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Synergistic Effects of Photo-Irradiation and Curcumin-Chitosan/Alginate Nanoparticles on Tumor Necrosis Factor-Alpha-Induced Psoriasis-Like Proliferation of Keratinocytes
摘要： Psoriasis is a chronic inflammatory skin disease characterized by hyperproliferation of the epidermal cells and is clinically presented as thick, bright red to pink plaques with a silvery scale. Photodynamic therapy (PDT) using visible light has become of increasing interest in the treatment of inflammatory skin diseases. In this study, we demonstrate that a combination of curcumin-loaded chitosan/alginate nanoparticles (Cur-CS/Alg NPs) and blue light emitting diodes (LED) light irradiation effectively suppressed the hyperproliferation of tumor necrosis factor-alpha (TNF-α)-induced cultured human kerlatinocyte (HaCaT) cells. The Cur-CS/Alg NPs were fabricated by emulsification of curcumin in aqueous sodium alginate solution and ionotropic gelation with calcium chloride and chitosan using an optimized formulation derived from a Box-Behnken design. The fabricated Cur-CS/Alg NPs were characterized for their particle size, zeta potential, encapsulation efficiency, and loading capacity. The surrogate 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, to measure the relative number of viable cells, showed that the CS/Alg NPs were nontoxic to normal HaCaT cells, while 0.05 μg/mL and 0.1 μg/mL of free curcumin and Cur-CS/Alg NPs inhibited the hyperproliferation of HaCaT cells induced by TNF-α. However, the Cur-CS/Alg NPs demonstrated a stronger effect than the free curcumin, especially when combined with blue light irradiation (10 J/cm2) from an LED-based illumination device. Therefore, the Cur-CS/Alg NPs with blue LED light could be potentially developed into an effective PDT system for the treatment of psoriasis.
关键词： LED light,chitosan/alginate nanoparticles,curcumin,photo-irradiation,psoriasis
更新于2025-11-19 16:56:35
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A thermo-responsive alginate nanogel platform co-loaded with gold nanoparticles and cisplatin for combined cancer chemo-photothermal therapy
摘要： The current interest in cancer research is being shifted from individual therapy to combinatorial therapy. In this contribution, a novel multifunctional nanoplatform comprising alginate nanogel co-loaded with cisplatin and gold nanoparticles (AuNPs) has been firstly developed to combine photothermal therapy and chemotherapy. The antitumor efficacy of the as-prepared nanocomplex was tested against CT26 colorectal tumor model. The nanocomplex showed an improved chemotherapy efficacy than free cisplatin and caused a significantly higher tumor inhibition rate. The in vivo thermometry results indicated that the tumors treated with the nanocomplex had faster temperature rise rate under 532 nm laser irradiation and received dramatically higher thermal doses due to optical absorption properties of AuNPs. The combined action of chemo-photothermal therapy using the nanocomplex dramatically suppressed tumor growth up to 95% of control and markedly prolonged the animal survival rate. Moreover, tumor metabolism was quantified by [18F]FDG (2-deoxy-2-[18F]fluoro-D-glucose)-positron emission tomography (PET) imaging and revealed that the combination of the nanocomplex and laser irradiation have the potential to eradicate microscopic residual tumor to prevent cancer relapse. Therefore, the nanocomplex can afford a potent anticancer efficacy whereby heat and drug can be effectively deliver to the tumor, and at the same time the high dose-associated side effects due to the separate application of chemotherapy and thermal therapy could be potentially reduced.
关键词： Alginate,Cisplatin,Gold nanoparticles,Chemo-photothermal therapy,Positron emission tomography
更新于2025-09-23 15:22:29
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Optogenetic control of iPS cell‐derived neurons in 2D and 3D culture systems using channelrhodopsin‐2 expressed by the SYN1 and CaMKII promoters
摘要： Development of optogenetically controllable human neural network model in three-dimensional (3D) can provide an investigative system that is physiologically relevant or mimic to the human brain. Light-sensitive neurons were generated by transducing channelrhodopsin-2 (ChR2) into human induced pluripotent stem cells (hiPSCs) derived neural progenitor cells (Axol) using lentiviruses and cell-type specific promoters. A mixed population of human iPSC-derived cortical neurons, astrocytes and progenitor cells was obtained (Axol-ChR2) upon neural differentiation. Pan-neuronal promoter synapsin-1 (SYN1) and excitatory neuron-specific promoter calcium-calmodulin kinase II (CaMKII) were used to drive reporter gene expression in order to assess the differentiation status of the targeted cells. Expression of ChR2 and characterisation of sub-populations in differentiated Axol-ChR2 cells were evaluated using flow cytometry and immunofluorescent staining. These cells were transferred from 2D culture to 3D alginate hydrogel functionalised with arginine-glycine-aspartate (RGD) and small molecules (Y-27632). Improved RGD-alginate hydrogel was physically characterised and assessed for cell viability to serve as a generic 3D culture system for hPSCs and neuronal cells. Prior to cell encapsulation, neural network activities of Axol-ChR2 cells and primary neurons were investigated using calcium imaging. Results demonstrate that functional activities were successfully achieved through expression of ChR2- by both the CaMKII and SYN1 promoters. The RGD-alginate hydrogel system supports the growth of differentiated Axol-ChR2 cells whilst allowing detection of ChR2 expression upon light stimulation. This allows precise and non-invasive control of human neural networks in 3D.
关键词： channelrhodopsin-2 (ChR2),Optogenetics,induced pluripotent stem cell (iPSC),3D culture,calcium-calmodulin kinase II (CaMKII),synapsin-1 (SYN1),alginate hydrogel,neural tissue engineering
更新于2025-09-23 15:19:57
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Stimuli‐Responsive Luminescent Copper Nanoclusters in Alginate and Their Sensing Ability for Glucose
摘要： Visually observable pH-responsive luminescent materials are developed through integrating the properties of aggregation-induced emission enhancement of Cu nanocluster (NCs) and the Ca2+ triggered gelatin of alginate. Sodium alginate, CaCO3 nanoparticles and Cu NCs are dispersed in aqueous solution, which is in a transparent fluid state, showing a weak photoluminescence (PL). The introduced H+ can react with the CaCO3 nanoparticles to produce free Ca2+, which can cross-link the alginate chains into gel networks. Meanwhile, a dramatically increase on the PL intensity of Cu NCs and a blue shift on the PL peak appeared, assigned to the Ca2+ induced enhancement and gelatin induced enhancement, respectively. Their potential application as a sensor for glucose is also demonstrated based on the principle that glucose oxidase can recognize glucose and produce H+, which further triggers the above mentioned two-stage enhancement. A linear relationship between the PL intensity and concentration of glucose in the range of 0.1 to 2.0 mM is obtained, with a limit of detection calculated as 3.2×10-5 M.
关键词： alginate,stimuli‐responsive materials,aggregation-induced emission,photoluminescence,metal nanoclusters,glucose
更新于2025-09-19 17:15:36
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Preparing CdS QDs in sodium alginate gel: realizing water-solubility and stimuli-responsiveness of QDs in an integrative way
摘要： Quantum dots (QDs) were of great interest due to the excellent fluorescence property and have been widely studied. Comparing with the typical organometallic synthetic routes and hydrothermal method that were usually carried out under high temperature, colloidal template could be used for preparing QDs in mild conditions and have gained increasing attention. In this prospect, hydrogel was an ideal colloidal template for preparation of QDs in aqueous medium while the related study for in-situ preparation of QDs in gel and the consequent functionalization of QDs was in demand. In this paper, we proposed a two-step method to prepare CdS QDs in sodium alginate (SA) gel which showed effective constraint in uniform size distribution of QDs. Without extra ligands introduction, the prepared CdS-SA QDs showed responsiveness to pH and detectability to Fe3+ which provide a simplified way for functionalization of QDs. The CdS-SA QDs showed good biocompatibility and stability in a certain concentration which indicated the application prospect of the CdS-SA QDs in fields of biological labeling and environmental sensor.
关键词： gel,stimuli responsiveness,pH,Sodium alginate,CdS QDs
更新于2025-09-19 17:15:36
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Layer-by-Layer-Assembled Antifouling Films with Surface Microtopography Inspired by Laminaria japonica
摘要： Marine biofouling is an important obstacle to the development of marine resources. Problems such as huge economic losses caused by biofouling and environmental damage caused by toxic antifouling agents have not been resolved. Therefore, it is of great significance to develop a novel, environmentally friendly antifouling (AF) materials. However, in the marine environment, Laminaria japonica still has excellent antifouling ability in a relatively static state compared to those parade creatures. Inspired by this, this study reports a synergistic effect between surface topography and chemical modification to inhibit marine biofouling. Firstly, the surface of the Laminaria japonica was analyzed and its morphology was reproduced using a simple moulding process. Additionally, the polyelectrolyte layer composed of sodium alginate and (guanidine-hexamethylenediamine-PEI) (poly(GHPEI)) was chemically modified on the isotropic microstructure surface of PDMS replicas by layer-by-layer assembly method. The anti-adhesion ability of the biomimetic material was tested with Nitzschia closterium (N. closterium) (9±5 diatoms mm-2 of N. closterium adhered). Meanwhile, the antifouling performance of the modified films were evaluated by Escherichia coli (E. coli), and its antibacterial ability were as high as 96.2±1.3%. The combination of microtopography and (GHPEI/ALG) * n films endow the coating with excellent antifouling ability.
关键词： marine antifouling,surface microtopography,layer-by-layer assembly,alginate,poly(GHPEI),chemical modifications
更新于2025-09-16 10:30:52
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Synchronous enhancement and stabilization of graphene oxide liquid crystals: Inductive effect of sodium alginates in different concentration zones
摘要： A facile way to simultaneously induce the formation and enhance the stability of graphene oxide liquid crystals (GO LCs) is reported. Sodium alginate (SA) with both inductive effect and stabilization effect was added into GO suspensions to prepare LCs at low concentration that could survive harsh conditions. Most importantly, different concentration zones of SA brought significantly different LCs degree and stability, which possessed distinct charge properties, rheological behaviors and chain morphology. Adding 0.5 % SA located in entangled semi-dilute regime could induce the isotropic-nematic transition of GO at low concentration (< 0.035 %) and showed excellent liquid crystalline behavior due to the enhanced electrostatic repulsion of GO/SA colloids, while 1% SA located in concentrated solution regime brought better tolerance to NaCl, MgCl2, FeCl3, serum and pH of 1-13 due to the stronger bounding effect of entanglement network structure to countra-ions. This work develops a simple and eco-friendly method to prepare stable GO LCs at low GO concentration with application potentials especially in biomedical field, and the relationship between the concentration zones of SA and LCs behavior offers theoretical guidance for the self-assembly of GO macrostructures and design and efficient processing of its composites.
关键词： Sodium alginate,Liquid crystals,Graphene oxide
更新于2025-09-10 09:29:36
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Alginate-graphene oxide hydrogels with enhanced ionic tunability and chemomechanical stability for light-directed 3D printing
摘要： Nanocomposite hydrogels that incorporate 2D carbon nanomaterials could enable augmented and responsive behaviors not observed with polymeric matrices alone. In particular, non-covalent interactions could facilitate enhanced mechanical performance that can be self-recovered with external stimuli. Here, we demonstrate alginate-graphene oxide (GO) hydrogels using a non-covalent, ionic crosslinking mechanism compatible with light-directed 3D printing. We show that alginate-GO hydrogels exhibit improved mechanical performance in shear, compression, and tension, including a two-fold increase in shear modulus, a three-fold decrease in inelastic deformation, and a nine-fold increase in fracture energy relative to alginate-only hydrogels. Moreover, alginate-GO hydrogels are stabilized by hydrogen bonding between nanosheets and remain intact after removal of ionic crosslinkers by chelation. As a consequence, the shear modulus of these nanocomposite hydrogels can be tuned by over 500-fold via external ion concentration. We demonstrate that alginate-GO can be stereolithographically printed into robust, freestanding and overhanging 3D structures. These designer material architectures exhibit outstanding stability and superoleophobicity in high salt solution, which can be used to repel and manipulate a variety of oils. Overall, such nanocomposite hydrogels with engineered non-covalent interactions could enable “smart” multiresponsive and multifunctional devices for aqueous and marine environments.
关键词： Superoleophobicity,Ionic crosslinking,Chemomechanical stability,Graphene oxide,3D printing,Alginate,Nanocomposite hydrogels,Mechanical performance
更新于2025-09-10 09:29:36
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pH Variation as a Simple and Selective Pathway for Obtaining Nanoparticle or Nanocapsule Polysaccharides
摘要： The fabrication of polysaccharides to be nanoparticles or nanocapsules is quite specific due to various parameters and factors. The present work demonstrates a simple pathway to selectively prepare the ionic polysaccharide flakes to be nanoparticles or nanocapsules. The systematic studies on the model cases of cationic polysaccharide (i.e. chitosan) and anionic polysaccharide (i.e. alginate) confirm that pKa is the key point to tune the polysaccharides to be nanoparticles or nanocapsules. When the ionic polysaccharides were in an oil/water emulsion system, the pH close to pKa leads to the densely packed polysaccharide chains under the hydrogen bond networks, and as a result the crosslink occurs all through the chains to be nanoparticles. On the other hand, when pH was adjusted to the lower or higher than pKa depending on the types of ionic polysaccharide, the polysaccharide chains are under charge-charge repulsive force, resulting in the alignment of polysaccharide chains to be hollow nanospheres, and at that time the crosslink initiates the formation of nanocapsules. The present work, for the first time, clarifies that pH variation is the key to selectively prepare nanoparticles or nanocapsules, and this is important for delivery systems, coatings, sensors, etc.
关键词： pH-tunable morphology,chitosan,alginate,emulsion system,Polysaccharide
更新于2025-09-10 09:29:36
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AIP Conference Proceedings [Author(s) PROCEEDINGS OF THE 3RD INTERNATIONAL SYMPOSIUM ON CURRENT PROGRESS IN MATHEMATICS AND SCIENCES 2017 (ISCPMS2017) - Bali, Indonesia (26–27 July 2017)] - Synthesis and photocatalytic activity of nanocomposite based on sodium alginate from brown algae with ZnO impregnation
摘要： The objective of this research is to synthesize nano sodium alginate-ZnO nanocomposite as photocatalyst to degrade methylene blue compounds. The successful synthesis of nano sodium alginate-ZnO nanocomposite was supported by the characterization of XRD (X-Ray Diffraction), FTIR (Fourier Transform Infrared), SEM (Scanning Electron Microscope) and TEM (Tunneling Electron Microscope). The first stage of the research was the synthesis of nano sodium alginate with particle size 140 nm and crystallinity index 38.78 % by XRD and TEM characterization, the obtained particle size was 146 nm x 61 nm. Nano ZnO particle size 38 nm, by XRD and TEM characterization the obtained particle size was 56.6 nm x 33.3 nm. The next stage of nanocomposite synthesis of nano sodium alginate was the impregnation with nano ZnO particle size 130 nm, by XRD and TEM characterization the particle size was 228.0 nm x 134.2 nm. The success was also supported by FTIR and SEM characterization. The final stage was the degradation of blue methylene using nano-sodium alginate-ZnO nanocomposite. The highest degradation percentage by nano sodium alginate-ZnO nanocomposite with zinc nitrate concentration of 0.075 M was 51.68% with the first order rate constant of 9.5 x10-3 /min.
关键词： methylene blue,nano ZnO,degradation,nanocomposite,nano sodium alginate
更新于2025-09-04 15:30:14