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- 摘要
- 关键词
- 实验方案
- 产品
过滤筛选
- 2018
- electromagnetic pulse
- cell proliferation
- cell membrane permeability
- cell response to electromagnetic stress
- apoptosis
- cancer therapy
- necrosis
- Intelligent Medical Engineering
- V.N. Karazin Kharkiv National University
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Synthesis and preclinical evaluation of 68Ga-PSMA-BCH for prostate cancer imaging
摘要： Prostate specific membrane antigen (PSMA) is a promising target for the diagnosis and therapy of prostate cancer. In this report, a NOTA-conjugated precursor, NOTA-PSMA (also named PSMA-BCH), was synthesized by peptide synthesizer with the chemical purity over 95%. 68Ga-PSMA-BCH was obtained by radiolabeling NOTA-PSMA with 68GaCl3 with >99% radiochemical purity and 59-74 GBq/μmol specific activity. In vitro and in vivo study of 68Ga-PSMA-BCH showed high stability, high uptake in PSMA-expressing cells and tumor, fast clearance and low non-target uptake. 22Rv1 tumors were clearly observed in micro-PET images of and showed good retention. Compared with 68Ga-PSMA-617, 68Ga-PSMA-BCH showed comparable tumor uptake and tumor-background ratios. Indicating 68Ga-PSMA-BCH is a promising candidate for prostate cancer imaging and worthy of further clinical investigations.
关键词： 68Ga-PSMA-BCH,PSMA,PET imaging,Prostate cancer
更新于2025-09-23 15:22:29
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Tumor recognition of peanut agglutinin-immobilized fluorescent nanospheres in biopsied human tissues
摘要： We are investigating an imaging agent for early detection of colorectal cancer. The agent, named the nanobeacon, is coumarin 6-encapsulated polystyrene nanospheres whose surfaces are covered with poly(N-vinylacetamide) and peanut agglutinin that reduces non-specific interactions with the normal mucosa and exhibits high affinity for terminal sugars of the Thomsen-Friedenreich antigen, which is expressed cancer-specifically on the mucosa, respectively. We expect that cancer can be diagnosed by detecting illumination of intracolonically administered nanobeacon on the mucosal surface. In the present study, biopsied human tissues were used to evaluate the potential use of the nanobeacon in the clinic. Prior to the clinical study, diagnostic capabilities of the nanobeacon for detection of colorectal cancer were validated using 20 production batches whose characteristics were fine-tuned chemically for the purpose. Ex vivo imaging studies on 66 normal and 69 cancer tissues removed from the colons of normal and orthotopic mouse models of human colorectal cancer, respectively, demonstrated that the nanobeacon detected colorectal cancer with excellent capabilities whose rates of true and false positives were 91% and 5%, respectively. In the clinical study, normal and tumor tissues on the large intestinal mucosa were biopsied endoscopically from 11 patients with colorectal tumors. Histological evaluation revealed that 9 patients suffered from cancer and the rest had adenoma. Mean fluorescence intensities of tumor tissues treated with the nanobeacon were significantly higher than those of the corresponding normal tissues. Correlation of magnitude relation of the intensity in individuals was observed in cancer patients with a high probability (89%); however, the probability reduced to 50% in adenoma patients. There was a reasonable likelihood for diagnosis of colorectal cancer by the nanobeacon applied to the mucosa of the large intestine.
关键词： Colorectal cancer,Diagnostic agent,Optical imaging,Peanut agglutinin,Biomarker imaging,Nanosphere,Thomsen-Friedenreich antigen
更新于2025-09-23 15:22:29
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Functionalized fluorescent carbon nanoparticles for sensitively targeted of folate-receptor-positive cancer cells
摘要： The folic acid-functionalized fluorescent carbon dots (FA-CDs) was synthesized via the assembly of FA to the surface of CDs. A facile hydrothermal method with proline and ethylenediamine as precursors was used to fabricate CDs. The as-prepared CDs possessed active amino groups where the CDs could be further engineered for the conjugation with FA. The uptake of the as-synthesized FA-CDs by FR positive MCF-7 cells (FR++) and HepG-2 cells (FR+) via receptor-mediated endocytosis was demonstrated by confocal laser scanning microscopy, which is further verified by a comparative study with FR-negative PC-12 cells (FR-). The bright fluorescence can be observed in FR positive MCF-7 cells while negligible fluorescence was observed in PC-12 cells with low-expressed FR, demonstrating that FA-CDs could accurately identify FR-positive cancer cells from normal cells. The FA-CDs shared favorable biocompatibility, excellent optical properties and ultra-low toxicity etc. Holding these superior properties, the FA-CDs was implemented as a highly effective platform for biological labeling and imaging, which may provide a innovative vision for cancer diagnosis and succeeding personalized therapy.
关键词： Folic acid-functionalized,Targeted bioimaging,Cancer cells,Carbon dots
更新于2025-09-23 15:22:29
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Tuning of carbon dots emission color for sensing of Fe3+ ion and bioimaging applications
摘要： Herein, we report a facile one-step synthetic strategy for fabrication of three (blue, green and yellow) fluorescent color carbon dots (CDs) from tomato (Solanum lycopersicum). The as-synthesized CDs showed emission peaks at 450, 520 and 560 nm for blue, green and yellow color CDs when excited at 370, 420 and 460 nm, respectively. Using tomato as a carbon source, the fabricated three fluorescent color CDs showed good water dispersity and high quantum yield. The analytical performances of three fluorescent color CDs are evaluated by detecting Fe3+ ion in biofluids and iron tablets. Upon the addition of Fe3+ ion under optimal conditions, the fluorescence intensity of three fluorescent color CDs was quenched linearly over the range of 0.1 to 2.0 μM. This method opens a new analytical strategy to quantify Fe3+ ion in iron tablets and biofluids with high sensitivity. Further, the uptake of three fluorescent color CDs into HeLa cells was confirmed by confocal laser scanning microscopy. Intracellular experiments demonstrated that the three fluorescent color CDs were effectively internalized the cells and show excellent biocompatibility and low toxicity, suggesting that the CDs can be used as good candidates for biomedical applications.
关键词： Fluorescent carbon dots,Fe3+ ion,Cancer cells,Fluorescence spectroscopy and microscopy,Tomato
更新于2025-09-23 15:22:29
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Temperature induced two new trinuclear Co(II) cluster-based compounds: Luminescent, photocatalytic and anti-breast cancer properties
摘要： Two new trinuclear Co(II) cluster-based compounds, namely [Co3(L)2(H2O)4]n (1) and [Co1.5(L)(H2O)2]n (2) (H3L=biphenyl-3,4',5-tricarboxylic acid), have been synthesized under hydrothermal conditions via tuning reaction temperature. Single crystal X-ray diffraction analyses revealed that both compound 1 and 2 contain trinuclear Co(II) cluster subunits: trinuclear [Co3(COO)4(H2O)2] cluster for 1 and trinuclear [Co3(COO)6] cluster for 2. Due to the different coordination modes of L3- ligand, compound 1 features a (3,6)-connected 3D framework with a rtl-type topology, and compound 2 features a (3,6)-connected 3D framework with a new topology which has not been reported in the TOPOS and RCSR database. The thermal stabilities and luminescent properties for the two compounds have been investigated. The photophysical properties studies showed that compounds 1 and 2 are excellent candidates as potential semiconductive materials. Moreover, compounds 1 and 2 exhibit high photocatalytic efficiency for the degradation of methylene blue (MB) under UV light irradiation. In addition, the cytotoxicity of complexes 1 and 2 has been evaluated against three human breast cancer cells (MCF-7, MDA-MB-231 and BT-549) via the MTT assay.
关键词： Breast cancer,Photocatalysis,Co(II) compound,Luminescence
更新于2025-09-23 15:22:29
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18F-FDG PET/TC para la estadificación y la delineación del volumen de radioterapia en el cáncer de cabeza y cuello
摘要： Purpose: The aim is to investigate the use of 18F-FDG (fluorine-18 fluorodeoxyglucose) PET/CT in head and neck cancer (HNC) staging and its effect on the therapeutic strategy and radiotherapy (RT) planning. Methods and materials: One hundred patients with HNC were included. Primary tumor sites: 18% oral cavity, 20% oropharynx, 12% hypopharynx, 11% nasopharynx, 37% larynx, 2% paranasal sinuses. Patients were staged according to the American Joint Committee of Cancer 7th edition. Stage: 5% stage I, 7% stage II, 14% stage III, 61% stage IVA, 7% stage IVB and 6% stage IVC. A contrast-enhanced CT and a 18F-FDG PET/CT acquired under RT position were performed. Both exams were compared to analyze patients' staging reclassification. Changes in therapeutic strategy were analyzed. Results: 18F-FDG PET/CT detected 6 distant metastases and treatment intention changed to palliative. Eight synchronous tumors were detected; one received palliative treatment. 18F-FDG PET/CT reclassified cTNM staging in 27 patients. Tumor extension changed in 28 (14% up-staged; 14% down-staged), implying a change in GTV (Gross Tumor Volume) delineation. Nodal detection was reclassified in 47 patients: 8 patients down-staged (N2C to N2A/N2B/N1) and 2 were false positive. Nineteen patients were false negatives and 5 staged as N+ (N1/N2A/N2B) turned out into N2C. These staging modifications imply adapting the nodal volume to be irradiated. Conclusions: 18F-FDG PET/CT reclassification was higher than 10% in almost all categories studied (cTNM, tumor extension and nodal disease) and detects more metastases and synchronous tumors than conventional studies, which has an impact on the therapeutic patient management and RT planning.
关键词： Head and neck cancer,18F-FDG PET/CT,Radiotherapy planning,Staging
更新于2025-09-23 15:22:29
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Expression of peptide transporter 1?has a positive correlation in protoporphyrin IX accumulation induced by 5-aminolevulinic acid with photodynamic detection of non-small cell lung cancer and metastatic brain tumor specimens originating from non-small cell lung cancer
摘要： BACKGROUND: Recently, 5-aminolevulinic acid (5-ALA)-induced protoporphyrin IX fluorescence was reported to be an useful tool during total surgical resection of high-grade gliomas. However, the labeling efficacy of protoporphyrin IX fluorescence is lower in metastatic brain tumors compared to that in high-grade gliomas, and the mechanism underlying protoporphyrin IX fluorescence in metastatic brain tumors remains unclear. Lung cancer, particularly non-small cell lung cancer (NSCLC), is the most common origin for metastatic brain tumor. Therefore, we investigated the mechanism of protoporphyrin IX fluorescence in NSCLC and associated metastatic brain tumors. METHODS: Western blotting and quantitative real-time polymerase chain reaction (qRT-PCR) was employed to evaluate the protein and mRNA levels of five transporters and enzymes involved in the porphyrin biosynthesis pathway: peptide transporter 1 (PEPT1), hydroxymethylbilane synthase (HMBS), ferrochelatase (FECH), ATP-binding cassette 2 (ABCG2), and heme oxygenase 1 (HO-1). The correlation between protein, mRNA, and protoporphyrin IX levels in NSCLC cells were evaluated in vitro. Immunohistochemistry was used to determine proteins that played a key role in intraoperative protoporphyrin IX fluorescence in clinical samples from patients with NSCLC and pathologically confirmed metastatic brain tumors. RESULTS: A significant correlation between PEPT1 expression and protoporphyrin IX accumulation in vitro was identified by western blotting (P = 0.003) and qRT-PCR (P = 0.04). Immunohistochemistry results indicated that there was a significant difference in PEPT1 between the intraoperative protoporphyrin IX fluorescence-positive and protoporphyrin IX fluorescence-negative groups (P = 0.009). CONCLUSION: Expression of PEPT1 was found to be positively correlated with 5-ALA-induced protoporphyrin IX accumulation detected by photodynamic reaction in metastatic brain tumors originating from NSCLC.
关键词： protoporphyrin IX,brain metastasis,non-small lung cancer,peptide transporter 1
更新于2025-09-23 15:22:29
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Cancer Selective Turn-on Fluorescence Imaging Using a Biopolymeric Nanocarrier
摘要： Most nanoparticle-based bioresearch for clinical applications is unable to overcome the clinical barriers of efficacy (e.g., sensitivity and selectivity), safety for human use, and mass-production processes. Here, we proposed a promising concept of using a biocompatible nanocarrier that delivers natural fluorescent precursors into cancerous cells. The nanocarrier is a biopolymeric nanoparticle that can be easily loaded with fluorescent precursors to form a fluorescent moiety via a biosynthesis pathway. Once delivered into cancerous cells, the nanocarriers are selectively turned on and distinctively fluoresce upon excitation. We, therefore, demonstrated the efficacy of the selective turn-on fluorescence of the nanocarriers in in vitro co-culture models and in vivo tumor-bearing models.
关键词： Hyaluronic Acid,Cancer Diagnosis,Biocompatible Nanocarrier,Turn-on Fluorescence,5-Aminolevulinic Acid
更新于2025-09-23 15:22:29
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Theranostic Carbon Dots with Innovative NIR-II Emission for in Vivo Renal Excreted Optical Imaging and Photothermal Therapy
摘要： Carbon dots (CDs) with low biotoxicity, high photostability, and well-controlled small size are highly desirable imaging agents for optical bioimaging. However, most of the CDs triggered by ultraviolet (UV)/blue light present visible/first near infrared (NIR-I) emissions shorter than 820 nm, impairing their imaging applications in vivo by low penetration depth. Hence, developing novel CDs based materials with second near infrared (NIR-II) emission located in 1000-1700 nm region is an urgent task. Here, a novel NIR-II emitting CDs-based nanoprobe triggered by 808 nm laser is developed. The designed CDs with 900-1200 nm luminescence possess high quantum yield (QY-0.4%), and high biocompatibility, which have proven to be effective probes for in vivo NIR-II bioimaging. Notably, nearly 65% CDs are excreted from mouse urine within 6 h, further demonstrating the rapid renal clearance of CDs. Furthermore, the designed CDs also exhibit high photothermal efficiency (30.6%), making them ideal materials for thermal ablation of cancer. Our findings pave the way of designing multifunctional CDs-based theranostic platform for simultaneously integrating the advanced NIR-II bioimaging and photothermal therapy (PTT) of cancer.
关键词： carbon dots,renal clearance,photothermal therapy of cancer,NIR-II bioimaging,NIR-triggered NIR-II emission
更新于2025-09-23 15:22:29
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Near-Infrared Light Triggered Sulfur Dioxide Gas Therapy of Cancer
摘要： The exploitation of gas therapy platforms holds great promise as a 'green' approach for selective cancer therapy, however, it is often associated with some challenges, such as uncontrolled or insufficient gas generation and unclear therapeutic mechanisms. In this work, a gas therapy approach based on near-infrared (NIR) light triggered sulfur dioxide (SO2) generation was developed, and the therapeutic mechanism as well as in vivo anti-tumor therapeutic efficacy was demonstrated. A SO2 prodrug-loaded rattle-structured upconversion@silica nanoparticles (RUCSNs) was constructed to enable high loading capacity without obvious leakage, and to convert NIR light into ultraviolet (UV) light so as to activate the prodrug for SO2 generation. In addition, SO2 prodrug-loaded RUCSNs showed high cell uptake, good biocompatibility, intracellular tracking ability, and high NIR light triggered cytotoxicity. Furthermore, the cytotoxic SO2 was found to induce cell apoptosis accompanied with the increase of intracellular reactive oxygen species (ROS) levels and the damage of nuclear DNA. Moreover, efficient inhibition of tumor growth was achieved, associated with significantly prolonged survival of mice. Such NIR light-triggered SO2 therapy may provide an effective strategy to stimulate further development of synergistic cancer therapy platforms.
关键词： upconversion nanoparticles (UCNPs),gas therapy,cancer therapy,near-infrared (NIR),sulfur dioxide (SO2)
更新于2025-09-23 15:22:29