulation Tissue,Wound Healing
更新于2025-09-12 10:27:22
-
Quantitative and qualitative evaluation of recovery process of a 1064?nm laser on laser-induced skin injury: <i>in vivo</i> experimental research
摘要： Laser biological effects are a hot topic in laser medicine. In this study, to explore the quantitative biological effect of laser-induced wound healing and to provide guidance for expanding the clinical application of laser therapy, the injury effects and repair characteristics of skin tissue are studied through infrared laser irradiation of the skin of miniature pigs. Live pig skin was irradiated at multiple spots one time by using a grid-array method with a 1064 nm laser at different power outputs. The skin injury reaction was observed immediately after laser irradiation from low to high doses. The incidence of skin injury was calculated quantitatively. The healing and pathological changes after laser-induced skin injury were observed dynamically within 6 h and for 28 d after laser irradiation. With the increase of irradiation dose, laser-induced skin injuries ranging from mild to severe appeared in turn. The damage threshold of laser irradiation ED50 is 47.4 J cm?2 with the laser; from 3 d to 28 d after irradiation, the pathological results showed that wound healing tended to be different in all groups, but this trend weakened with the increase in laser irradiation intensity. With the increased irradiation dose, skin injury appears as different types of injury plaques, ranging from mild to severe. Skin injury is worsened and the tissue repair trend is weakened with the increase in laser irradiation dose, producing a good dose-effect and time-effect relationship.
关键词： laser-induced skin wound,infrared (IR) laser,wound healing,in vivo laser experiment
更新于2025-09-11 14:15:04
-
NIR‐Laser‐Controlled Hydrogen‐Releasing PdH Nanohydride for Synergistic Hydrogen‐Photothermal Antibacterial and Wound‐Healing Therapies
摘要： For decades, hydrogen (H2) gas has been recognized as an excellent antioxidant molecule that holds promise in treating many diseases like Alzheimer’s, stroke, cancer, and so on. For the first time, active hydrogen is demonstrated to be highly efficient in antibacterial, antibiofilm, and wound-healing applications, in particular when used in combination with the photothermal effect. As a proof of concept, a biocompatible hydrogen-releasing PdH nanohydride, displaying on-demand controlled active hydrogen release property under near-infrared laser irradiation, is fabricated by incorporating H2 into Pd nanocubes. The obtained PdH nanohydride combines both merits of bioactive hydrogen and photothermal effect of Pd, exhibiting excellent in vitro and in vivo antibacterial activities due to its synergistic hydrogen-photothermal therapeutic effect. Interestingly, combinational hydrogen-photothermal treatment is also proved to be an excellent therapeutic methodology in healing rats’ wound with serious bacterial infection. Moreover, an in-depth antibacterial mechanism study reveals that two potential pathways are involved in the synergistic hydrogen-photothermal antibacterial effect. One is to upregulate bacterial metabolism relevant genes like dmpI, narJ, and nark, which subsequently encode more expression of oxidative metabolic enzymes to generate substantial reactive oxygen species to induce DNA damage and another is to cause severe bacterial membrane damage to release intracellular compounds like DNA.
关键词： wound healing,hydrogen releasing materials,synergistic hydrogen-photothermal therapy,antibacterial mechanism,antibacterial activity
更新于2025-09-11 14:15:04
-
Effects of Pulsed 810?nm Al‐Ga‐As Diode Laser on Wound Healing Under Immunosuppression: A Molecular Insight
摘要： Background and Objectives: Dysregulated inflammation is one of the major contributing factors for the prevalence of non‐healing chronic wound in immunosuppressed subjects. Photobiomodulation (PBM) has emerged as a potential non‐thermal, light‐based therapeutic healing intervention for the treatment of impaired wounds. Study Design/Materials and Methods: The present study delineates the underlying molecular mechanisms of PBM 810 nm laser‐induced full‐thickness cutaneous wound repair in immunosuppressed rats at continuous and pulsed wave‐mode with power‐density of 40 mW/cm2, fluence 22.6 J/cm2 for 10 minutes daily for 7 post‐wounding days. Molecular markers were assessed using biochemical, assay quantification, enzyme kinetics and immunoblots analyses pertaining to inflammation, oxidative stress, cell survival, calcium signaling, and proliferation cascades. Results: Results distinctly revealed that pulsed 810 nm (10 Hz) PBM potentially influenced the cell survival and proliferation signaling pathway by significantly upregulated phospho‐protein kinase B(phospho‐Akt), phospho‐extracellular‐signal‐regulated kinase 1 (ERK1), transient receptor potential vanilloid‐3 (TRPV3), Ca2+, calmodulin, transforming growth factor‐β1 (TGF‐β1), TGF‐βR3, and Na+/K+‐ATPase pump levels. PBM treatment resulted in reduction of exaggerated inflammatory responses evident by significantly repressed levels of interleukin‐1β (IL‐1β), IL‐6, cyclooxygenase 2 (COX‐2), and substance‐P receptor (SPR), as well as inhibited apoptotic cell death by decreasing p53, cytochrome C, and caspase 3 levels (P < 0.05), which, in turn, effectively augment the wound repair in immunosuppressed rats. PBM treatment also lowered 4‐hydroxynoneal (HNE) adduct level and NADP/NADPH ratio and upregulated the GRP78 expression, which might culminate into reduced oxidative stress and maintained the redox homeostasis. Conclusions: Taken together, these findings would be helpful in better understanding of the molecular aspects involved in pulsed 810 nm laser‐mediated dermal wound healing in immunosuppressed rats through regulation of cell survival and proliferation via Ca2+‐calmodulin, Akt, ERK, and redox signaling.
关键词： immunosuppressed,dermal wound healing,inflammation,TRPV3,cell survival,TGF‐β,proliferation signaling cascade,photobiomodulation
更新于2025-09-11 14:15:04
-
Polarization property of high harmonics generated from nitrogen molecule by bichromatic counter-rotating circularly polarized laser fields
摘要： Along with the rapid appearance of superbacteria with multidrug resistance, it is a challenge to develop new antibacterial materials to address this big issue. Herein, we report a novel amine group-modi?ed fullerene derivative (C70?(EDA)8), which reveals a high performance in killing superbacteria, and most importantly, it shows negligible toxicity to the mammalian cells. The strong antibacterial ability of this material was attributed to its unique molecular structure. On one hand, amino groups on the EDA part make it easy to a?x onto the outer membrane of multidrug resistance Escherichia coli by electrostatic interactions. On the other hand, the hydrophobic surface on the C70 part makes it easy to form a strong hydrophobic interaction with the inner membrane of bacteria. Finally, C70?(EDA)8 is nontoxic for mammalian cells due to di?erent distributions of the negative charges in the cell membrane. In vivo studies indicated that C70?(EDA)8 mitigated bacterial infection and accelerated wound healing by regulating the immune response and secretion of growth factors. Our amine group-based fullerene derivatives are promising for clinical treatment of wound infection and o?er a new way to ?ght against the superbacteria.
关键词： cytoprotection,wound healing,selectivity,superbacteria,sterilization mechanism,amino fullerene
更新于2025-09-11 14:15:04
-
A light‐triggerable nanoparticle library for the controlled release of non‐coding RNAs
摘要： RNA-based therapies offer a wide range of therapeutic interventions including for the treatment of skin diseases; however, the strategies to deliver efficiently these biomolecules are still limited due to obstacles related to the cellular uptake and cytoplasmic delivery. Herein, we synthesized a triggerable polymeric nanoparticle (NP) library composed by 160 formulations, presenting physico-chemical diversity and differential responsiveness to light. Six formulations were more efficient (up to 500%) than commercial Lipofectamine in gene knockdown activity. These formulations had differential internalization by skin cells and the endosomal escape was rapid (minutes range) as shown by the recruitment of galectin-8. The NPs were effective in the release of siRNA and miRNA. Acute skin wounds treated with the top hit NP complexed with miRNA-150-5p healed faster than wounds treated with scramble miRNA. Light-activatable NPs offer a new strategy to deliver topically non-coding RNAs.
关键词： light-triggerable nanoparticles,siRNA,non-coding RNAs,wound healing,miRNA
更新于2025-09-11 14:15:04
-
The Impacts of Low-Level Laser Therapy – A Complementary Treatment in the Management of Side Effects After Implant Surgery
摘要： Introduction: One of the most important medical applications of laser is low-level laser therapy (LLLT). In this method, laser radiation penetrates easily into the target tissue. The aim of this study was to investigate whether LLLT can reduce the side effects of advanced implant surgery. Methods: In this triple-blind clinical trial, 30 patients aged 25 to 65 years were selected for implant insertion and divided into two groups. In the laser group, immediately after the surgery, 72 hours and 1 week after the surgery, the surgical site was irradiated with an 830 nm laser. The dose required for the laser therapy was 5 J/cm2. The degree of pain, facial swelling and wound healing were analyzed using statistical methods. Results: Our results showed that at 12, 24, 48, and 72 hours after the surgery, the pain level was reduced in the laser group compared with the placebo group (P < 0.05). Swelling of the face was also significantly reduced 7 days after surgery in the laser group (P < 0.05). The investigation on the recovery conditions of the surgical site showed that on the 3rd, 7th and 14th days after the surgery, higher levels of wound healing have been achieved (P < 0.05). Conclusion: Our results suggest that laser, as a complementary therapy; can be used to reduce the severity and duration of pain. Also, laser can reduce facial swelling and accelerate wound healing.
关键词： Side effects,Sinus lift,Implant failure,Low-level laser therapy,Wound healing
更新于2025-09-11 14:15:04
-
Extracellular Collagen Promotes Interleukin-1β–Induced Urokinase-Type Plasminogen Activator Production by Human Corneal Fibroblasts
摘要： PURPOSE. Keratocytes maintain homeostasis of the corneal stroma through synthesis, secretion, and degradation of collagen fibrils of the extracellular matrix. Given that these cells are essentially embedded in a collagen matrix, keratocyte–collagen interactions may play a key role in regulation of the expression or activation of enzymes responsible for matrix degradation including urokinase-type plasminogen activator (uPA), plasmin, and matrix metalloproteinases (MMPs). We examined the effect of extracellular collagen on the production of uPA by corneal fibroblasts (activated keratocytes) stimulated with the proinflammatory cytokine interleukin-1b (IL-1b). METHODS. Human corneal fibroblasts were cultured either on plastic or in a three-dimensional gel of type I collagen. Plasminogen activators were detected by fibrin zymography, whereas the IL-1 receptor (IL-1R) and MMPs were detected by immunoblot analysis. Collagen degradation by corneal fibroblasts was assessed by measurement of hydroxyproline in acid hydrolysates of culture supernatants. RESULTS. Collagen and IL-1b synergistically increased the synthesis and secretion of uPA in corneal fibroblasts. Collagen also upregulated IL-1R expression in the cells in a concentration-dependent manner. The conversion of extracellular plasminogen to plasmin, as well as the plasminogen-dependent activation of MMP-1 and MMP-3 and degradation of collagen apparent in three-dimensional cultures of corneal fibroblasts exposed to IL-1b, were all abolished by a selective uPA inhibitor. CONCLUSIONS. Collagen and IL-1b cooperate to upregulate uPA production by corneal fibroblasts. Furthermore, IL-1b–induced collagen degradation by these cells appears to be strictly dependent on uPA expression and mediated by a uPA–plasmin–MMP pathway.
关键词： corneal wound healing,urokinase,collagen degradation
更新于2025-09-11 14:15:04