- 标题
- 摘要
- 关键词
- 实验方案
- 产品
过滤筛选
- 2018
- electromagnetic pulse
- cell proliferation
- cell membrane permeability
- cell response to electromagnetic stress
- apoptosis
- cancer therapy
- necrosis
- Intelligent Medical Engineering
- V.N. Karazin Kharkiv National University
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MICRO RAMAN SPECTROSCOPIC ANALYSIS ON BLOOD SERUM SAMPLES OF DUCTAL CARCINOMA PATIENTS
摘要： Objective: Identification of biochemical changes in ductal cancer patient’s serum samples using micro Raman spectroscopy. Methods: Micro Raman spectroscopy was used for the identification of Raman shift bands. Data analysis was done using K-means clustering. Results: Micro Raman spectroscopic analysis of human breast cancer patient’s serum samples was done. Biochemicals present in the samples were identified from the peak evaluations. K-means clustering analysis was used to differentiate the biochemicals present in the samples. Conclusion: From the study, we conclude that Raman spectroscopy has the potential to differentiate the biochemical changes occurring in the human body, and the differentiation can be done using K-means clustering.
关键词： Ductal cancer,Scattering,Spectroscopy,Blood serum
更新于2025-09-23 15:22:29
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Evaluating the Efficiency of Infrared Breast Thermography for Early Breast Cancer Risk Prediction in Asymptomatic Population
摘要： The high incidence and mortality rate of breast cancer in India and the limitations of gold standard method X-ray mammography to be used as a screening and diagnostic modality in young women tempted us to evaluate the efficiency of highly sensitive and non-radiating Infrared Breast Thermography (IBT) in early breast abnormality detection. This study investigates the efficiency of IBT by doing Temperature based analysis (TBA), Intensity based analysis (IBA), and Tumor Location Matching (TLM). In TBA and IBA, several temperature and intensity features were extracted from each thermogram to characterize healthy, benign and malignant breast thermograms. In TLM, the locations of suspicious regions in thermograms were matched with the tumor locations in mammograms / Fine Needle Aspiration Cytology images to prove the efficiency of IBT. Thirteen different sets of features have been created from the extracted temperature and intensity features and their classification performances have been evaluated by using Support Vector Machine with Radial basis function kernel. Among all feature sets, the feature set comprising the statistically significant (p<0.05) features provides the highest classification accuracy of 83.22% with sensitivity 85.56% and specificity 73.23%. Based on the results of this study, IBT is found to be potential enough to be used as a proactive technique for early breast abnormality detection in asymptomatic population and hence, capable of identifying the subjects that need urgent medical attention.
关键词： Routine check-up tool,Early breast abnormality prediction,Infrared breast thermography,Asymptomatic Patients,Breast cancer
更新于2025-09-23 15:22:29
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99mTc sestamibi SPECT: a possible tool for early detection of breast cancer lesions with high bone metastatic potential
摘要： The early identification of lesions with high metastatic potential by 99mTc sestamibi high-resolution SPECT analysis could be considered a new frontier for diagnosis and/or therapy of breast lesions.
关键词： SPECT,breast cancer,molecular imaging,breast osteoblast-like cells,99mTc sestamibi
更新于2025-09-23 15:22:29
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The capabilities and hope of the combination the new forms of personalized colon cancer treatment – immunotherapy and immune photodynamic therapy
摘要： Introduction: PDT can interfere with cytokine-mediated responses that play an important role in the processes of cancer progression, tumor angiogenesis and metastasis. Therefore, based on the identification of these cancer biomarkers, the therapy of combining various forms of treatment, including immunotherapy and PDT, may be a justified strategy for colorectal cancer treatment that focuses on individualized comprehensive therapy. Method: We reviewed the major approaches on the use of immunotherapy in colorectal cancer, with the special regard to photodynamic therapy, its immunological effect and new oncological treatment directions, connected with adjuvant immunotherapy including use of nanoparticles. Databases such as PubMed, ScienceDirect and Springer were utilized to search the literature for relevant articles. Purpose: To review studies of the immunotherapy in colon cancer and immune response to PDT. Conclusion: Based on the identification of immunological cancer biomarkers, the therapy of combining various forms of treatment, including immunotherapy and PDT, may be a justified strategy for colorectal cancer treatment that focuses on individualized comprehensive therapy.
关键词： cytokines,photodynamic therapy,colorectal cancer,immunotherapy
更新于2025-09-23 15:22:29
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Non-Invasive Fluorescent Monitoring of Ovarian Cancer in an Immunocompetent Mouse Model
摘要： Ovarian cancers (OCs) are the most lethal gynaecological malignancy, with high levels of relapse and acquired chemo-resistance. Whilst the tumour–immune nexus controls both cancer progression and regression, the lack of an appropriate system to accurately model tumour stage and immune status has hampered the validation of clinically relevant immunotherapies and therapeutic vaccines to date. To address this need, we stably integrated the near-infrared phytochrome iRFP720 at the ROSA26 genomic locus of ID8 mouse OC cells. Intrabursal ovarian implantation into C57BL/6 mice, followed by regular, non-invasive fluorescence imaging, permitted the direct visualization of tumour mass and distribution over the course of progression. Four distinct phases of tumour growth and dissemination were detectable over time that closely mimicked clinical OC progression. Progression-related changes in immune cells also paralleled typical immune profiles observed in human OCs. Specifically, we observed changes in both the CD8+ T cell effector (Teff):regulatory (Treg) ratio, as well as the dendritic cell (DC)-to-myeloid derived suppressor cell (MDSC) ratio over time across multiple immune cell compartments and in peritoneal ascites. Importantly, iRFP720 expression had no detectible influence over immune profiles. This new model permits non-invasive, longitudinal tumour monitoring whilst preserving host–tumour immune interactions, and allows for the pre-clinical assessment of immune profiles throughout disease progression as well as the direct visualization of therapeutic responses. This simple fluorescence-based approach provides a useful new tool for the validation of novel immuno-therapeutics against OC.
关键词： iRFP720,syngeneic,ovarian cancer,iRFP,tumour,T cell,immune,ID8
更新于2025-09-23 15:22:29
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Enhancement of cytotoxic effect on human head and neck cancer cells by?combination of photodynamic therapy and sulforaphan
摘要： Photodynamic therapy (PDT) is a method to treat cancers using photosensitizer and light. PDT has been tried for several tumors. However, the clinical applications are limited by the toxicity of photosensitizer and narrow effect. Sulforaphane (SFN) is a material of isothiocyanate group and known to have anticancer effect. We evaluated the cytotoxic effect of PDT combined with SFN on human head and neck cancer cells. We measured the cell viability, extent of apoptosis and necrosis, reactive oxygen species (ROS) generation and caspase activation. Cell viability was decreased significantly by combination treatment. Cellular apoptosis and necrosis were increased in combination treatment compared to SFN or PDT. ROS generation was also higher in combination treatment than single treatment. In combination treatment group, apoptosis and necrosis were decreased by administration of sodium azide (SA) which is scavenger of ROS. Increased caspase activation in combination treatment was also inhibited by SA. Combination of PDT and SFN led to enhanced cytotoxic effect on head and neck cancer cells. Combination treatment promoted the ROS generation, which induced cell death through activation of caspase pathway.
关键词： Sulforaphane,Reactive oxygen species,Head and neck cancer,Photodynamic therapy
更新于2025-09-23 15:22:29
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Dual-Modality ImmunoPET/Fluorescence Imaging of Prostate Cancer with an Anti-PSCA Cys-Minibody
摘要： Inadequate diagnostic methods for prostate cancer lead to over- and undertreatment, and the inability to intraoperatively visualize positive margins may limit the success of surgical resection. Prostate cancer visualization could be improved by combining the complementary modalities of immuno-positron emission tomography (immunoPET) for preoperative disease detection, and fluorescence imaging-guided surgery (FIGS) for real-time intraoperative tumor margin identification. Here, we report on the evaluation of dual-labeled humanized anti-prostate stem cell antigen (PSCA) cys-minibody (A11 cMb) for immunoPET/fluorescence imaging in subcutaneous and orthotopic prostate cancer models. Methods: A11 cMb was site-specifically conjugated with the near-infrared fluorophore Cy5.5 and radiolabeled with 124I or 89Zr. 124I-A11 cMb-Cy5.5 was used for successive immunoPET/fluorescence imaging of prostate cancer xenografts expressing high or moderate levels of PSCA (22Rv1-PSCA and PC3-PSCA). 89Zr-A11 cMb-Cy5.5 dual-modality imaging was evaluated in an orthotopic model. Ex vivo biodistribution at 24 h was used to confirm the uptake values, and tumors were visualized by post-mortem fluorescence imaging. Results: A11 cMb-Cy5.5 retained low nanomolar affinity for PSCA-positive cells. Conjugation conditions were established (dye-to-protein ratio of 0.7:1) that did not affect the biodistribution, pharmacokinetics, or clearance of A11 cMb. ImmunoPET using dual-labeled 124I-A11 cMb-Cy5.5 showed specific targeting to both 22Rv1-PSCA and PC3-PSCA s.c. xenografts in nude mice. Ex vivo biodistribution confirmed specific uptake to PSCA-expressing tumors with 22Rv1-PSCA:22Rv1 and PC3-PSCA:PC3 ratios of 13:1 and 5.6:1, respectively. Consistent with the immunoPET, fluorescence imaging showed a strong signal from both 22Rv1-PSCA and PC3-PSCA tumors compared with non-PSCA expressing tumors. In an orthotopic model, 89Zr-A11 cMb-Cy5.5 immunoPET was able to detect intraprostatically implanted 22Rv1-PSCA cells. Importantly, fluorescence imaging clearly distinguished the prostate tumor from surrounding seminal vesicles. Conclusion: Dual-labeled A11 cMb specifically visualized PSCA-positive tumor by successive immunoPET/fluorescence, which can potentially be translated for preoperative whole-body prostate cancer detection and intraoperative surgical guidance in patients.
关键词： molecular imaging,immunoPET,prostate cancer,antibody fragment,fluorescence
更新于2025-09-23 15:22:29
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Smart Supramolecular “Trojan Horse”-Inspired Nanogels for Realizing Light-Triggered Nuclear Drug Influx in Drug-Resistant Cancer Cells
摘要： Efficient nuclear delivery of anticancer drugs evading drug efflux transporters (DETs) on the plasma and nuclear membranes of multidrug-resistant cancer cells is highly challenging. Here, smart nanogels are designed via a one-step self-assembly of three functional components including a biocompatible copolymer, a fluorescent organosilica nanodot, and a photodegradable near-infrared (NIR) dye indocyanine green (ICG). The rationally designed nanogels have high drug encapsulation efficiency (≈99%) for anticancer drug doxorubicin (Dox), self-traceability for bioimaging, proper size for passive tumor targeting, prolonged blood circulation time for enhanced drug accumulation in tumor, and photocontrolled disassemblability. Moreover, the Dox-loaded nanogels can effectively kill multidrug-resistant cells via two steps: 1) They behave like a “Trojan horse” to escape from the DETs on the plasma membrane for efficiently transporting the anticancer “soldier” (Dox) into the cytoplasm and preventing the drugs from being excreted from the cells; 2) Upon NIR light irradiation, the photodegradation of ICG leads to the disassembly of the nanogels to release massive Dox molecules, which can evade the DETs on the nuclear membrane to exert their intranuclear efficacy in multidrug-resistant cells. Combined with their excellent biocompatibility, the nanogels may provide an alternative solution for overcoming cancer multidrug resistance.
关键词： nuclear delivery,photocontrollable drug release,cancer theranostics,silicon-based nanomaterials,supramolecular assembly
更新于2025-09-23 15:22:29
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Interventional Photothermal Therapy Enhanced Brachytherapy: A New Strategy to Fight Deep Pancreatic Cancer
摘要： Photothermal–radiotherapy (PT–RT) is an effective strategy for relieving hypoxia-related radiotherapy resistance and inducing tumor-specific cell apoptosis/necrosis. Nevertheless, limited tissue penetration of near-infrared (NIR) laser and the serious side effects of high-dose radiation severely hinder its applications for deep tumors. An interventional photothermal–brachytherapy (IPT–BT) technology is proposed here for the internal site-specific treatment of deep tumors. This technology utilizes a kind of biodegradable honeycomb-like gold nanoparticles (HGNs) acting as both internal photothermal agents and radiosensitizers. A high tumor inhibition rate of 96.6% is achieved in SW1990 orthotopic pancreatic tumor-bearing mice by HGNs-mediated IPT–BT synergistic therapy. Interestingly, this approach effectively causes double-stranded DNA damage and improves the oxygen supply and the penetration of nanoparticles inside the tumor. Therefore, it is believed that this strategy may open up a new avenue for PT–RT synergistic therapy of deep malignant tumors and has a significant impact on the future clinical translation.
关键词： photothermal therapy,pancreatic cancer,biodegradable,interventional,brachytherapy,honeycomb-like gold
更新于2025-09-23 15:22:29
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Fulguración ambulatoria con láser Holmium: Un procedimiento seguro para el tratamiento de la recidiva del carcinoma vesical no músculo infiltrante
摘要： Background and Objective: Nonmuscle invasive bladder cancer has a high recurrence rate and a low progression rate. The aim of this study was to assess the effectiveness, safety and feasibility of Holmium laser fulguration in an outpatient regimen for selected tumours. Material and Method: A prospective, longitudinal cohort study was conducted between January 2009 and December 2016. Seventy-nine Holmium laser fulguration procedures with subsequent instillation of mitomycin C were performed in an outpatient regimen on 59 patients with a history of low-risk bladder cancer and recurrence of small papillary tumours. We performed a descriptive data analysis and analysed the relapse-free time using Kaplan-Meier curves. Results: All procedures were completed in one day, and only one patient required subsequent hospitalisation due to haematuria. Some 87.2% of the patients presented pain with a visual analogue score ≤3. Recurrence occurred after 49.4% of the procedures (27.3% at 12 months). The median follow-up time was 17 months (range, 2-65). The onset of recurrence was significantly earlier after the second fulguration than after the first (median, 10 months vs. 56 months). Conclusions: Holmium laser fulguration and subsequent mitomycin C instillation in an outpatient regimen is a safe and feasible alternative to transurethral resection of bladder tumours in selected patients. Transurethral resection of the bladder tumour is recommended for patients with recurrence after fulguration, given the possibly higher risk of progression in these patients.
关键词： Nonmuscle invasive bladder cancer,Outpatient fulguration,Holmium laser
更新于2025-09-23 15:22:29