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Photobiomodulation Improved the First Stages of Wound Healing Process After Abdominoplasty: An Experimental, Double-Blinded, Non-randomized Clinical Trial
摘要： Background Photobiomodulation is widely studied for its potential benefits in the wound healing process. Numerous scientific studies have highlighted its effect on various phases of wound repair, but clinical validations are few. This comparative trial aims to evaluate the influence of photobiomodulation on the post-abdominoplasty healing process. Methods Seventeen Caucasian women (aged 18–55) who underwent an abdominoplasty were enrolled in this double-blinded, controlled clinical trial. The postoperative scars were divided into two areas; the right side of the scars was treated with ten sessions of photobiomodulation (consisting in three types of wavelengths). The other part of the scars was used as control and did not receive any additional treatment. Clinical assessments of both parts of the scars were scheduled at 1, 6 and 12 months postoperative. Results Within six months following surgery, significantly improved quality of the scars on the treated side compared with the untreated side was reported by patients and experienced professionals according to Vancouver Scar Scale, Patient and Observer Scar Assessment Scale (p < 0.05) and standardized photographs (p < 0.05). At 1 year of follow-up, patients observed no differences between the treated and untreated sides of the scars. This suggests that photobiomodulation appears to play an early role in the wound healing process, accelerating the first stages of cicatrization. Conclusion This study statistically validates the positive impact of photobiomodulation treatment on the first stages of the postoperative healing process. Carried out on Caucasians participants only, this study should, however, be performed on a more heterogeneous population to definitively confirm these effects on an international population.
关键词： Photobiomodulation,Wound healing,Surgical scar,Abdominoplasty,Phototherapy
更新于2025-09-09 09:28:46
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The Possible Limitation of the Intra-Operative PDT Dosimetry: On Wounds Healing, Following Tumor Resection
摘要： In the past three decades, laser research in Saudi Arabia had provided methodical findings for the advancement of laser application in medicine. At low power emission, lasers incite photochemical effects, which diverge into two inevitable therapeutic phenomena Photo Dynamic Therapy (PDT) and Low Power Laser Therapy (LPLT). The term PDT refers to the combined use of a Photo Sensitizer (PS) (Dye) plus Laser to treat and diagnose malignant cells. In the doses usually used for treatments, the dye alone or the laser alone has no effect. PDT, a laser treatment for cancer, involves light and PS, (i.e. a light-sensitive drug, for instance – Hemato Porphyrin Derivative (HPD), Photofrin, and 5-Aminolevulinic Acid etc.) for the selective destruction of Neoplastic cells. Rigorous PDT studies in our laboratory are ongoing since 1985 aiming to determine the most suitable laser wavelength and PS, in thyroid tumor using nude mice as animal models. Previously we have established the Clonogenic Survival Rates, and tumor growths following PDT as a function of tumor volume for RIF-1 (murine fibro sarcoma) in C3H mice. We were able to completely eradicate small tumors (i.e. tumor volume < 50 mm3), and decrease the growth rates of larger tumors by executing superficial PDT followed by interstitial PDT. Thus, unlike ionizing radiation, PDT may be repeated if necessary without yielding cumulative toxicity [1-4]. The high laser doses required for the presents widely used dye {Photofrin @ I and II (HPD)}, may result, as our recent experiments show, in inhibiting Wound Healing of up to 8%. Where if the PDT laser dose decreased it could stimulate acceleration of wound healing up to 30%.Where the Incident dose for PDT with Photofrin of 80 J/cm2 give zero bio activation for wound healing. With regard to LPLT, we study its effects on wounds and burns in Sprague-Dawley rats. We noticed healing acceleration, zero-bio activation, and inhibition that depended on the wavelength, dose and treatment schedule, but not on dose rate and laser-skin transmission. We further observed that LPLT had higher wound healing acceleration than Light Emitting Diode (LED), or pharmaceutical treatments. We found 29% healing acceleration with HeNe (632.8 nm) laser at 5 J/cm2 (human dose) 40 J/cm2 (animal dose), applied three times a week. 42% of the animals had better cosmos’s .When wound healing was impaired by diabetes, LPLT accelerated healing by +8.6% relative to diabetic controls (-42%) [5-8]. This support the suggestion of using other dyes (new PS, developed to overcome some of other problems, like unacceptable prolongation of the procedure if large area treated and poor absorption of light By HPD). Since then several classes of PS were developed. The main classes are: porphyrin derivatives, Chlorins, Phthalocyanines and Porphycenes etc., Must have the properties of high absorption of laser, and deep tissue penetrations. Also these dyes should use a simulative laser dose for the wound healing, as the PDT optimum dose when combined with surgery, beside its efficacy for selectively destroying malignant tissue. The laser incident dose For PDT suggested from our experiments is up to 40 J/cm2 to give also wound healing acceleration in the animal, (which should converted to human),and up to 140 J/cm2 for deceleration in the animal. We advocate the Intraoperative Photo Dynamic Therapy (IPDT). Initially, surgical lasers excise/ablate visible tumors sealing the lymph vessels in the process thereby preventing further metastasis. Highly selective PDT follows removing microscopic residual cancer cells that remain after the surgery. Finally, LPLT is applied to accelerate healing of postoperative normal wounds (and /or Diabetic wounds). In time, medical lasers shall supersede some conventional therapies simply because of being overwhelmingly effective in various clinical applications.
关键词： Stimulation,HPD,Photo-bio modulation,Photo dynamic therapy,Low power laser therapy,Tumors,Photo sensitizer,Inhibition,Wound healing
更新于2025-09-04 15:30:14
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Tissue-derived microparticles reduce inflammation and fibrosis in cornea wounds
摘要： Biological materials derived from the extracellular matrix (ECM) of tissues serve as scaffolds for rebuilding tissues and for improved wound healing. Cornea trauma represents a wound healing challenge as the default repair pathway can result in fibrosis and scar formation that limit vision. Effective treatments are needed to reduce inflammation, promote tissue repair, and retain the tissue’s native transparency and vision capacity. Tissue microparticles derived from cornea, cartilage and lymph nodes were processed and screened in vitro for their ability to reduce inflammation in ocular surface cells isolated from the cornea stroma, conjunctiva, and lacrimal gland. Addition of ECM particles to the media reduced expression of inflammatory genes and restored certain tear film protein production in vitro. Particles derived from lymph nodes were then applied to a rabbit lamellar keratectomy corneal injury model. Application of the tissue particles in a fibrin glue carrier decreased expression of inflammatory and fibrotic genes and scar formation as measured through imaging, histology and immunohistochemistry. In sum, immunomodulatory tissue microparticles may provide a new therapeutic tool for reducing inflammation in the cornea and ocular surface and promoting tissue repair.
关键词： ocular surface,inflammation,Extracellular matrix,corneal wound healing,corneal fibrosis
更新于2025-09-04 15:30:14
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Corneal Fibroblast Migration Patterns During Intrastromal Wound Healing Correlate With ECM Structure and Alignment
摘要： PURPOSE. To assess keratocyte backscattering, alignment, morphology, and connectivity in vivo following a full-thickness corneal injury using the Heidelberg Retina Tomograph Rostock Cornea Module (HRT-RCM), and to correlate these findings with en bloc three-dimensional (3-D) confocal fluorescence and second harmonic generation (SHG) imaging. METHODS. Rabbit corneas were scanned in vivo both before and 3, 7, 14, and 28 days after transcorneal freeze injury (FI), which damages all corneal cell layers. Corneal tissue was also fixed and labeled for f-actin and nuclei en bloc, and imaged using 3-D confocal fluorescence microscopy and SHG imaging. RESULTS. Using the modified HRT-RCM, full-thickness scans of all cell layers were consistently obtained. Following FI, stromal cells repopulating the damaged tissue assumed an elongated fibroblastic morphology, and a significant increase in cellular light scattering was measured. This stromal haze gradually decreased as wound healing progressed. Parallel, interconnected streams of aligned corneal fibroblasts were observed both in vivo (from HRT-RCM reflection images) and ex vivo (from f-actin and nuclear labeling) during wound healing, particularly in the posterior cornea. Second harmonic generation imaging demonstrated that these cells were aligned parallel to the collagen lamellae. CONCLUSIONS. The modified HRT-RCM allows in vivo measurements of sublayer thickness, assessment of cell morphology, alignment and connectivity, and estimation of stromal backscatter during wound healing. In this study, these in vivo observations led to the novel finding that the pattern of corneal fibroblast alignment is highly correlated with lamellar organization, suggesting contact guidance of intrastromal migration that may facilitate more rapid wound repopulation.
关键词： SHG imaging,confocal microscopy,corneal wound healing,extracellular matrix
更新于2025-09-04 15:30:14