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Fluorescence Spectroscopic Studies of in vitro Interactions of Famotidine and Tapentadol Hydrochloride with Bovine Serum Albumin
摘要： The in vitro interactions of Famotidine (FT) and Tapentadol hydrochloride (TAP) with bovine serum albumin (BSA) have been studied by fluorescence emission spectroscopy under different conditions. Quenching constants were determined using the Stern-Volmer equation. Two moles FT bound with 1 mole of BSA at 298 K and 3 mole FT bound with 1 mole of BSA at 308 K in presence of TAP. BSA was used for the study as it shows approximately 76% sequence homology to human serum albumin (HSA).
关键词： in vitro,fluorescence,bovine serum albumin,Famotidine,Tapentadol Hydrochloride,spectroscopy
更新于2025-09-09 09:28:46
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Quenching of Luminol Fluorescence at Nano-Bio Interface: Towards the Development of an Efficient Energy Transfer System
摘要： Surface modified colloidal gold (Au) and silver (Ag) nanoparticles (NPs) were used as efficient quenchers of luminol (LH2) fluorescence either in homogeneous aqueous medium or its noncovalent assembly with bovine serum albumin (BSA). The mechanism as well as the extent of fluorescence quenching was found to be strongly dependent on the nature of the nanoparticles. While simple static type fluorescence quenching mechanism was perceived with AuNP, a more complex protocol involving quenching sphere model was envisaged for AgNP quenching. Nevertheless, the magnitude of Stern-Volmer (SV) quenching constant (KSV ~ 108–1010 M?1) was calculated to be ca. 104 times more for surface quoted NPs in comparison with BSA–NP bioconjugates system. On the other hand, a highly efficient (E ≈ 95%) energy transfer (ET) process was predicted for LH2 captured in the hydrophobic assembly with BSA in presence of AgNP as an acceptor. The ET efficiency is critically dependent on the concentration of BSA and nicely correlated with the extent of NP surface coverage. However, fluorescence quenching on AuNP surface is relatively less responsive towards protein concentration, primarily due to the difference in surface activity as well as the mode of interaction of the protein with NPs.
关键词： Bionanosensors,Bovine serum albumin,Luminol,Metal nanoparticles,Energy transfer,Fluorescence quenching
更新于2025-09-09 09:28:46