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- 摘要
- 关键词
- 实验方案
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过滤筛选
- 2018
- electromagnetic pulse
- cell proliferation
- cell membrane permeability
- cell response to electromagnetic stress
- apoptosis
- cancer therapy
- necrosis
- Intelligent Medical Engineering
- V.N. Karazin Kharkiv National University
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Oncological and Surgical Outcomes of Patients Treated by Transoral CO <sub/>2</sub> Laser Cordectomy for Early-Stage Glottic Squamous Cell Carcinoma: A Retrospective Chart Review
摘要： Objective: To investigate the feasibility and the outcomes of transoral laser CO2 microsurgery (TLM) for resection of early-stage squamous cell carcinoma (SCC) of the vocal folds through several additional surgical procedures and tips improving the glottic exposure. Methods: Retrospective chart review of patients treated by TLM cordectomy in a single European University Hospital for early-stage vocal fold SCCs (Tis, T1a, T1b, and T2). The following TLM outcomes were studied regarding the tumor size (Tis and T1a vs T1b and T2) and the margin status (negative vs positive/suspicious): patient position; type of laryngoscope; requirement to external counter pressure; resection of supraglottic structures (eg, ventricular band, epiglottic petiole, and suprahyoid epiglottis); pre- and postoperative complications; overall survival; disease-specific survival (DSS); and disease-free survival (DFS). Results: A total of 148 patients were included. The TLM was realized in 95.3% of cases. External counter pressure, partial, or total vestibulectomy were necessary in 65.9%, 57.4%, and 4.2% of cases, respectively. A resection of the epiglottic petiole was required in 24.8% of cases. The realization of both epiglottis petiole resection and vestibulectomies were significantly higher in patients with T2 and T1b SCCs compared to those with T1a and Tis SCCs (P ? .01). Different procedure tips were described for improving the laryngeal exposition. The 5-year laryngeal preservation rate, DSS, and DFS were significantly better in patients without SCC involvement of the anterior commissure, and did not vary according to the margin status. The laryngeal exposure difficulties did not impact the margin status. Conclusion: The exposure of glottis is possible in 95% of cases of early-stage vocal cord SCC but requires the use of several additional surgical procedures, especially for anterior commissure SCCs. The SCC involvement of the vocal fold anterior commissure is associated with lower DSS, DFS, and laryngeal preservation rate.
关键词： vocal,exposure,glottic,margin,laryngeal,fold,transoral laser microsurgery,cancer
更新于2025-09-23 15:21:01
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The near-infrared fluorescent dye IR-780 was coupled with cabazitaxel for castration-resistant prostate cancer imaging and therapy
摘要： A new drug, Caba-780, was synthesized by chemical coupling of the heptamethyl phthalocyanine near-infrared fluorescent (NIRF) dye IR-780 and the paclitaxel-based chemotherapeutic drug cabazitaxel. Then, the potential value of Caba-780 in the diagnosis and treatment of castration-resistant prostate cancer (CRPC) was evaluated. The CRPC cell lines DU145 and PC-3, as well as the normal human prostate stromal cell line WPMY-1, were used to evaluate the uptake of Caba-780 and its antitumor effect in vitro. The distribution, antitumor effect, and safety of Caba-780 were also evaluated in tumor-bearing mouse xenograft models. Our results showed that Caba-780 was efficiently absorbed by DU145 and PC-3 cells and that the cytotoxicity of Caba-780 was significantly stronger than that of IR-780 and cabazitaxel. In addition, Caba-780 inhibited the migration and invasion of DU145 and PC-3 cells and promoted apoptosis by prolonging the G2 phase of the cell cycle. Further analysis indicated that Caba-780 could be used to effectively image tumor xenografts. At the same time, this drug inhibited the growth of tumors in vivo. Therefore, the new synthetic drug Caba-780 has potential applications in the diagnosis and treatment of CRPC.
关键词： Organic anion-transporting polypeptide,Chemotherapy,Castration-resistant prostate cancer,Near-infrared fluorescent dye
更新于2025-09-23 15:21:01
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<sup>86/90</sup> Y-Labeled Monoclonal Antibody Targeting Tissue Factor for Pancreatic Cancer Theranostics
摘要： Pancreatic cancer is highly aggressive, with a median survival time of less than 6 months and a 5-year overall survival rate of around 7%. The poor prognosis of PaCa is largely due to its advanced stage at diagnosis and the lack of efficient therapeutic options. Thus, the development of an efficient, multifunctional PaCa theranostic system is urgently needed. Overexpression of tissue factor (TF) has been associated with increased tumor growth, angiogenesis, and metastasis in many malignancies, including pancreatic cancer. Herein, we propose the use of a TF-targeted monoclonal antibody (ALT836) conjugated with the pair 86/90Y as a theranostic agent against pancreatic cancer. For methods, serial PET imaging with 86Y-DTPA-ALT836 was conducted to map the biodistribution the tracer in BXPC-3 tumor-bearing mice. 90Y-DTPA-ALT836 was employed as a therapeutic agent that also allowed tumor burden monitoring through Cherenkov luminescence imaging. The results were that the uptake of 86Y-DTPA-ALT836 in BXPC-3 xenograft tumors was high and increased over time up to 48 h postinjection (p.i.), corroborated through ex vivo biodistribution studies and further confirmed by Cherenkov luminescence Imaging. In therapeutic studies, 90Y-DTPA-ALT836 was found to slow tumor growth relative to the control groups and had significantly smaller (p < 0.05) tumor volumes 1 day p.i. Histological analysis of ex vivo tissues revealed significant damage to the treated tumors. The conclusion is that the use of the 86/90Y theranostic pair allows PET imaging with excellent tumor-to-background contrast and treatment of TF-expressing pancreatic tumors with promising therapeutic outcomes.
关键词： pancreatic cancer,yttrium-86,radioimmunotherapy,tissue factor,yttrium-90,theranostics,positron emission tomography (PET)
更新于2025-09-23 15:21:01
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Synthesis and analysis of 64Cu-labeled GE11-modified polymeric micellar nanoparticles for EGFR-targeted molecular imaging in a colorectal cancer model
摘要： Polymeric micellar nanoparticles represent versatile and biocompatible platforms for targeted drug delivery. However, tracking their biodistribution, stability, and clearance profile in vivo is challenging. The goal of this study was to prepare surface-modified micelles with peptide GE11 for targeting the epidermal growth factor receptor (EGFR). In vitro fluorescence studies demonstrated significantly higher internalization of GE11-micelles into EGFR-expressing HCT116 colon cancer cells versus EGFR-negative SW620 cells. Azo coupling chemistry of tyrosine residues in the peptide backbone with aryl diazonium salts was used to label the micelles with radionuclide 64Cu for positron emission tomography (PET) imaging. In vivo analysis of 64Cu-labeled micelles showed prolonged blood circulation and predominant hepatobiliary clearance. The biodistribution profile of EGFR-targeting GE11-micelles was compared with non-targeting HW12-micelles in HCT116 tumor-bearing mice. PET revealed increasing tumor-to-muscle ratios for both micelles over 48 h. Accumulation of GE11-containing micelles in HCT116 tumors was higher compared to HW12-decorated micelles. Our data suggest that the efficacy of image-guided therapies with micellar nanoparticles could be enhanced by active targeting, as demonstrated with cancer biomarker EGFR.
关键词： polymeric micelles,epidermal growth factor receptor (EGFR),64Cu,GE11 peptide,Colorectal cancer,positron emission tomography (PET)
更新于2025-09-23 15:21:01
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New Unsymmetrical Bisacridine Derivatives Noncovalently Attached to Quaternary Quantum Dots Improve Cancer Therapy by Enhancing Cytotoxicity toward Cancer Cells and Protecting Normal Cells
摘要： The use of nanoparticles for the controlled drug delivery to cells has emerged as a good alternative to traditional systemic delivery. Quantum dots (QDs) offer potentially invaluable societal benefits such as drug targeting and in vivo biomedical imaging. In contrast, QDs may also pose risks to human health and the environment under certain conditions. Here, we demonstrated that a unique combination of nanocrystals core components (Ag-In-Zn-S) would eliminate the toxicity problem and increase their biomedical applications. The alloyed quaternary nanocrystals Ag-In-Zn-S (QDgreen, Ag1.0In1.2Zn5.6S9.4; QDred, Ag1.0In1.0Zn1.0S3.5) were used to transport new unsymmetrical bisacridine derivatives (UAs, C-2028 and C-2045) into lung H460 and colon HCT116 cancer cells for improving the cytotoxic and antitumor action of these compounds. UAs were coupled with QD through physical adsorption. The obtained results clearly indicate that the synthesized nanoconjugates exhibited higher cytotoxic activity than unbound compounds, especially toward lung H460 cancer cells. Importantly, unsymmetrical bisacridines noncovalently attached to QD strongly protect normal cells from the drug action. It is worth pointing out that QDgreen or QDred without UAs did not influence the growth of cancer and normal cells, which is consistent with in vivo results. In noncellular systems, at pH 5.5 and 4.0, which relates to the conditions of endosomes and lysosomes, the UAs were released from QD-UAs nanoconjugates. An increase of total lysosomes content was observed in H460 cells treated with QDs-UAs which can affect the release of the UAs from the conjugates. Moreover, confocal laser scanning microscopy analyses revealed that QD-UAs nanoconjugates enter H460 cells more efficiently than to HCT116 and normal cells, which may be the reason for their higher cytotoxicity against lung cancer. Summarizing, the noncovalent attachment of UAs to QDs increases the therapeutic efficiency of UAs by improving cytotoxicity toward lung H460 cancer cells and having protecting effects on normal cells.
关键词： lung and colon cancer cells,unsymmetrical bisacridine derivatives,drug-carrier degradation pathway,pH-dependent release,cellular uptake,in vivo antitumor efficacy,Ag-In-Zn-S nanocrystals,cytotoxic activity
更新于2025-09-23 15:21:01
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[IEEE 2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC) - Munich, Germany (2019.6.23-2019.6.27)] 2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC) - Probing Neuronal Activity using Membrane Interfacial Water
摘要： The use of high-volume quantitative radiomics features extracted from multi-parametric magnetic resonance imaging (MP-MRI) is gaining attraction for the autodetection of prostate tumors, since it provides a plethora of mineable data, which can be used for both detection and prognosis of prostate cancer. While current voxel-resolution radiomics-driven prostate tumor detection approaches utilize quantitative radiomics features associated with individual voxels on an independent basis, the incorporation of additional information regarding the spatial and radiomics feature relationships between voxels has significant potential for achieving a more reliable detection performance. Motivated by this, we present a novel approach for automatic prostate cancer detection using a radiomics-driven conditional random field (RD-CRF) framework. In addition to the high-throughput extraction and utilization of a comprehensive set of voxel-level quantitative radiomics features, the proposed RD-CRF framework leverages inter-voxel spatial and radiomics feature relationships to ensure that the autodetected tumor candidates exhibit interconnected tissue characteristics reflective of cancerous tumors. We evaluated the performance of the proposed framework using clinical prostate MP-MRI data of 20 patients, and the results of RD-CRF framework demonstrated a clear improvement with respect to the state-of-the-art in quantitative radiomics for automatic voxel-resolution prostate cancer detection.
关键词： multi-parametric magnetic resonance imaging (MP-MRI),Automatic prostate cancer detection,radiomics,feature model,conditional random fields (CRF)
更新于2025-09-23 15:21:01
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Plasmonic nano-dumbbells for enhanced photothermal and photodynamic synergistic damage of cancer cells
摘要： The longitudinal surface plasmon resonance of light-irradiated gold nanorods (Au NRs) is generated to enhance the local electric ?elds of Au NR-based nano-dumbbells (NDs), tailored speci?cally by coating mesoporous silica at two poles of Au NRs and embedding photosensitizer indocyanine green (ICG) into the mesopores. The assembled NDs possess a superior uniformity and water dispersity with a strong plasmonic absorption around 800 nm. Time-domain ?nite-difference calculations indicate that the enhanced local electric ?eld of NDs is predominantly distributed in the dumbbells at two poles of Au NRs, which improves the photonic performance of ICG signi?cantly. Illuminated by an 800 nm laser, the fabricated NDs demonstrate an enhanced combination of photothermal and photodynamic effects in comparison to either Au NRs or ICG alone. Synergistic damaging of photothermal and photodynamic combination to nasopharyngeal carcinoma cells has been corroborated experimentally, thus causing substantial cell death under a lower incident near-infrared laser power. This study concludes that the plasmonic NDs combined synergistically with ef?cient photothermal and photodynamic effects are highly promising in cancer therapy.
关键词： photodynamic therapy,photothermal therapy,cancer cells,gold nanorods,indocyanine green,Plasmonic nano-dumbbells
更新于2025-09-23 15:21:01
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Introducing New Conjugated Quantum Dots for Photothermal Therapy in Biological Applications
摘要： It is well-known that near-infrared (NIR) light sources are appropriate to ablate benign tumor irreversibly using heat treatment even in deep tissues. The laser light penetration into the skin in these wavelengths is deep (3–5 mm). Applying new stable materials for emitting NIR wavelengths in tumor positions can help cancer treatment. In this paper, synthesis of the conjugated core-multishell Ag/SiO2/Ag and Au/SiO2/Au quantum dots (QDs) with indocyanine green (ICG) is done and their theoretical and experimental absorptions and emissions in the NIR region are investigated. Thus, heat generation (high-resolution medical imaging capabilities) and emission enhancement are explained and described based on the FRET model for the proposed core-multishell QDs and it is shown that Ag/SiO2/Ag with ICG presents 4 times higher emission rate versus ICG alone in NIR region. Also, because of the plasmon hybridization and also resonance light penetration enhancement, the temperature in tissues increases that is useful for photothermal therapy and NIR high-resolution medical imaging for deep tissues. As an alternative application, these nanoparticles with amazing features are used as a heat source in cancer treatment for shallow and deep tissues. Finally, it is shown that Ag/SiO2/Ag QDs are the best solution for this purpose.
关键词： Cancer detection,FRET,Imaging,Quantum dots,Photothermal therapy
更新于2025-09-23 15:21:01
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Multifunctional Nanoparticle Loaded Injectable Thermoresponsive Hydrogel as NIR Controlled Release Platform for Local Photothermal Immunotherapy to Prevent Breast Cancer Postoperative Recurrence and Metastases
摘要： For breast cancer patients who have undergone breast-conserving surgery, effective treatments to prevent local recurrences and metastases is very essential. Here, a local injectable therapeutic platform based on a thermo-sensitive PLEL hydrogel with near-infrared (NIR)-stimulated drug release is developed to achieve synergistic photothermal immunotherapy for prevention of breast cancer postoperative relapse. Self-assembled multifunctional nanoparticles (RIC NPs) are composed of three therapeutic components including indocyanine green, a photothermal agent; resiquimod (R848), a TLR-7/8 agonist; and CPG ODNs, a TLR-9 agonist. RIC NPs are physically incorporated into the thermosensitive PLEL hydrogel. The RIC NPs encapsulated PLEL hydrogel (RIC NPs@PLEL) is then locally injected into the tumor resection cavity for local photothermal therapy to ablate residue tumor tissues and produce tumor-associated antigens. At the same time, NIR also triggers the release of immune components CPG ODNs and R848 from thermoresponsive hydrogels PLEL. The released immune components, together with tumor-associated antigens, work as an in situ cancer vaccine for postsurgical immunotherapy by inducing effective and sustained antitumor immune effect. Overall, this work suggests that photothermal immunotherapy based on local hydrogel delivery system has great potential as a promising tool for the postsurgical management of breast cancer to prevent recurrences and metastases.
关键词： cancer recurrence,thermal-responsive hydrogels,immunotherapy,controlled drug release,photothermal
更新于2025-09-23 15:21:01
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Near-infrared fluorescence-guided surgery using indocyanine green facilitates secure infrapyloric lymph node dissection during laparoscopic distal gastrectomy
摘要： Purpose We aimed to investigate the usefulness of near-infrared indocyanine green (ICG)-enhanced fluorescence guidance for infrapyloric LN dissection in laparoscopic distal gastrectomy. Methods This study enrolled patients with early gastric cancer scheduled for laparoscopic distal gastrectomy. After intra-operative submucosal injection of ICG (0.1 mg/mL), LN dissection was conducted under near-infrared ICG fluorescence guidance. The operation time, bleeding events during infrapyloric LN dissection were analyzed. Cases were retrospectively 1:3 propensity-score matched to patients who underwent laparoscopic distal gastrectomy without ICG injection. Results The mean time from midline omentectomy to exposure of the right gastroepiploic vein was significantly shorter in the ICG group (n = 20) than in the non-ICG group (n = 60) (13.05 ± 5.77 vs 18.68 ± 7.92 min; p = 0.001), and the incidence of bleeding during infrapyloric LN dissection was lower in the ICG group (20% vs 68.3%, p < 0.001). The two groups did not differ significantly regarding the number of LNs retrieved from the infrapyloric area (p = 0.434). Conclusions Near-infrared ICG fluorescence guidance facilitates safe and fast infrapyloric LN dissection in laparoscopic distal gastrectomy.
关键词： Indocyanine green,Laparoscopic surgery,Lymph node dissection,Near-infrared imaging,Gastric cancer
更新于2025-09-23 15:21:01