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Stilbene derivative as a photosensitive compound to control the excitability of neonatal rat cardiomyocytes
摘要： Substances that can be used as photosensitizers for cardiac tissue are very helpful in modeling various excitation patterns in a cardiac tissue culture and may have prospective use in the temporary and permanent ablation of unwanted excitation sources in the heart. The aim of the present work is to study the effect of stilbene derivative c-TAB (2- {4- [(E) -2- (4-ethoxyphenyl) vinyl] phenoxy} ethyl) trimethylammonium bromide) on the cardiomyocyte layers and voltage-gated ion channels in cardiac cells. C-TAB is a structural analogue to AzoTAB, reported previously as a photoswitch for cardiac and neural cells, in which the azobenzene moiety is replaced by a stilbene grouping. Such a replacement makes c-TAB less toxic to living cells. c-TAB has been shown to successfully inhibit excitation in cardiac cells in both trans- and cis- forms. The excitation inhibition of cardiac cells under c-TAB is reversible and can be overturned easily by washing out the c-TAB; however, not by light illumination. The irradiation of cardiac cells with near-UV when the trans- form of c-TAB is applied changes reversible inhibition to a permanent one that cannot be overturned by a washout.
关键词： Excitation wave propagation,Stilbene derivative,Voltage-gated Ion channels,Neonatal rat cardiomyocyte
更新于2025-09-23 15:22:29
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Potassium channel-based optogenetic silencing
摘要： Optogenetics enables manipulation of biological processes with light at high spatio-temporal resolution to control the behavior of cells, networks, or even whole animals. In contrast to the performance of excitatory rhodopsins, the effectiveness of inhibitory optogenetic tools is still insufficient. Here we report a two-component optical silencer system comprising photo-activated adenylyl cyclases (PACs) and the small cyclic nucleotide-gated potassium channel SthK. Activation of this ‘PAC-K’ silencer by brief pulses of low-intensity blue light causes robust and reversible silencing of cardiomyocyte excitation and neuronal firing. In vivo expression of PAC-K in mouse and zebrafish neurons is well tolerated, where blue light inhibits neuronal activity and blocks motor responses. In combination with red-light absorbing channelrhodopsins, the distinct action spectra of PACs allow independent bimodal control of neuronal activity. PAC-K represents a reliable optogenetic silencer with intrinsic amplification for sustained potassium-mediated hyperpolarization, conferring high operational light sensitivity to the cells of interest.
关键词： Potassium channel,Neuronal silencing,Photo-activated adenylyl cyclases,Optogenetics,Cardiomyocyte excitation
更新于2025-09-23 15:21:21
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Non-Invasive Plasmonic Based Real Time Characterization of Cardiac Drugs on Cardiomyocytes Functional Behavior
摘要： In fabrication of cardiac tissue, an important factor is continuous measurement of its contraction features. A module that allows for a dynamic system capable of non-invasive and label-free monitoring of contraction profile under administering chemicals and drugs, is highly valuable for understanding accurate tissue mechanobiology. In this research, we have successfully demonstrated the use of Surface Plasmon Resonance (SPR) technology for the first time to characterize the contractility of cardiac cells in response to Blebbistatin and ATP as drug exposure in real-time. An optimal flow rate of 10 μlit/min was selected for continuous flow of warm media and 10 μM drug administration effect was detected with high spatiotemporal sensitivity on contracting cardiomyocytes. Our drug screening has identified the source of SPR periodic signal to be directly cell contraction rather than action potentials or calcium signaling. Per our results, SPR has high potential in applications in least interference real-time and label-free tissue characterizations and cellular properties analysis from a functional and structural point of view.
关键词： drug development,cardiomyocyte contraction,surface plasmon resonance,in-vitro,label-free,biosensor,imaging
更新于2025-09-12 10:27:22
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Cardiomyocyte-Driven Structural Color Actuation in Anisotropic Inverse Opals
摘要： Biohybrid actuators composed of living tissues and artificial materials have attracted increasing interest in recent years because of their extraordinary function of dynamically sensing and interacting with complex bioelectrical signals. Here, a compound biohybrid actuator with self-driven actuation and self-reported feedback is designed based on an anisotropic inverse opal substrate with periodical elliptical macropores and a hydrogel filling. The benefit of the anisotropic surface topography and high biocompatibility of the hydrogel is that the planted cardiomyocytes could be induced into a highly ordered alignment with recovering autonomic beating ability on the elastic substrate. Because of the cell elongation and contraction during cardiomyocyte beating, the anisotropic inverse opal substrates undergo a synchronous cycle of deformation actuations, which can be reported as corresponding shifts of their photonic band gaps and structural colors. These self-driven biohybrid actuators could be used as elements for the construction of soft-bodied structural color robot, such as a biomimetic guppy with a swinging tail. Besides, with the integration of a self-driven biohybrid actuator and microfluidics, the advanced heart-on-a-chip system with the feature of microphysiological visuality has been developed for integrated cell monitoring and drug testing. This anisotropic inverse opal-derived biohybrid actuator could be widely applied in biomedical engineering.
关键词： cardiomyocyte,microfluidics,inverse opal,heart on a chip,structural color,actuator
更新于2025-09-04 15:30:14